Name: iFluor® IRD 800 CW Maleimide
Brand: AAT Bioquest
SKU: 71612
Availability: InStock

iFluor® IRD 800 CW Maleimide

iFluor® IRD 800 CW maleimide is a near-infrared thiol-reactive dye chemically identical to IRDye® 800CW (LI-COR) that enables site-specific protein labeling for in vivo imaging with minimal background fluorescence.

Thiol-Reactive NIR Probe: Maleimide group forms stable thioether bonds with cysteines
Deep Tissue Imaging: Ex/Em = 774/789 nm penetrates >1 cm tissue with minimal scatter
pH-Stable Conjugation: Reactive at pH 6.5–7.5 for site-specific protein labeling

iFluor® IRD 800 CW Maleimide has the same chemical structure as the IRDye® 800 CW Maleimide (IRDye® is the trademark of LI-COR).

Protocol

Catalog	Size	Price	Quantity
71612	1 mg	Price
71613	5 mg	Price

Overview

iFluor® IRD 800 CW Maleimide has the same chemical structure as the IRDye® 800 CW Maleimide (IRDye® is the trademark of LI-COR). IRD 800CW Maleimide is a thiol-reactive near-infrared (NIR) fluorescent dye with good water solubility. It can be used to label peptides, antibodies, and other thiol-containing biomolecules such as thiol-modified oligos. The IRD 800CW conjugates can be used as a probe for Western blot, flow cytometry, immunocytochemical assays, including In-Cell Western assays and On-Cell Western cell-based assays, protein arrays, microscopy, tissue section imaging, and in vivo imaging. IRD 800CW dye-conjugated probes are also used for fluorescence imaging-based cancer diagnosis.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® IRD 800 CW Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	80.689 µL	403.444 µL	806.888 µL	4.034 mL	8.069 mL
5 mM	16.138 µL	80.689 µL	161.378 µL	806.888 µL	1.614 mL
10 mM	8.069 µL	40.344 µL	80.689 µL	403.444 µL	806.888 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1239.33 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Bionanocomposite MIL-100(Fe)/Cellulose as a high-performance adsorbent for the adsorption of methylene blue.

Authors:

Abbasi, Shahla and Nezafat, Zahra and Javanshir, Shahrzad and Aghabarari, Behzad

Journal:

Scientific reports (2024): 14497

Experimental upper bounds for resonance-enhanced entangled two-photon absorption cross section of indocyanine green.

Authors:

He, Manni and Hickam, Bryce P and Harper, Nathan and Cushing, Scott K

Journal:

The Journal of chemical physics (2024)

Eco-friendly biosynthesis of TiO2 nanoparticles using Desmostachya bipinnata extract: Larvicidal and pupicidal potential against Aedes aegypti and Spodoptera litura and acute toxicity in non-target organisms.

Authors:

Shyam-Sundar, Narayanan and Karthi, Sengodan and Senthil-Nathan, Sengottayan and Narayanan, Kilapavoor Raman and Santoshkumar, Balasubramanian and Sivanesh, Haridoss and Chanthini, Kanagaraj Muthu-Pandian and Stanley-Raja, Vethamonickam and Ramasubramanian, Ramakrishnan and Abdel-Megeed, Ahmed and Malafaia, Guilherme

Journal:

The Science of the total environment (2023): 159512

Development of ratiometric fluorescent probes based on peptides for sensing Pb2+ in aquatic environments and human serum.

Authors:

Lee, Jae Yoon and Mehta, Pramod Kumar and Subedi, Sumita and Lee, Keun-Hyeung

Journal:

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (2023): 122502

Pyrolyzed magnetic NiO/carbon-derived nanocomposite from a hierarchical nickel-based metal-organic framework with ultrahigh adsorption capacity.

Authors:

Beigi, Negar and Shayesteh, Hadi and Javanshir, Shahrzad and Hosseinzadeh, Majid

Journal:

Environmental research (2023): 116146

Physical properties

Molecular weight	1239.33
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.3
Correction factor (280 nm)	0.3
Extinction coefficient (cm ^-1 M ^-1)	240000 ¹
Excitation (nm)	774
Emission (nm)	792
Quantum yield	0.09

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on August 29, 2026