iFluor® 860 succinimidyl ester

iFluor® 860 succinimidyl ester is an amine-reactive, near-infrared fluorescent dye designed for covalent labeling of proteins, particularly antibodies, for fluorescence imaging and detection applications.

Amine-Reactive Labeling: Covalently conjugates to primary amino groups of proteins.
Spectral Properties: Excitation/emission maxima of 853/878 nm.
Spectral Compatibility: Infrared emission is well separated from Cy5, Cy7, and APC fluorophores.
Applications: Suitable for small-animal in vivo imaging and multicolor fluorescence analysis.

<p>Fluorescent dye NHS esters (or succinimidyl esters) are the most popular tool for conjugating dyes to a peptide, protein, antibody, amino-modified oligonucleotide or nucleic acid. NHS esters react readily with the primary amines (R-NH<sub>2</sub>) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. The resulting dye conjugates (e.g., iFluor 860-antibodies) are quite stable.</p>

Protocol

Catalog	Size	Price
1409	1 mg	Price
71409	100 ug	Price
71529	5 mg	Price
71579	10 mg	Price

Overview

In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 860 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 860 have excitation and emission in the IR range. iFluor® 860 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detection. iFluor® 860 succinimidyl ester is amine-reactive and can be readily used to conjugate amine-containing biomolecules, particularly antibodies.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 860 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	63.617 µL	318.086 µL	636.173 µL	3.181 mL	6.362 mL
5 mM	12.723 µL	63.617 µL	127.235 µL	636.173 µL	1.272 mL
10 mM	6.362 µL	31.809 µL	63.617 µL	318.086 µL	636.173 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1571.9 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

Regulatory T cells in the primary tumor growth lesion in colorectal cancer

Authors:

Kryukova, VV and Tsepelev, VL and Tereshkov, PP

Journal:

Advances in Molecular Oncology (2025): 100--108

Identification of distinct functional thymic programming of fetal and pediatric human $\gamma$$\delta$ thymocytes via single-cell analysis

Authors:

Sanchez Sanchez, Guillem and Papadopoulou, Maria and Azouz, Abdulkader and Tafesse, Yohannes and Mishra, Archita and Chan, Jerry KY and Fan, Yiping and Verdebout, Isoline and Porco, Silvana and Libert, Fr{\'e}d{\'e}rick and others

Journal:

Nature communications (2022): 1--19

Gas plasma irradiation of breast cancers promotes immunogenicity, tumor reduction, and an abscopal effect in vivo

Authors:

Mahdikia, Hamed and Saadati, Fariba and Freund, Eric and Gaipl, Udo S and Majidzadeh-a, Keivan and Shokri, Babak and Bekeschus, Sander

Journal:

OncoImmunology (2021): 1859731

Nanovesicle delivery to the liver via retinol binding protein and platelet-derived growth factor receptors: how targeting ligands affect biodistribution

Authors:

Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You

Journal:

Nanomedicine (2017)

17-Marker, 18-Color Human Blood Phenotyping Made Easy with Flow Cytometry

Authors:

McCracken, James and Lawson, Jonel

References

View all 18 references: Citation Explorer

In Vivo Fluorescence Microscopic Imaging for Dynamic Quantitative Assessment of Intestinal Mucosa Permeability in Mice

Authors:

Szabo A, Vollmar B, Boros M, Menger MD.

Journal:

J Surg Res. (2007)

A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate

Authors:

Hama Y, Urano Y, Koyama Y, Kamiya M, Bernardo M, Paik RS, Shin IS, Paik CH, Choyke PL, Kobayashi H.

Journal:

Cancer Res (2007): 2791

Fluorescence imaging in vivo: recent advances

Authors:

Rao J, Dragulescu-Andrasi A, Yao H.

Journal:

Curr Opin Biotechnol (2007): 17

Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis

Authors:

Steenkeste K, Lecart S, Deniset A, Pernot P, Eschwege P, Ferlicot S, Leveque-Fort S, Bri and et R, Fontaine-Aupart MP.

Journal:

Photochem Photobiol (2007): 1157

In vivo monitoring the fate of Cy5.5-Tat labeled T lymphocytes by quantitative near-infrared fluorescence imaging during acute brain inflammation in a rat model of experimental autoimmune encephalomyelitis

Authors:

Berger C, Gremlich HU, Schmidt P, Cannet C, Kneuer R, Hiest and P, Rausch M, Rudin M.

Journal:

J Immunol Methods (2007): 65

Physical properties

Molecular weight	1571.90
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.1
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	250000 ¹
Excitation (nm)	853
Emission (nm)	878

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on November 9, 2026