iFluor® 488 azide
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 716.57 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
Molecular weight 716.57 | Correction Factor (260 nm) 0.21 | Correction Factor (280 nm) 0.11 | Extinction coefficient (cm -1 M -1) 750001 | Excitation (nm) 491 | Emission (nm) 516 | Quantum yield 0.91 |
Although FITC is still the most popular fluorescent labeling dye for preparing green fluorescent bioconjugates, there are certain limitations with FITC, such as severe photobleaching for microscope imaging and pH-sensitive fluorescence. Protein conjugates prepared with iFluor® 488 dyes are far superior to conjugates of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 azide dye is reasonably stable and shows good reactivity and selectivity with the alkyne group for click chemistry applications. The iFluor® 488 dyes are an excellent replacement to the Alexa Fluor dyes that have identical spectral properties ( Alexa Fluor® is the trademark of Invitrogen).
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 139.554 µL | 697.769 µL | 1.396 mL | 6.978 mL | 13.955 mL |
5 mM | 27.911 µL | 139.554 µL | 279.107 µL | 1.396 mL | 2.791 mL |
10 mM | 13.955 µL | 69.777 µL | 139.554 µL | 697.769 µL | 1.396 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.21 |
Correction Factor (280 nm) | 0.11 |
Extinction coefficient (cm -1 M -1) | 750001 |
Excitation (nm) | 491 |
Emission (nm) | 516 |
Quantum yield | 0.91 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 647 azide | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 488 tyramide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 azide | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 TCO | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 Tetrazine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor®488-dUTP *1 mM in Tris Buffer (pH 7.5)* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor™ 405 azide | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor™ 790 Azide | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
Images
Figure 1. Click chemistry is a method for attaching a probe or substrate of interest to a specific biomolecule, a process called bioconjugation. The possibility of attaching fluorophores and other reporter molecules has made click chemistry a very powerful tool for identifying, locating, and characterizing both old and new biomolecules. The classic click reaction is the copper-catalyzed reaction of an azide with an alkyne to form a 5-membered heteroatom ring, this reaction is commonly called Cu(I)-Catalyzed Azide-Alkyne Cycloaddition (CuAAC).
Citations
View all 28 citations: Citation Explorer
Intestinal inflammation marker calprotectin regulates epithelial intestinal zinc metabolism and proliferation in mouse jejunal organoids
Authors: Gonz{\'a}lez, R and Ceacero-Heras, D and Tena-Garitaonaindia, M and {\'A}lvarez-Mercado, A and G{\'a}mez-Belmonte, R and Chazin, WJ and de Medina, F S{\'a}nchez and Mart{\'\i}nez-Augustin, O
Journal: Biomedicine \& Pharmacotherapy (2024): 116555
Authors: Gonz{\'a}lez, R and Ceacero-Heras, D and Tena-Garitaonaindia, M and {\'A}lvarez-Mercado, A and G{\'a}mez-Belmonte, R and Chazin, WJ and de Medina, F S{\'a}nchez and Mart{\'\i}nez-Augustin, O
Journal: Biomedicine \& Pharmacotherapy (2024): 116555
A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms
Authors: Zhang, Liming and Liu, Jieyu and Huang, Shisi and Zeng, Wentao and Li, Li and Fan, Xihao and Lu, Zhuoxuan
Journal: Analytica Chimica Acta (2024): 342416
Authors: Zhang, Liming and Liu, Jieyu and Huang, Shisi and Zeng, Wentao and Li, Li and Fan, Xihao and Lu, Zhuoxuan
Journal: Analytica Chimica Acta (2024): 342416
Pharmacological inhibition of RAS overcomes FLT3 inhibitor resistance in FLT3-ITD+ AML through AP-1 and RUNX1
Authors: Coleman, Daniel JL and Keane, Peter and Chin, Paulynn S and Ames, Luke and Kellaway, Sophie and Blair, Helen and Khan, Naeem and Griffin, James and Holmes, Elizabeth and Maytum, Alexander and others,
Journal: iScience (2024)
Authors: Coleman, Daniel JL and Keane, Peter and Chin, Paulynn S and Ames, Luke and Kellaway, Sophie and Blair, Helen and Khan, Naeem and Griffin, James and Holmes, Elizabeth and Maytum, Alexander and others,
Journal: iScience (2024)
FAM81A is a postsynaptic protein that regulates the condensation of postsynaptic proteins via liquid--liquid phase separation
Authors: Kaizuka, Takeshi and Hirouchi, Taisei and Saneyoshi, Takeo and Shirafuji, Toshihiko and Collins, Mark O and Grant, Seth GN and Hayashi, Yasunori and Takumi, Toru
Journal: Plos Biology (2024): e3002006
Authors: Kaizuka, Takeshi and Hirouchi, Taisei and Saneyoshi, Takeo and Shirafuji, Toshihiko and Collins, Mark O and Grant, Seth GN and Hayashi, Yasunori and Takumi, Toru
Journal: Plos Biology (2024): e3002006
Phosphorylation-dependent membraneless organelle fusion and fission illustrated by postsynaptic density assemblies
Authors: Wu, Haowei and Chen, Xudong and Shen, Zeyu and Li, Hao and Liang, Shiqi and Lu, Youming and Zhang, Mingjie
Journal: Molecular Cell (2023)
Authors: Wu, Haowei and Chen, Xudong and Shen, Zeyu and Li, Hao and Liang, Shiqi and Lu, Youming and Zhang, Mingjie
Journal: Molecular Cell (2023)
m6A-modified circNFIX promotes ovarian cancer progression and immune escape via activating IL-6R/JAK1/STAT3 signaling by sponging miR-647
Authors: Wang, Ruiyu and Ye, Hui and Yang, Bowen and Ao, Mengyin and Yu, Xiuzhang and Wu, Yuke and Xi, Mingrong and Hou, Minmin
Journal: International Immunopharmacology (2023): 110879
Authors: Wang, Ruiyu and Ye, Hui and Yang, Bowen and Ao, Mengyin and Yu, Xiuzhang and Wu, Yuke and Xi, Mingrong and Hou, Minmin
Journal: International Immunopharmacology (2023): 110879
Brassica napus Roots Use Different Strategies to Respond to Warm Temperatures
Authors: Boter, Marta and Pozas, Jenifer and Jarillo, Jose A and Pi{\~n}eiro, Manuel and Pernas, M{\'o}nica
Journal: International Journal of Molecular Sciences (2023): 1143
Authors: Boter, Marta and Pozas, Jenifer and Jarillo, Jose A and Pi{\~n}eiro, Manuel and Pernas, M{\'o}nica
Journal: International Journal of Molecular Sciences (2023): 1143
Vpx requires active cellular dNTP biosynthesis to effectively counteract the anti-lentivirus activity of SAMHD1 in macrophages.
Authors: Bowen, Nicole E and Tao, Sijia and Cho, Young-Jae and Schinazi, Raymond F and Kim, Baek
Journal: Journal of Biological Chemistry (2023): 104984
Authors: Bowen, Nicole E and Tao, Sijia and Cho, Young-Jae and Schinazi, Raymond F and Kim, Baek
Journal: Journal of Biological Chemistry (2023): 104984
Functional Differentiation of Cyclins and Cyclin-Dependent Kinases in Giardia lamblia
Authors: Kim, Juri and Park, Eun-Ah and Shin, Mee Young and Park, Soon-Jung
Journal: Microbiology Spectrum (2023): e04919--22
Authors: Kim, Juri and Park, Eun-Ah and Shin, Mee Young and Park, Soon-Jung
Journal: Microbiology Spectrum (2023): e04919--22
Kukoamine A attenuates lipopolysaccharide-induced apoptosis, extracellular matrix degradation, and inflammation in nucleus pulposus cells by activating the P13K/Akt pathway
Authors: Wang, Dan and Qu, Hao and Kang, Hui and Xu, Feng and Huang, Wei and Cai, Xianhua
Journal: Bioengineered (2022): 8772--8784
Authors: Wang, Dan and Qu, Hao and Kang, Hui and Xu, Feng and Huang, Wei and Cai, Xianhua
Journal: Bioengineered (2022): 8772--8784
References
View all 49 references: Citation Explorer
Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Alexa Fluor 546-ArIB[V11L;V16A] is a potent ligand for selectively labeling alpha 7 nicotinic acetylcholine receptors
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Journal: Org Biomol Chem (2010): 4249
Neuroanatomical characteristics of acupoint "Chengshan" (BL 57) in the rat: a cholera toxin subunit B conjugated with Alexa Fluor 488 method study
Authors: Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: Zhen Ci Yan Jiu (2010): 433
Authors: Zhu XL, Bai WZ, Wu FD, Jiang J, Jing XH.
Journal: Zhen Ci Yan Jiu (2010): 433
Photoactivatable and photoconvertible fluorescent probes for protein labeling
Authors: Maurel D, Banala S, Laroche T, Johnsson K.
Journal: ACS Chem Biol (2010): 507
Authors: Maurel D, Banala S, Laroche T, Johnsson K.
Journal: ACS Chem Biol (2010): 507
Novel Alexa Fluor-488 labeled antagonist of the A(2A) adenosine receptor: Application to a fluorescence polarization-based receptor binding assay
Authors: Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: Biochem Pharmacol (2010): 506
Authors: Kecskes M, Kumar TS, Yoo L, Gao ZG, Jacobson KA.
Journal: Biochem Pharmacol (2010): 506
Application notes
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Biotin Labeling Molecules and Their Biological Applications
FAQ
How can I lyse my cells without lysing the nuclear membrane?
What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
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What are the differences between calcium ion indicators: Cal 520, Cal 520FF, and Cal 520N?
How do I make an AM ester stock solution?
Can we fix cells with glutaraldehyde and then stain with fluorescent phalloidin?
What is the difference between FluoroQuest Anti-fading Kit I and FluoroQuest Anti-fading Kit II?