iFluor® 750 amine
AAT Bioquest's iFluor® dyes are developed for labeling proteins, particularly antibodies. These dyes are optimized to have minimal fluorescence quenching effect on proteins and nucleic acids. Our iFluor® 750 dyes have fluorescence excitation and emission maxima of ~757 nm and ~779 nm, respectively, with good photostability. Our in-house comparable studies indicated that our iFluor® 750 dyes are significantly brighter than the corresponding Cy7® and Alexa Fluor® 750. These spectral characteristics make them a superior alternative to Cy7® and Alexa Fluor® 750 (Cy7® and Alexa Fluor® are the trademarks of GE Healthcare and Invitrogen). iFluor® 750 conjugates have been widely used in fluorescence animal imaging applications. The iFluor® 750 amine is stable and used for modifying carbonyl groups (e.g., aldehyde and carboxy groups).
![Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fifluor-750-amine%2Ffigure-for-ifluor-750-amine_kUqEi.jpg&w=640&q=75)
![Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fifluor-750-amine%2Ffigure-for-ifluor-750-amine_kUqEi.jpg&w=640&q=75)
![Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).](/_next/image?url=https%3A%2F%2Fimages.aatbio.com%2Fproducts%2Ffigures-and-data%2Fifluor-750-amine%2Ffigure-for-ifluor-750-amine_kUqEi.jpg&w=128&q=25)
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 750 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 95.505 µL | 477.523 µL | 955.046 µL | 4.775 mL | 9.55 mL |
5 mM | 19.101 µL | 95.505 µL | 191.009 µL | 955.046 µL | 1.91 mL |
10 mM | 9.55 µL | 47.752 µL | 95.505 µL | 477.523 µL | 955.046 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 350 amine | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 405 amine | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
iFluor® 488 amine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 amine | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 647 amine | 656 | 670 | 2500001 | 0.251 | 0.03 | 0.03 |
iFluor® 660 amine | 663 | 678 | 2500001 | 0.261 | 0.07 | 0.08 |
iFluor® 680 amine | 684 | 701 | 2200001 | 0.231 | 0.097 | 0.094 |
iFluor® 700 amine | 690 | 713 | 2200001 | 0.231 | 0.09 | 0.04 |
iFluor® 710 amine | 717 | 739 | 1900001 | 0.601 | 0.12 | 0.07 |
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Citations
View all 3 citations: Citation Explorer
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436
References
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