AAT Bioquest

iFluor® 405 amine

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 405 dyes have fluorescence excitation and emission maxima of ~403 nm and ~427 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 405 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 405 amine is stable and used for modifying carbonyl groups (e.g., aldehyde and carboxy groups).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 405 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

 0.1 mg 0.5 mg 1 mg 5 mg 10 mg 1 mM 133.568 µL 667.842 µL 1.336 mL 6.678 mL 13.357 mL 5 mM 26.714 µL 133.568 µL 267.137 µL 1.336 mL 2.671 mL 10 mM 13.357 µL 66.784 µL 133.568 µL 667.842 µL 1.336 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

 Mass (Calculate) Molecular weight Volume (Calculate) Concentration (Calculate) Moles fgpgngµgmgg / g/mol = fLpLnLµLmLL x fMpMnMµMmMM = fmolpmolnmolµmolmmolmol

Product family

 Name Excitation (nm) Emission (nm) Extinction coefficient (cm -1 M -1) Quantum yield Correction Factor (260 nm) Correction Factor (280 nm) iFluor® 350 amine 345 450 200001 0.951 0.83 0.23 iFluor® 488 amine 491 516 750001 0.91 0.21 0.11 iFluor® 555 amine 557 570 1000001 0.641 0.23 0.14 iFluor® 647 amine 656 670 2500001 0.251 0.03 0.03 iFluor® 660 amine 663 678 2500001 0.261 0.07 0.08 iFluor® 680 amine 684 701 2200001 0.231 0.097 0.094 iFluor® 700 amine 690 713 2200001 0.231 0.09 0.04 iFluor® 710 amine 716 739 1500001 0.601 0.12 0.07 iFluor® 750 amine 757 779 2750001 0.121 0.044 0.039 Show More (2)

Citations

View all 4 citations: Citation Explorer
PF-D-Trimer, a protective SARS-CoV-2 subunit vaccine: immunogenicity and application
Authors: Zhang, Zhihao and Zhou, Jinhu and Ni, Peng and Hu, Bing and Jolicoeur, Normand and Deng, Shuang and Xiao, Qian and He, Qian and Li, Gai and Xia, Yan and others,
Journal: npj Vaccines (2023): 38
Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification
Authors: Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined
Journal: Metabolomics (Los Angel) (2017): 2153--0769
Suramin inhibits cullin-RING E3 ubiquitin ligases
Authors: Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined
Journal: Proceedings of the National Academy of Sciences (2016): E2011--E2018
Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function
Authors: Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined
Journal: International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer
Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes
Authors: Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.
Journal: Mol Biosyst (2011): 1766
Visualizing dengue virus through Alexa Fluor labeling
Authors: Zhang S, Tan HC, Ooi EE.
Journal: J Vis Exp. (2011)
Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells
Authors: Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.
Journal: Biochem Biophys Res Commun (2011): 211
Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594
Authors: Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.
Journal: Zhen Ci Yan Jiu (2011): 262
Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers
Authors: Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.
Journal: J Microbiol Methods (2011): 119
Page updated on September 15, 2024

Ordering information

 Price Unit size Catalog Number 1071 Quantity

 Telephone 1-800-990-8053 Fax 1-800-609-2943 Email sales@aatbio.com International See distributors Bulk request Inquire Custom size Inquire Technical Support Contact us Purchase order Send to sales@aatbio.com Shipping Standard overnight for United States, inquire for international

748.68

DMSO

0.48

0.77

370001

403

427

0.911

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

Documents

 iFluor® A7 SE iFluor® 350 maleimide iFluor® 488 maleimide