iFluor® 405 amine

iFluor® 405 Amine is a blue-fluorescent dye with a primary amine group for covalent modification of carbonyl-containing compounds in fluorescence-based research applications.

Amine Functional Group: Enables conjugation to aldehydes and activated carboxyl groups.
Spectral Properties: Excitation at 403 nm and emission at 427 nm.
Fluorescence Performance: Bright, photostable fluorescence with minimal quenching.
Spectral Compatibility: Similar to Alexa Fluor® 405.
Applications: Suitable for fluorescent modification of carbonyl-containing molecules and biomolecular conjugates.

<p>Fluorescent dye amines are the most popular tool for conjugating dyes to a target compound with a carbonyl group (e.g., aldehyde, carboxylic acid or activated carboxy group such as NHS ester).</p>

Datasheet

SDS

Catalog	Size	Price	Quantity
1071	1 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 405 dyes have fluorescence excitation and emission maxima of ~403 nm and ~427 nm respectively. These spectral characteristics make them an excellent alternative to Alexa Fluor® 405 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 405 amine is stable and used for modifying carbonyl groups (e.g., aldehyde and carboxy groups).

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 405 amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	133.568 µL	667.842 µL	1.336 mL	6.678 mL	13.357 mL
5 mM	26.714 µL	133.568 µL	267.137 µL	1.336 mL	2.671 mL
10 mM	13.357 µL	66.784 µL	133.568 µL	667.842 µL	1.336 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	748.68 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

RAF1 activation drives epithelial cell competition through parallel mitochondrial dysfunction and GAS7-dependent protrusion formation

Authors:

Cheng, Lihong and Fujita, Hiroyuki and Imasaki, Tsuyoshi and Nitta, Ryo and Aoyama, Kazuhiro and Sakamaki, Yuriko and Nakata, Takao and Yoshihara, Shogo

PF-D-Trimer, a protective SARS-CoV-2 subunit vaccine: immunogenicity and application

Authors:

Zhang, Zhihao and Zhou, Jinhu and Ni, Peng and Hu, Bing and Jolicoeur, Normand and Deng, Shuang and Xiao, Qian and He, Qian and Li, Gai and Xia, Yan and others,

Journal:

npj Vaccines (2023): 38

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	748.68
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.48
Correction factor (280 nm)	0.77
Extinction coefficient (cm ^-1 M ^-1)	37000 ¹
Excitation (nm)	403
Emission (nm)	427
Quantum yield	0.91 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 28, 2026