iFluor® 350 succinimidyl ester

iFluor® 350 Succinimidyl Ester is a blue-fluorescent, amine-reactive dye for covalent labeling of proteins in fluorescence-based research applications.

Amine-Reactive Functionality: NHS ester selectively reacts with primary amines, including lysine residues.
Spectral Properties: Excitation at 345 nm and emission at 450 nm.
Fluorescence Performance: Bright, photostable fluorescence with minimal quenching.
Spectral Compatibility: Similar to AMCA and Alexa Fluor® 350 for UV-excited detection.
Applications: Suitable for fluorescent conjugation of antibodies and other amine-containing proteins.

<p>HeLa cells were stained with mouse anti-tubulin followed by iFluor 350 goat anti-mouse IgG (H+L).</p>

Protocol

Catalog	Size	Price
1020	1 mg	Price
71020	100 ug	Price
71500	5 mg	Price
71550	10 mg	Price

Overview

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, in particular, antibodies. These dyes are bright, photostable and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555 and 633 nm). iFluor® 350 dyes have fluorescence excitation and emission maxima of ~345nm and ~450 nm respectively. These spectral characteristics make them an excellent alternative to AMCA and Alexa Fluor® 350 labeling dye (Alexa Fluor® is the trademark of Invitrogen). iFluor® 350 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 350 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	133.36 µL	666.8 µL	1.334 mL	6.668 mL	13.336 mL
5 mM	26.672 µL	133.36 µL	266.72 µL	1.334 mL	2.667 mL
10 mM	13.336 µL	66.68 µL	133.36 µL	666.8 µL	1.334 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	749.85 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Fatty Acids and Albumin are Transported by Distinct Mechanisms in the Proximal Tubule

Authors:

Garcia, Nestor H and Gaivin, Robert J and Khan, Shenaz and Li, Vincent and Rbaibi, Youssef and Weisz, Ora A and Garvin, Jeffrey L and Schelling, Jeffrey R

Journal:

American Journal of Physiology-Renal Physiology (2025)

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

Glycosaminoglycan mimicry by COAM reduces melanoma growth through chemokine induction and function

Authors:

Piccard, Helene and Berghmans, Nele and Korpos, Eva and Dillen, Chris and Aelst, Ilse Van and Li, S and ra , undefined and Martens, Erik and Liekens, S and ra , undefined and Noppen, Sam and Damme, Jo Van and others, undefined

Journal:

International Journal of Cancer (2012): E425--E436

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	749.85
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.83
Correction factor (280 nm)	0.23
Extinction coefficient (cm ^-1 M ^-1)	20000 ¹
Excitation (nm)	345
Emission (nm)	450
Quantum yield	0.95 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 21, 2026