iFluor® 488 TCO
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. The conjugates prepared with iFluor® 488 dyes are far superior to those of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 has spectral properties similar to Alexa Fluor® 488. In some cases, it demonstrates brighter signals (Alexa Fluor® is the trademark of Invitrogen).
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 488 maleimide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 amine | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 hydrazide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 tyramide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 azide | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 alkyne | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520* | 491 | 516 | 750001 | 0.91 | 0.21 | 0.11 |
iFluor® 555 TCO | 557 | 570 | 1000001 | 0.641 | 0.23 | 0.14 |
iFluor® 594 TCO | 587 | 603 | 2000001 | 0.531 | 0.05 | 0.04 |
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Citations
View all 1 citations: Citation Explorer
CD95/Fas protects triple negative breast cancer from anti-tumor activity of NK cells
Authors: Qadir, Abdul S and Gu{\'e}gan, Jean Philippe and Ginestier, Christophe and Chaibi, Assia and Bessede, Alban and Charafe-Jauffret, Emmanuelle and Macario, Manon and Lavou{\'e}, Vincent and de la Motte Rouge, Thibault and Law, Calvin and others,
Journal: Iscience (2021): 103348
Authors: Qadir, Abdul S and Gu{\'e}gan, Jean Philippe and Ginestier, Christophe and Chaibi, Assia and Bessede, Alban and Charafe-Jauffret, Emmanuelle and Macario, Manon and Lavou{\'e}, Vincent and de la Motte Rouge, Thibault and Law, Calvin and others,
Journal: Iscience (2021): 103348
References
View all 50 references: Citation Explorer
A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Bioorthogonal Tetrazine Carbamate Cleavage by Highly Reactive trans-Cyclooctene.
Authors: van Onzen, Arthur H A M and Versteegen, Ron M and Hoeben, Freek J M and Filot, Ivo A W and Rossin, Raffaella and Zhu, Tong and Wu, Jeremy and Hudson, Peter J and Janssen, Henk M and Ten Hoeve, Wolter and Robillard, Marc S
Journal: Journal of the American Chemical Society (2020): 10955-10963
Authors: van Onzen, Arthur H A M and Versteegen, Ron M and Hoeben, Freek J M and Filot, Ivo A W and Rossin, Raffaella and Zhu, Tong and Wu, Jeremy and Hudson, Peter J and Janssen, Henk M and Ten Hoeve, Wolter and Robillard, Marc S
Journal: Journal of the American Chemical Society (2020): 10955-10963
Clickable modular polysaccharide nanoparticles for selective cell-targeting.
Authors: Peuler, Kevin and Dimmitt, Nathan and Lin, Chien-Chi
Journal: Carbohydrate polymers (2020): 115901
Authors: Peuler, Kevin and Dimmitt, Nathan and Lin, Chien-Chi
Journal: Carbohydrate polymers (2020): 115901
Facile Discovery and Quantification of Isonitrile Natural Products via Tetrazine-Based Click Reactions.
Authors: Huang, Yao-Bing and Cai, Wenlong and Del Rio Flores, Antonio and Twigg, Frederick F and Zhang, Wenjun
Journal: Analytical chemistry (2020): 599-602
Authors: Huang, Yao-Bing and Cai, Wenlong and Del Rio Flores, Antonio and Twigg, Frederick F and Zhang, Wenjun
Journal: Analytical chemistry (2020): 599-602
In Vivo Imaging of Click-Crosslinked Hydrogel Depots Following Intratympanic Injection.
Authors: Ju, Hyeon Jin and Park, Mina and Park, Ji Hoon and Shin, Gi Ru and Choi, Hak Soo and Suh, Myung-Whan and Kim, Moon Suk
Journal: Materials (Basel, Switzerland) (2020)
Authors: Ju, Hyeon Jin and Park, Mina and Park, Ji Hoon and Shin, Gi Ru and Choi, Hak Soo and Suh, Myung-Whan and Kim, Moon Suk
Journal: Materials (Basel, Switzerland) (2020)
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