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iFluor® 488 TCO

The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. AAT Bioquest offers a group of tetrazine- and TCO-containing dyes for exploring various biological systems that can use this powerful click reaction. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis. The conjugates prepared with iFluor® 488 dyes are far superior to those of fluorescein derivatives such as FITC. iFluor® 488 conjugates are significantly brighter than fluorescein conjugates and are much more photostable. Additionally, the fluorescence of iFluor® 488 is not affected by pH (4-10). This pH insensitivity is a major improvement over fluorescein, which emits its maximum fluorescence only at pH above 9. iFluor® 488 has spectral properties similar to Alexa Fluor® 488. In some cases, it demonstrates brighter signals (Alexa Fluor® is the trademark of Invitrogen).

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 488 maleimide4915167500010.910.210.11
iFluor® 488 amine4915167500010.910.210.11
iFluor® 488 hydrazide4915167500010.910.210.11
iFluor® 488 tyramide4915167500010.910.210.11
iFluor® 488 azide4915167500010.910.210.11
iFluor® 488 alkyne4915167500010.910.210.11
iFluor® 488 Styramide *Superior Replacement for Alexa Fluor 488 tyramide and Opal 520*4915167500010.910.210.11
iFluor® 555 TCO55757010000010.6410.230.14
iFluor® 594 TCO58760320000010.5310.050.04
iFluor® 647 TCO65667025000010.2510.030.03
iFluor® 488 Tetrazine4915167500010.910.210.11
iFluor®488-dUTP *1 mM in TE Buffer (pH 7.5)*4915167500010.910.210.11
ATTO 488 TCO499520900000.800.220.09
Show More (4)

Citations

View all 1 citations: Citation Explorer
CD95/Fas protects triple negative breast cancer from anti-tumor activity of NK cells
Authors: Qadir, Abdul S and Gu{\'e}gan, Jean Philippe and Ginestier, Christophe and Chaibi, Assia and Bessede, Alban and Charafe-Jauffret, Emmanuelle and Macario, Manon and Lavou{\'e}, Vincent and de la Motte Rouge, Thibault and Law, Calvin and others,
Journal: Iscience (2021): 103348

References

View all 50 references: Citation Explorer
A Systematic Study of Coumarin-Tetrazine Light-Up Probes for Bioorthogonal Fluorescence Imaging.
Authors: Galeta, Juraj and Dzijak, Rastislav and Obořil, Jan and Dračínský, Martin and Vrabel, Milan
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2020)
Bioorthogonal Tetrazine Carbamate Cleavage by Highly Reactive trans-Cyclooctene.
Authors: van Onzen, Arthur H A M and Versteegen, Ron M and Hoeben, Freek J M and Filot, Ivo A W and Rossin, Raffaella and Zhu, Tong and Wu, Jeremy and Hudson, Peter J and Janssen, Henk M and Ten Hoeve, Wolter and Robillard, Marc S
Journal: Journal of the American Chemical Society (2020): 10955-10963
Clickable modular polysaccharide nanoparticles for selective cell-targeting.
Authors: Peuler, Kevin and Dimmitt, Nathan and Lin, Chien-Chi
Journal: Carbohydrate polymers (2020): 115901
Facile Discovery and Quantification of Isonitrile Natural Products via Tetrazine-Based Click Reactions.
Authors: Huang, Yao-Bing and Cai, Wenlong and Del Rio Flores, Antonio and Twigg, Frederick F and Zhang, Wenjun
Journal: Analytical chemistry (2020): 599-602
In Vivo Imaging of Click-Crosslinked Hydrogel Depots Following Intratympanic Injection.
Authors: Ju, Hyeon Jin and Park, Mina and Park, Ji Hoon and Shin, Gi Ru and Choi, Hak Soo and Suh, Myung-Whan and Kim, Moon Suk
Journal: Materials (Basel, Switzerland) (2020)
Page updated on October 8, 2024

Ordering information

Price
Unit size
Catalog Number1005
Quantity
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Additional ordering information

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Physical properties

Molecular weight

945.16

Solvent

DMSO

Spectral properties

Correction Factor (260 nm)

0.21

Correction Factor (280 nm)

0.11

Extinction coefficient (cm -1 M -1)

750001

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.91

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.
The tetrazine-trans-cyclooctene (TCO) ligation constitutes a non-toxic biomolecule labeling method of unparalleled speed. A tetrazine-functionalized molecule reacts with a TCO-functionalized molecule, forming a stable conjugate via a dihydropyrazine moiety. This has gained popularity due to its extremely fast kinetics. iFluor® 488-TCO can be readily used to label tetrazine-modified biological molecules for fluorescence imaging and other fluorescence-based biochemical analysis.