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iFluor® 450 Goat Anti-human IgG (H+L) Antibody

Ordering information
Price ()
Catalog Number50052
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Spectral properties
Correction Factor (260 nm)0.45
Correction Factor (280 nm)0.27
Extinction coefficient (cm -1 M -1)400001
Excitation (nm)451
Emission (nm)502
Quantum yield0.821
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Other names
Host
goat
Reactivity
human
Application
Flow Cytometry (FACS), ELISA, HC, Western Blot
Correction Factor (260 nm)
0.45
Correction Factor (280 nm)
0.27
Extinction coefficient (cm -1 M -1)
400001
Excitation (nm)
451
Emission (nm)
502
Quantum yield
0.821
AAT Bioquest's anti-human secondary antibodies have well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. This iFluor® 450-labeled secondary antibody was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar iFluor® 450 goat anti-human IgG antibodies from other commercial sources, and thus can significantly increase assay sensitivities. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. This antibody was purified through affinity chromatography and conjugated to iFluor® 450 (ex/em = 451/502 nm). It is compatible with the 445 nm laser and 510/80 nm bandpass filter (for example, as in the BD FACSAria™ III).

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.45
Correction Factor (280 nm)0.27
Extinction coefficient (cm -1 M -1)400001
Excitation (nm)451
Emission (nm)502
Quantum yield0.821

Conjugation


We provide custom conjugation services for this antibody (eg. labeling of iFluor® 450 Goat Anti-human IgG (H+L) Antibody with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.