Anti-human secondary antibodies are affinity-purified polyclonal antibodies specific for human immunoglobulins (e.g., class, subclasses, or fragments). They are commonly used in the detection of a specific target to which a human primary antibody is first bound. This indirect detection scheme provides its user with a significant increase in sensitivity due to the signal amplification of multiple secondary antibodies binding to a single primary antibody. Secondary antibodies conjugated to fluorescent dyes also provide greater flexibility in multilabeling experiments. The large number of available fluorescent dyes makes it easy to design multicolor labeling panels with minimal spillover.

AAT Bioquest anti-human secondary antibodies are produced in goats and are specific for the isotype immunoglobulin G (IgG), heavy and light chains. They have been validated for use in various types of immunoassays, including ELISA, immunohistochemistry (IHC), immunoprecipitation (IP), Western blot (WB), immunoelectrophoresis (IE), fluorescence microscopy, and flow cytometry.

Goat anti-human secondary antibodies conjugated to iFluor® dyes offer exceptional brightness and photostability, outperforming other spectrally similar secondaries, such as Alexa Fluor^®-, FITC-, and Cyanine-labeled secondaries. Cross-adsorbed secondary antibodies are also available for improved specificity, greater sensitivity, and lower background in multicolor labeling experiments or in IHC experiments on samples with abundant amounts of endogenous IgG. Cross-adsorption of secondary antibodies via affinity purification eliminates cross-reactivity, improves antibody specificity, and minimizes non-specific background.