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FITC Goat Anti-human IgG (H+L) Antibody
Cross Adsorbed
AAT Bioquest's anti-human secondary antibodies have well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. This FITC-labeled secondary antibody was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar FITC goat anti-human IgG antibodies from other commercial sources, and thus can significantly increase assay sensitivities. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. This antibody was purified through affinity chromatography and conjugated to FITC (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 527/32 nm bandpass filter (for example, as in the BD FACSVerse™).
product image
product image
CatalogSize
Price
Quantity
50206200 ug
Price
502071 mg
Price
 
Antibody properties

Hostgoat
Reactivityhuman
ApplicationFlow Cytometry (FACS); ELISA; IHC; Western Blot
Spectral properties

Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)491
Emission (nm)516
Quantum yield
0.92
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
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Page updated on October 13, 2025