Name: iFluor® 488 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*
Brand: AAT Bioquest
SKU: 50058
Availability: InStock

iFluor® 488 Goat Anti-human IgG (H+L) Antibody

Cross Adsorbed

AAT Bioquest's anti-human secondary antibodies have well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. This iFluor® 488-labeled secondary antibody was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar iFluor® 488 goat anti-human IgG antibodies from other commercial sources, and thus can significantly increase assay sensitivities. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. This antibody was purified through affinity chromatography and conjugated to iFluor® 488 (ex/em = 491/516 nm). It is compatible with the 488 nm laser and 515/20 nm bandpass filter (for example, as in the BD FACSymphony™ A5).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
50058	200 ug	Price
50059	1 mg	Price

Conjugation

We provide custom conjugation services for this antibody (e.g. labeling of IgG antibody with HRP). A list of available labels can be found in the table below:

Label Type	Available Labels
AF	AF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
Proteins	HRP, Alkaline Phosphatase, Streptavidin
Tandems	APC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small Molecules	Biotin
Traditional Dyes	FITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor	350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluor	UV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page

here

Citations

View all 2 citations: Citation Explorer

Interaction between microglial cells and CD1C+ B dendritic cells leads to CD8+ T cells depletion during the early stages of renal clear cell carcinoma

Authors:

Lin, Zixuan and Wang, Liping and Niu, Yaozong and Xie, Zhaopeng and Zhao, Xiaohan

Journal:

Medicine (2024): e38691

Liensinine Inhibits Mitophagy to Potentiate Mitochondria-Targeting Artemisinin-Loaded Polymer Nanostructures for Breast Cancer Therapy

Authors:

Li, Jia-Mi and Yu, Hui and Deng, Kai and Wang, Qian and Li, Kun-Heng and Huang, Shi-Wen

Journal:

ACS Applied Nano Materials (2023)

Physical properties

Molecular weight	~150000
Solvent	Water

Antibody properties

Host	goat
Reactivity	human
Application	Flow Cytometry (FACS); ELISA; IHC; Western Blot

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 24, 2025