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Anti-Mouse Secondary Antibodies

Anti-mouse secondary antibodies are affinity-purified polyclonal antibodies specific for mouse immunoglobulins (e.g., class, subclasses, or fragments). They are commonly used in the detection of a specific target to which a mouse primary antibody is first bound. This indirect detection scheme provides its user with a significant increase in sensitivity due to the signal amplification of multiple secondary antibodies binding to a single primary antibody. Secondary antibodies conjugated to fluorescent dyes also provide greater flexibility in multilabeling experiments. The large number of available fluorescent dyes makes it easy to design multicolor labeling panels with minimal spillover.

AAT Bioquest anti-mouse secondary antibodies are produced in goats and are specific for the isotype immunoglobulin G (IgG), heavy and light chains. They have been validated for use in various types of immunoassays, including ELISA, immunohistochemistry (IHC), immunoprecipitation (IP), Western blot (WB), immunoelectrophoresis (IE), time-resolved FRET, fluorescence microscopy, and flow cytometry.

 

 

iFluor® Goat Anti-Mouse Secondaries



HeLa cells were labeled using mouse anti-tubulin antibody followed by detection with iFluor® 488 goat anti-mouse IgG (H+L). Nuclei were costained with Nuclear Blue™ DCS1.
Goat anti-mouse secondary antibodies conjugated to iFluor® dyes offer exceptional brightness and photostability, outperforming other spectrally similar secondaries, such as Alexa Fluor®-, FITC-, and Cyanine-labeled secondaries. Cross-adsorbed secondary antibodies are also available for improved specificity, greater sensitivity, and lower background in multicolor labeling experiments or in IHC experiments on samples with abundant amounts of endogenous IgG. Cross-adsorption of secondary antibodies via affinity purification eliminates cross-reactivity, improves antibody specificity, and minimizes non-specific background.

Available Goat Anti-Mouse Antibodies


 

Table 1. Anti-mouse secondary antibodies

Product
Host
Target Species
Conjugate
Ex (nm)
Em (nm)
Spectrum
Application
Size
Cat No.
Biotinylated goat anti-mouse IgG (H+L)GoatMouseBiotin   WB, IHC/ICC/IF, ELISA, Flow1 mg16729
Goat anti-mouse IgG (H+L) AgaroseGoatMouseAgarose   IP, AP10 mg55010
HRP-labeled goat anti-mouse IgG (H+L)GoatMouseHRP   WB, IHC/ICC/IF, ELISA, IE1 mg16728
MegaWox™ PolyHRP-goat anti-mouse IgGGoatMousepolyHRP   WB, IHC/ICC/IF, ELISA, IE1 mg16728
iFluor® 350 goat anti-mouse IgG (H+L)GoatMouseiFluor® 350345450IHC/ICC/IF, Flow200 µg16440
iFluor® 350 goat anti-mouse IgG (H+L)GoatMouseiFluor® 350345450IHC/ICC/IF, Flow1 mg16730
iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*GoatMouseiFluor® 350345450IHC/ICC/IF, Flow200 µg16520
iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*GoatMouseiFluor® 350345450IHC/ICC/IF, Flow1 mg16770
iFluor® 405 goat anti-mouse IgG (H+L)GoatMouseiFluor® 405403427IHC/ICC/IF, Flow200 µg16444
iFluor® 405 goat anti-mouse IgG (H+L)GoatMouseiFluor® 405403427IHC/ICC/IF, Flow1 mg16731