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Nuclear Blue™ DCS1 *5 mM DMSO Solution*

Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Blue™ DCS1 (Cat#17548, Blue).
Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Blue™ DCS1 (Cat#17548, Blue).
Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Blue™ DCS1 (Cat#17548, Blue).
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using EpCAM Rabbit mAb followed by iFluor® 555 goat anti-rabbit IgG (H+L) (Cat No. 16620, red). The nuclei were counterstained using Nuclear Blue™ DCS1 (Cat No. 17548, blue).
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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weightN/A
SolventDMSO
Spectral properties
Excitation (nm)348
Emission (nm)469
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


Molecular weight
N/A
Excitation (nm)
348
Emission (nm)
469
Our Nuclear Blue™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells. The Nuclear Blue™ DCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead, fixed or apoptotic cells with the DAPI filter sets. For example, Nuclear Blue™ DCS1 can be used with GFP cell lines.

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Spectral properties

Excitation (nm)348
Emission (nm)469

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Citations


View all 3 citations: Citation Explorer
New strategy for promoting vascularization in tumor spheroids in a microfluidic assay
Authors: Wan, Zhengpeng and Floryan, Marie A and Coughlin, Mark F and Zhang, Shun and Zhong, Amy X and Shelton, Sarah E and Wang, Xun and Xu, Chenguang and Barbie, David A and Kamm, Roger D
Journal: Advanced Healthcare Materials (2022): 2201784
One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1

References


View all 65 references: Citation Explorer
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Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
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Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534
Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887
DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
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Journal: Am J Clin Pathol (2008): 706
Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
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Journal: Cytometry A (2008): 555
Addition of gold nanoparticles to real-time PCR: effect on PCR profile and SYBR Green I fluorescence
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Journal: Anal Bioanal Chem (2008): 887
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