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Nuclear Blue™ DCS1 *5 mM DMSO Solution*

Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Blue™ DCS1 (Cat#17548, Blue).
Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Blue™ DCS1 (Cat#17548, Blue).
Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Blue™ DCS1 (Cat#17548, Blue).
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using EpCAM Rabbit mAb followed by iFluor® 555 goat anti-rabbit IgG (H+L) (Cat No. 16620, red). The nuclei were counterstained using Nuclear Blue™ DCS1 (Cat No. 17548, blue).
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Physical properties
Molecular weightN/A
Spectral properties
Excitation (nm)348
Emission (nm)469
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Our Nuclear Blue™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells. The Nuclear Blue™ DCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead, fixed or apoptotic cells with the DAPI filter sets. For example, Nuclear Blue™ DCS1 can be used with GFP cell lines.


Fluorescence microscope

ExcitationDAPI Filter
EmissionDAPI Filter
Recommended plateBlack wall/clear bottom

Example protocol


Spectral Properties

Ex/Em = 355/458 nm (bound to DNA) 


Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types, and factors may influence staining. Residual detergent on glassware may also affect the staining of many organisms and cause brightly stained material to appear in solutions with or without cells present.

  1. Add Nuclear Blue™ DCS1 (2 to10 µM) into the fixed, dead or apoptotic cells (either suspension or adherent) and incubate the cells for 15 to 60 minutes.

    Note: In initial experiments, it is advisable to test a wide range of dye concentrations in order to determine the optimal concentration that yields the desired result.

  2. Wash the cells twice with Hanks and 20 mM HEPES buffer (HBSS) or a buffer of your choice. Then fill the wells with fresh HBSS or growth medium.

  3. Observe the cells using a fluorescence microscope, fluorescence microplate reader, or flow cytometer equipped with the desired filter set. 


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Spectral properties

Excitation (nm)348
Emission (nm)469



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In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751
Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93
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Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759
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Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
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Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555
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Authors: Allan RW, Ansari-Lari MA, Jordan S.
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Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887
Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534
Group testing to annihilate pairs applied to DNA cross-hybridization elimination using SYBR Green I
Authors: Bishop M, Macula AJ, Nimmo K, Wood L, Pogozelski WK, Renz TE.
Journal: J Comput Biol (2007): 84
DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies
Authors: Swerts K, Van Roy N, Benoit Y, Laureys G, Philippe J.
Journal: Clin Chim Acta (2007): 154