Nuclear Blue™ DCS1 5 mM DMSO Solution

Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Blue™ DCS1 (Cat#17548, Blue).

Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using EpCAM Rabbit mAb followed by iFluor® 555 goat anti-rabbit IgG (H+L) (Cat No. 16620, red). The nuclei were counterstained using Nuclear Blue™ DCS1 (Cat No. 17548, blue).

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	348
Emission (nm)	469

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Alternative formats

Nuclear Green™ DCS1 *5 mM DMSO Solution*

Nuclear Orange™ DCS1 *5 mM DMSO Solution*

Nuclear Red™ DCS1 *5 mM DMSO Solution*

Overview SDS Protocol

Molecular weight

N/A

Excitation (nm)

348

Emission (nm)

469

Our Nuclear Blue™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells. The Nuclear Blue™ DCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead, fixed or apoptotic cells with the DAPI filter sets. For example, Nuclear Blue™ DCS1 can be used with GFP cell lines.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Excitation (nm)	348
Emission (nm)	469

Product Family

Name	Excitation (nm)	Emission (nm)
Nuclear Green™ DCS1 5 mM DMSO Solution	503	527
Nuclear Orange™ DCS1 5 mM DMSO Solution	514	556
Nuclear Red™ DCS1 5 mM DMSO Solution	631	651

Images

Figure 1. Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Blue™ DCS1 (Cat#17548, Blue).

Figure 2. Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using EpCAM Rabbit mAb followed by iFluor® 555 goat anti-rabbit IgG (H+L) (Cat No. 16620, red). The nuclei were counterstained using Nuclear Blue™ DCS1 (Cat No. 17548, blue).

Citations

View all 3 citations: Citation Explorer

New strategy for promoting vascularization in tumor spheroids in a microfluidic assay
Authors: Wan, Zhengpeng and Floryan, Marie A and Coughlin, Mark F and Zhang, Shun and Zhong, Amy X and Shelton, Sarah E and Wang, Xun and Xu, Chenguang and Barbie, David A and Kamm, Roger D
Journal: Advanced Healthcare Materials (2022): 2201784

One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124

Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1

References

View all 65 references: Citation Explorer

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Journal: J Antimicrob Chemother (2009): 751

Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
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Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534

Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887

DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
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Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
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