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Nuclear Blue™ DCS1 *5 mM DMSO Solution*

Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Blue™ DCS1 (Cat#17548, Blue).
Fluorescence image of HeLa cells fixed with 4% formaldehyde then stained with iFluor 488 Phalloidin (Cat#23115, Green) and nuclei stain Nuclear Blue™ DCS1 (Cat#17548, Blue).
Immunohistochemical analysis of paraffin-embedded human lung adenocarcinoma using EpCAM Rabbit mAb followed by iFluor® 555 goat anti-rabbit IgG (H+L) (Cat No. 16620, red). The nuclei were counterstained using Nuclear Blue™ DCS1 (Cat No. 17548, blue).
Ordering information
Price ()
Catalog Number17548
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventDMSO
Spectral properties
Excitation (nm)348
Emission (nm)469
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


Excitation (nm)
348
Emission (nm)
469
Our Nuclear Blue™ DCS1 is a fluorogenic, DNA-selective and cell-impermeant dye for analyzing DNA content in dead, fixed or apoptotic cells. The Nuclear Blue™ DCS1 has its blue fluorescence significantly enhanced upon binding to DNA. It can be used in fluorescence imaging, microplate and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead, fixed or apoptotic cells with the DAPI filter sets. For example, Nuclear Blue™ DCS1 can be used with GFP cell lines.

Spectrum


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Spectral properties

Excitation (nm)348
Emission (nm)469

Citations


View all 2 citations: Citation Explorer
One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1

References


View all 65 references: Citation Explorer
In vitro activity of pyrvinium pamoate against Entamoeba histolytica and Giardia intestinalis using radiolabelled thymidine incorporation and an SYBR Green I-based fluorescence assay
Authors: Downey AS, Graczyk TK, Sullivan DJ.
Journal: J Antimicrob Chemother (2009): 751
Detection of methylation in hepatocellular carcinoma using SYBR Green fluorescent quantitative PCR
Authors: Yang B, Lou C, Gao Y, Du Z, Song W.
Journal: Zhonghua Yi Xue Yi Chuan Xue Za Zhi (2008): 534
Estimation of copy number using SYBR Green: confounding by AT-rich DNA and by variation in amplicon length
Authors: Colborn JM, Byrd BD, Koita OA, Krogstad DJ.
Journal: Am J Trop Med Hyg (2008): 887
DRAQ5-based, no-lyse, no-wash bone marrow aspirate evaluation by flow cytometry
Authors: Allan RW, Ansari-Lari MA, Jordan S.
Journal: Am J Clin Pathol (2008): 706
Interaction of a DNA intercalator DRAQ5, and a minor groove binder SYTO17, with chromatin in live cells--influence on chromatin organization and histone-DNA interactions
Authors: Wojcik K, Dobrucki JW.
Journal: Cytometry A (2008): 555
Addition of gold nanoparticles to real-time PCR: effect on PCR profile and SYBR Green I fluorescence
Authors: Haber AL, Griffiths KR, Jamting AK, Emslie KR.
Journal: Anal Bioanal Chem (2008): 887
Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I
Authors: Shaik GM, Draberova L, Draber P, Boubelik M.
Journal: Nucleic Acids Res (2008): e93
Highly sensitive and selective detection of Hg(2+) in aqueous solution with mercury-specific DNA and Sybr Green I
Authors: Wang J, Liu B.
Journal: Chem Commun (Camb) (2008): 4759
Group testing to annihilate pairs applied to DNA cross-hybridization elimination using SYBR Green I
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Journal: J Comput Biol (2007): 84
DRAQ5: improved flow cytometric DNA content analysis and minimal residual disease detection in childhood malignancies
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Journal: Clin Chim Acta (2007): 154