Name: Nuclear Green™ Photo-Fixable DCS1 *5 mM DMSO Solution*
Brand: AAT Bioquest
SKU: 17570
Price: 301 USD
Availability: InStock

Nuclear Green™ Photo-Fixable DCS1

5 mM DMSO Solution

Nuclear Green™ Photo-Fixable DCS1 is a unique fluorogenic, DNA-selective, and cell-impermeant dye for analyzing DNA content in dead, fixed, or apoptotic cells. It selectively stains cell membrane-compromised cells. It contains a DNA-binding fluorophore, thus readily binds to DNA upon interaction. Nuclear Green™ Photo-Fixable DCS1 has its green fluorescence significantly enhanced upon binding to DNA. In addition to its DNA-binding fluorophore, it contains a secondary DNA-photoreactive group. When needed, a moderate UV illumination (e.g., 350 nm) makes the DNA-bound dye readily react with DNA and makes the dye permanently conjugate with the DNA. It is optimized for surviving the cell fixation process better than common DNA dyes such as PI, DAPI, Hoechst, 7-AAD, DRAQ-5, DRAQ-7, or SYBR Green. It can be used in fluorescence imaging, microplate, and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead, fixed, or apoptotic cells with proper filter sets.

Live, apoptotic (Staurosporine treated cells), and dead (4% formaldehyde-treated) HeLa cells were stained with Nuclear Green™ Photo-Fixable DCS1 dye and imaged using a fluorescence microscope equipped with a FITC filter set. The images were captured before and after fixation with a 365 nm Transilluminator for 20 minutes.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17570	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Nuclear Green™ Photo-Fixable DCS1 *5 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	101.183 µL	505.914 µL	1.012 mL	5.059 mL	10.118 mL
5 mM	20.237 µL	101.183 µL	202.366 µL	1.012 mL	2.024 mL
10 mM	10.118 µL	50.591 µL	101.183 µL	505.914 µL	1.012 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	988.31 g/mol	=	mL	x	mM	=	mmol

Physical properties

Molecular weight	988.31
Solvent	DMSO

Spectral properties

Excitation (nm)	502
Emission (nm)	527

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on December 28, 2025