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Nuclear Green™ Photo-Fixable DCS1
5 mM DMSO Solution
Nuclear Green™ Photo-Fixable DCS1 is a unique fluorogenic, DNA-selective, and cell-impermeant dye for analyzing DNA content in dead, fixed, or apoptotic cells. It selectively stains cell membrane-compromised cells. It contains a DNA-binding fluorophore, thus readily binds to DNA upon interaction. Nuclear Green™ Photo-Fixable DCS1 has its green fluorescence significantly enhanced upon binding to DNA. In addition to its DNA-binding fluorophore, it contains a secondary DNA-photoreactive group. When needed, a moderate UV illumination (e.g., 350 nm) makes the DNA-bound dye readily react with DNA and makes the dye permanently conjugate with the DNA. It is optimized for surviving the cell fixation process better than common DNA dyes such as PI, DAPI, Hoechst, 7-AAD, DRAQ-5, DRAQ-7, or SYBR Green. It can be used in fluorescence imaging, microplate, and flow cytometry applications. This DNA-binding dye might be used for multicolor analysis of dead, fixed, or apoptotic cells with proper filter sets.
Live, apoptotic (Staurosporine treated cells), and dead (4% formaldehyde-treated) HeLa cells were stained with Nuclear Green™ Photo-Fixable DCS1 dye and imaged using a fluorescence microscope equipped with a FITC filter set. The images were captured before and after fixation with a 365 nm Transilluminator for 20 minutes.
Live, apoptotic (Staurosporine treated cells), and dead (4% formaldehyde-treated) HeLa cells were stained with Nuclear Green™ Photo-Fixable DCS1 dye and imaged using a fluorescence microscope equipped with a FITC filter set. The images were captured before and after fixation with a 365 nm Transilluminator for 20 minutes.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
175701 mg
Price
 
Physical properties
Molecular weight988.31
SolventDMSO
Spectral properties
Excitation (nm)502
Emission (nm)527
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 28, 2025