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XFD350 Goat Anti-human IgG (H+L) Antibody *XFD350 Same Structure to Alexa Fluor™ 350*

Ordering information
Price ()
Catalog Number50152
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Spectral properties
Correction Factor (260 nm)0.25
Correction Factor (280 nm)0.19
Extinction coefficient (cm -1 M -1)19000
Excitation (nm)343
Emission (nm)441
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure

OverviewpdfSDSpdfProtocol


Other names
Host
goat
Reactivity
human
Application
Flow Cytometry (FACS), ELISA, HC, Western Blot
Correction Factor (260 nm)
0.25
Correction Factor (280 nm)
0.19
Extinction coefficient (cm -1 M -1)
19000
Excitation (nm)
343
Emission (nm)
441
AAT Bioquest's anti-human secondary antibodies have well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. This Alexa Fluor® 350-labeled secondary antibody was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar Alexa Fluor® 350 goat anti-human IgG antibodies from other commercial sources, and thus can significantly increase assay sensitivities. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. This antibody was purified through affinity chromatography and conjugated to XFD350 (ex/em = 343/441 nm). XFD350 is manufactured by AAT Bioquest, and it has the same chemical structure of Alexa Fluor® 350 (Alexa Fluor® is the trademark of ThermoFisher). It is compatible with the 355 nm laser and 450/50 nm bandpass filter (for example, as in the BD LSRFortessa™ Cell Analyzer).

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.25
Correction Factor (280 nm)0.19
Extinction coefficient (cm -1 M -1)19000
Excitation (nm)343
Emission (nm)441

Conjugation


We provide custom conjugation services for this antibody (eg. labeling of XFD350 Goat Anti-human IgG (H+L) Antibody *XFD350 Same Structure to Alexa Fluor™ 350* with HRP). A list of available labels can be found in the table below:

AFAF350, AF488, AF555, AF594, AF647, AF680, AF700, AF750
ProteinsHRP, Alkaline Phosphatase, Streptavidin
TandemsAPC, APC/Cy7, APC/AF750, APC/iFluor™ 700, APC/iFluor™ 750, PE, PE/Cy5, PE/Cy7, PE/AF610, PE/AF700, PE/iFluor™ 594, PE/iFluor™ 647, PE/iFluor™ 700, PE/iFluor™ 750, PE/Texas Red®, PerCP, PerCP/Cy5.5
Small MoleculesBiotin
Traditional DyesFITC (fluorescein), TRITC, PacBlue, PacOrange, Cy3, Cy5
iFluor350, 405, 430, 450, 488, 514, 532, 546, 555, 560, 568, 594, 610, 633, 647, 660, 670, 680, 700, 710, 750, 790, 800, 810, 820, 840, 860, A7
mFluorUV375, UV460, Violet 450, Violet 500, Violet 510, Violet 540, Blue 570, Green 620, Red 700, Red 780

For additional information about custom conjugations, please consult our services page here.