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Western Blotting Assays

Western blotting is an analytical technique used to detect the presence of specific proteins in a complex biological sample. This process involves the electrophoretic separation of proteins, the transfer of separated proteins from a gel to a stable membrane substrate, and their subsequent detection by antibodies labeled with reporter molecules. Antibodies are selected based on their specificity for the protein of interest, and this specificity of the antibody-protein interaction enables target identification. Common reporter molecules include enzymes, such as horseradish peroxidase (HRP) and alkaline phosphatase (ALP), or fluorescent dyes, such as iFluor® 488, with the latter affording greater sensitivity and multiplexing capacity.

Western blotting is used extensively in biochemistry applications targeting complex proteins, testing for disease markers (e.g., HIV, Lyme disease, or Hepatitis B), and confirming protein production in cloning experiments. With the development of novel blotting technologies and specialized automated equipment, western blotting has become a cornerstone in proteomics research and protein detection, producing qualitative and semi-quantitative results.

 

 

Western Blotting Workflow


Electrophoretic Separation of Proteins

ProLite™ Orange Gel Stain
Three-fold dilution series of BSA standards were separated on a NuPAGE® 4-12% Bis-Tris gel and stained with A) ProLite™ Orange Protein Gel Stain or B) Coomassie brilliant blue (CBB) according to standard protocols. The ProLite™ Orange stained gels were photographed using a SYPRO Orange filter. The CBB-stained gels were photographed using transmitted white light without an optical filter. Lane 1: 15µg, Lane 7: ~20ng, Lane 10: ~0.8 ng BSA.

Like many characterization techniques, western blotting uses gel electrophoresis to separate proteins based on size and charge. In this method, an electric field forces proteins through the pores of a gel. Smaller proteins migrate faster than larger ones due to less resistance from the gel until pockets within the gel encapsulating proteins of similar size and charge are produced. Gels typically have multiple channels or wells that separate several related experimental mixtures. For accurate size determination, a protein standard is run in conjunction with the sample. Standards include several proteins ranging in size from 10 to 250 kDa for comparison purposes and are available in various formats. The PageTell™ Prestained 10 to 250 kDa Protein Ladder (Cat No. 60080 and 60081) is a three-color protein standard that includes 13-prestained proteins ranging from 10 to 250 kDa in size. Eleven of the proteins are covalently coupled with a blue chromophore except for two reference bands, a green and a red band at 25 kDa and 75 kDa, respectively, and when separated form distinctly visible bands. PageTell™ protein standards can be conveniently loaded directly onto gels without any preparation (e.g., heat or additional loading buffer).

Since proteins are complex macromolecules folded in on themselves, western blotting often uses a procedure such as PAGE (polyacrylamide gel electrophoresis) to provide information about its mass, charge, purity, and presence. There are several forms of PAGE, such as non-denaturing PAGE or native PAGE, which separate proteins based on their mass-to-charge ratios, but the most common is SDS-PAGE. In SDS-PAGE, the detergent sodium dodecyl sulfate is used to denature proteins and normalize their mass-to-charge ratios. The subsequent application of heat is used to remove the secondary and tertiary protein structure points so that each protein presents as one long linear macromolecule. In addition, SDS applies a negative charge equally across the length of the protein so that each molecule's movement through the gel, as driven by the electric field, is determined by its molecular weight only. Once separated by electrophoresis, proteins can be visualized in a gel using various stains, such as ProLite™ Orange (Cat No. 18000), or transferred onto a membrane for western blot analysis.


Table 1. Specifications for fluorescent protein gel stains.

Stain
Ex/Em (nm)
Detection Range
Stain Time
Applications
Fixatives Requried
De-staining Required
Cat No.
ProLite™ Orange484/586 nm0.8 ng to 15 µg∼1 hour1D SDS-PAGENoNo18000 (100 µL)
18001 (1 mL)
 

Transferring Proteins to the Membrane

Once the mixture of proteins has been separated, the pattern is transferred onto a membrane for detection. Western blot membranes are either nitrocellulose (NC) or polyvinylidene difluoride (PVDF), which have a high affinity for proteins. Most often, the separated pockets of protein are transferred onto the membrane using electroblotting or electrophoretic transfer; this method pulls the negatively charged molecules through the cross-section of the gel and onto the membrane, where they bind to the surface. When transferred to the membrane, the separated pockets of molecular weight maintain their physical position.


Table 2. Comparison of nitrocellulose and polyvinylidene fluoride membranes.

Nitrocellulose (NC)
Polyvinylidene Fluoride (PVDF)
Protein binding capacity80 to 100 µg/cm²170 to 200 µg/cm²
Binding interactionsProteins bind to membrane through hydrophobic interactionsProteins bind to membrane through hydrophobic and dipole interactions
Physical characteristicsBrittle, fragile and not chemically resistantPhysically durable and chemically resistant
Pore size0.1, 0.2 or 0.45µm0.1, 0.2 or 0.45µm
Membrane formatPre-wetted with methanolNo need to pre-wet
Attributes
  • Ideal for detecting low molecular weight proteins
  • Comparatively low sensitivity
  • Generates lower background interference
  • Difficult to stripe and probe
  • Used for amino acid analysis and dot/slot blotting
  • Ideal for detecting high molecular weight proteins
  • High sensitivity
  • Generates higher background interference
  • Ideal for reprobing and sequencing
  • Used for protein sequencing, amino acid analysis, and solid phase assay systems


Blocking nonspecific sites

After transfer, it is critical to block any remaining sites on the surface of the membrane to prevent the nonspecific binding of detection antibodies during subsequent steps. Various buffers, including skim milk, bovine serum albumin (BSA), and highly purified proteins, have been successfully used as blocking buffers in western blotting. Consequently, determining the appropriate blocking buffer is system-dependent. Since the efficacy of blocking agents can vary between applications, empirically testing various blocking buffers can help achieve optimal results. Nevertheless, the blocking buffer should improve the sensitivity of the assay by reducing background interference and improving the signal-to-noise ratio.


Table 3. Comparison of blocking buffers for western blotting.

Blocking Buffer
Benefits
Considerations
Skim milk
  • Inexpensive
  • Contains multiple types of proteins
Contains biotin and phosphoproteins, which can interfere with streptavidin-biotin detection strategies and detection of phosphorylated target proteins. Due to the number of proteins within milk, milk may mask some antigens and lower the detection limit of the western blot.
Bovine serum albumin (BSA)
  • Good alternative to milk
  • Can be used in biotin-streptavidin systems or when probing for phosphoproteins
Various grades of BSA are commercially available that can impact signal-to-noise. BSA is generally a weaker blocker, which can result in more non-specific antibody binding, but can increase the detection sensitivity for low-abundant proteins.
Purified proteins
  • Single-protein blocking buffers can provide fewer chances of cross-reaction with assay components than serum or milk solutions.
  • Ideal when blockers, such as non-fat milk, block antigen-antibody binding
More expensive than traditional non-fat milk formulations.


Detecting Proteins Using Primary and Secondary Antibodies

In the final steps of the process, the blocked membrane is probed with primary antibodies specific to the protein or epitope of interest. In most cases, primary antibodies are unlabeled, and tagged secondary antibodies are used to bind to the primary antibodies and assist in detecting the target antigen. This type of strategy, called indirect detection (figure 2), offers several advantages, including signal amplification, enhanced sensitivity, and flexibility. However, additional incubation and wash steps are required. The choice of secondary antibody will depend on either the host species of the primary antibody or any hapten or other tag (e.g., histidine (His-tag) or hemagglutinin (HA-tag)) bound to the primary antibody. For example, if the primary antibody is a mouse monoclonal, an anti-mouse secondary antibody is required from a non-mouse host. Once antibodies have been attached, the membrane is ready for review.

Representation of direct and indirect detection.

Representation of direct and indirect detection. Indirect detection affords the binding of multiple secondary antibodies to each primary antibody resulting in significant signal amplification (created with BioRender.com).

For multiplex western blot analysis, highly cross-adsorbed secondary antibodies are recommended to minimize cross-reactivity between antibodies. These antibodies are manufactured with an additional purification step to remove antibodies that bind to off-target immunoglobulin (IgG) species.

 

 

Western Blotting Detection Methods


Detection methods for visualizing a western blot can be colorimetric, fluorescent, chemiluminescent, or radioactive. Radioactive markers were used in the past but are no longer favored due to the expense and special handling required. Nowadays, secondary antibodies tagged with fluorophores or enzymes (e.g., HRP) are used to detect proteins of interest.


Enzymatic Labels

Horseradish peroxidase (HRP) and alkaline phosphatase (AP) are the two enzymes most commonly used for protein detection. Enzyme-conjugates offer the most flexibility in detection as an array of chromogenic, fluorogenic, and chemiluminescent substrates are available for use with either enzyme. Of the three, chromogenic substrates are the simplest to use, cost-effective, and provide direct visualization of signal development (i.e., enzyme-substrate reaction results in colored precipitate). Chemiluminescent substrates differ from other substrates in that the enzyme-substrate reaction produces light as a byproduct. In well-optimized assays, chemiluminescent reactions can produce a stable output of light for several hours that can be detected using X-ray film or digital imaging equipment, such as a charged-coupled device (CCD). Compared to the other enzymatic methods, chemiluminescence detection is the most widely used and offers the greatest sensitivity, providing pictogram to femtogram-level detection.

When a higher degree of signal amplification and detection sensitivity is needed, poly-HRP secondary antibodies can be used instead of traditional HRP conjugates. The HRP heteropolymer core conjugated to secondary antibodies drastically increases the molar ratio on the secondary antibody without affecting its functionality. This allows more HRP molecules to be available at the immune complex to react with the substrates developing more desirable signals.




Table 5. Colorimetric, fluorimetric and chemiluminescent HRP Substrates.

Substrate
Detection
Absorbance (nm)
Ex (nm)
Em (nm)
Unit size
Cat No.
ABTSColorimetric420 nm--100 mL
1 L
11013
11001
TMBColorimetric450 nm / Yellow
650 nm / Blue
--100 mL
1 L
11012
11003
Amplite® BlueFluorimetric-324 nm409 nm25 mg11005
Amplite® ADHPFluorimetric-570 nm583 nm25 mg11000
Amplite® RedFluorimetric-570 nm583 nm1000 Assays11011
Amplite® IRFluorimetric-646 nm667 nm1 mg11009
LuminolChemiluminescent--410 nm1 mg11050


Fluorescent Labels

Multiplex Western Blot Stain
P53 Antibody Western Blot, validation (1:1000 dilution) in T8.

While enzyme-labeled secondary antibodies can be used to detect proteins fluorescently, the use of fluorophore-labeled secondary antibodies is preferred since a substrate development step is not required in the assay. More importantly, fluorophore-labeled secondary antibodies are easy-to-use, quantitative, provide wider ranges of detection and linearity, and have the advantage of multiplexing (i.e., detection of multiple target proteins on a single blot). Secondary antibodies can be conjugated to iFluor® dyes, Alexa Fluor® dyes, and traditional dyes such as fluoresceins, rhodamines, and cyanines, to generate a detectable single. The target protein is visualized by exciting the fluorophores using an imaging system, such as a ChemiDoc or Odyssey® imaging system, equipped with an appropriate light source and filter. As the fluorophore absorbs light and transcends to a higher energy state, vibrational effects cause it to relax back to its ground state and emit a photon of light at a longer wavelength. Imaging systems typically use a combination of laser and filter sets, in some cases up to 18 channels, to support dozens of fluorophore pairings.

iFluor® conjugated secondary antibodies exhibit superior brightness and photostability, outperforming Alexa Fluor® and other spectrally similar secondary antibody conjugates. These iFluor® secondary antibodies recognize IgG heavy chains and all classes of immunoglobulin light chains from either mice, rabbits, or humans. For multiplex western blot analysis, obtain superior images with highly cross-adsorbed iFluor® near-infrared fluorescent dye-labeled secondary antibodies. These antibodies have been manufactured with an additional purification step that helps eliminate species cross-reactivity and increases specificity, and their near-infrared fluorescence minimizes cross-talk.

 

iFluor® Secondary Antibody Product Guide


ParameterDefinition
Detection StrategyIt uses indirect detection strategies whereby the secondary antibody detects an unlabeled primary antibody bound to the target of interest.
ApplicationsDesigned for use in various cell analysis and protein analysis applications, including cell imaging (e.g., IHC/ICC/IF), fluorescent ELISA, and fluorescent western blotting.
Product Attributes
  • Enhanced sensitivity - multiple iFluor® secondary antibodies can bind to a single primary antibody resulting in signal amplification and increased assay sensitivity.
  • pH Insensitivity - iFluor® dyes emit bright fluorescence intensities over a wide range (pH 3 to 11).
  • Excellent Water Solubility - eliminates the precipitation and aggregation of iFluor® secondary antibodies.
  • Increased flexibility when designing multi-color fluorescent western blot panels.
Targets AvailableHost
  • Goat
Species
Fluorophores Available

 

Product Ordering Information


 

Table 6. Product ordering information for secondary immunoreagents.

Cat#
Product Name
Ex
Em
Unit Size
11035MegaWox™ polyHRP-Goat Anti-Mouse IgG Conjugate  1 mg
11037MegaWox™ polyHRP-Goat Anti-Rabbit IgG Conjugate  1 mg
11540Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*5715851000 Tests
11541Amplite® Fluorimetric Goat Anti-Rabbit IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*5715851000 Tests
16380XFD350 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*3464451 mg
16383XFD488 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*4945171 mg
16388XFD594 goat anti-mouse IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*5906171 mg
16395XFD350 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*3464451 mg
16398XFD488 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*4945171 mg
16404XFD594 goat anti-rabbit IgG (H+L) *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*5906171 mg
16440iFluor® 350 goat anti-mouse IgG (H+L)345442200 ug
16444iFluor® 405 goat anti-mouse IgG (H+L)401420200 ug
16448iFluor® 488 goat anti-mouse IgG (H+L)491514200 ug
16452iFluor® 514 goat anti-mouse IgG (H+L)511527200 ug
16456iFluor® 532 goat anti-mouse IgG (H+L)536560200 ug
16457iFluor® 546 goat anti-mouse IgG (H+L)541557200 ug
16460iFluor® 555 goat anti-mouse IgG (H+L)552567200 ug
16462iFluor® 568 goat anti-mouse IgG (H+L)568587200 ug
16468iFluor® 594 goat anti-mouse IgG (H+L)592614200 ug
16470iFluor® 568 goat anti-mouse IgG (H+L)5685871 mg
16478iFluor® 633 goat anti-mouse IgG (H+L)638655200 ug
16482iFluor® 647 goat anti-mouse IgG (H+L)654674200 ug
16486iFluor® 680 goat anti-mouse IgG (H+L)682701200 ug
16494iFluor® 700 goat anti-mouse IgG (H+L)693713200 ug
16506iFluor® 750 goat anti-mouse IgG (H+L)753779200 ug
16507iFluor® 790 goat anti-mouse IgG (H+L)782811200 ug
16518trFluor™ Eu goat anti-mouse IgG (H+L)346617100 ug
16519trFluor™ Tb goat anti-mouse IgG (H+L)330544100 ug
16520iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*345442200 ug
16524iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*401420200 ug
16528iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*491514200 ug
16532iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*511527200 ug
16536iFluor® 532 goat anti-mouse IgG (H+L) *Cross Adsorbed*536560200 ug
16537iFluor® 546 goat anti-mouse IgG (H+L) *Cross Adsorbed*541557200 ug
16540iFluor® 555 goat anti-mouse IgG (H+L) *Cross Adsorbed*552567200 ug
16541iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*568587200 ug
16548iFluor® 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*592614200 ug
16558iFluor® 633 goat anti-mouse IgG (H+L) *Cross Adsorbed*638655200 ug
16562iFluor® 647 goat anti-mouse IgG (H+L) *Cross Adsorbed*654674200 ug
16566iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*682701200 ug
16574iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*693713200 ug
16586iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*753779200 ug
16587iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*782811200 ug
16598trFluor™ Eu goat anti-mouse IgG (H+L) *Cross Adsorbed*346617100 ug
16599trFluor™ Tb goat anti-mouse IgG (H+L) *Cross Adsorbed*330544100 ug
16600iFluor® 350 goat anti-rabbit IgG (H+L)345442200 ug
16604iFluor® 405 goat anti-rabbit IgG (H+L)401420200 ug
16608iFluor® 488 goat anti-rabbit IgG (H+L)491514200 ug
16612iFluor® 514 goat anti-rabbit IgG (H+L)511527200 ug
16616iFluor® 532 goat anti-rabbit IgG (H+L)536560200 ug
16618iFluor® 546 goat anti-rabbit IgG (H+L)541557200 ug
16620iFluor® 555 goat anti-rabbit IgG (H+L)552567200 ug
16622iFluor® 568 goat anti-rabbit IgG (H+L)568587200 ug
16628iFluor® 594 goat anti-rabbit IgG (H+L)592614200 ug
16638iFluor® 633 goat anti-rabbit IgG (H+L)638655200 ug
16642iFluor® 647 goat anti-rabbit IgG (H+L)654674200 ug
16646iFluor® 680 goat anti-rabbit IgG (H+L)682701200 ug
16652iFluor® 700 goat anti-rabbit IgG (H+L)693713200 ug
16660iFluor® 750 goat anti-rabbit IgG (H+L)753779200 ug
16661iFluor® 790 goat anti-rabbit IgG (H+L)782811200 ug
16668trFluor™ Eu goat anti-rabbit IgG (H+L)346617100 ug
16669trFluor™ Tb goat anti-rabbit IgG (H+L)330544100 ug
16670iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*345442200 ug
16674iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*401420200 ug
16678iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*491514200 ug
16682iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*511527200 ug
16686iFluor® 532 goat anti-rabbit IgG (H+L) *Cross Adsorbed*536560200 ug
16688iFluor® 546 goat anti-rabbit IgG (H+L) *Cross Adsorbed*541557200 ug
16690iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*552567200 ug
16692iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*568587200 ug
16698iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*592614200 ug
16704iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed*638655200 ug
16710iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed*654674200 ug
16712iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed*682701200 ug
16714iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*693713200 ug
16720iFluor® 750 goat anti-rabbit IgG (H+L) *Cross Adsorbed*753779200 ug
16721iFluor® 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*782811200 ug
16725trFluor™ Eu goat anti-rabbit IgG (H+L) *Cross Adsorbed*346617100 ug
16726trFluor™ Tb goat anti-rabbit IgG (H+L) *Cross Adsorbed*330544100 ug
16728HRP-labeled goat anti-mouse IgG (H+L)  1 mg
16729Biotinylated goat anti-mouse IgG (H+L)  1 mg
16730iFluor® 350 goat anti-mouse IgG (H+L)3454421 mg
16731iFluor® 405 goat anti-mouse IgG (H+L)4014201 mg
16735iFluor® 488 goat anti-mouse IgG (H+L)4915141 mg
16736iFluor® 514 goat anti-mouse IgG (H+L)5115271 mg
16737iFluor® 532 goat anti-mouse IgG (H+L)5365601 mg
16739iFluor® 555 goat anti-mouse IgG (H+L)5525671 mg
16741iFluor® 594 goat anti-mouse IgG (H+L)5926141 mg
16743iFluor® 633 goat anti-mouse IgG (H+L)6386551 mg
16744iFluor® 647 goat anti-mouse IgG (H+L)6546741 mg
16745iFluor® 680 goat anti-mouse IgG (H+L)6827011 mg
16746iFluor® 700 goat anti-mouse IgG (H+L)6937131 mg
16748iFluor® 750 goat anti-mouse IgG (H+L)7537791 mg
16750iFluor® 790 goat anti-mouse IgG (H+L)7828111 mg
16755trFluor™ Eu goat anti-mouse IgG (H+L)3466171 mg
16756trFluor™ Tb goat anti-mouse IgG (H+L)3305441 mg
16770iFluor® 350 goat anti-mouse IgG (H+L) *Cross Adsorbed*3454421 mg
16771iFluor® 405 goat anti-mouse IgG (H+L) *Cross Adsorbed*4014201 mg
16773iFluor® 488 goat anti-mouse IgG (H+L) *Cross Adsorbed*4915141 mg
16774iFluor® 514 goat anti-mouse IgG (H+L) *Cross Adsorbed*5115271 mg
16775iFluor® 532 goat anti-mouse IgG (H+L) *Cross Adsorbed*5365601 mg
16776iFluor® 555 goat anti-mouse IgG (H+L) *Cross Adsorbed*5525671 mg
16777iFluor® 568 goat anti-mouse IgG (H+L) *Cross Adsorbed*5685871 mg
16780iFluor® 594 goat anti-mouse IgG (H+L) *Cross Adsorbed*5926141 mg
16782iFluor® 633 goat anti-mouse IgG (H+L) *Cross Adsorbed*6386551 mg
16783iFluor® 647 goat anti-mouse IgG (H+L) *Cross Adsorbed*6546741 mg
16784iFluor® 680 goat anti-mouse IgG (H+L) *Cross Adsorbed*6827011 mg
16785iFluor® 700 goat anti-mouse IgG (H+L) *Cross Adsorbed*6937131 mg
16788iFluor® 750 goat anti-mouse IgG (H+L) *Cross Adsorbed*7537791 mg
16790iFluor® 790 goat anti-mouse IgG (H+L) *Cross Adsorbed*7828111 mg
16791trFluor™ Eu goat anti-mouse IgG (H+L) *Cross Adsorbed*3466171 mg
16792trFluor™ Tb goat anti-mouse IgG (H+L) *Cross Adsorbed*3305441 mg
16793HRP-labeled goat anti-rabbit IgG (H+L)  1 mg
16794Biotinylated goat anti-rabbit IgG (H+L)  1 mg
16795iFluor® 350 goat anti-rabbit IgG (H+L)3454421 mg
16796iFluor® 405 goat anti-rabbit IgG (H+L)4014201 mg
16800iFluor® 488 goat anti-rabbit IgG (H+L)4915141 mg
16801iFluor® 514 goat anti-rabbit IgG (H+L)5115271 mg
16802iFluor® 532 goat anti-rabbit IgG (H+L)5365601 mg
16803iFluor® 555 goat anti-rabbit IgG (H+L)5525671 mg
16804iFluor® 568 goat anti-rabbit IgG (H+L)5685871 mg
16806iFluor® 594 goat anti-rabbit IgG (H+L)5926141 mg
16808iFluor® 633 goat anti-rabbit IgG (H+L)6386551 mg
16809iFluor® 647 goat anti-rabbit IgG (H+L)6546741 mg
16810iFluor® 680 goat anti-rabbit IgG (H+L)6827011 mg
16811iFluor® 700 goat anti-rabbit IgG (H+L)6937131 mg
16813iFluor® 750 goat anti-rabbit IgG (H+L)7537791 mg
16815iFluor® 790 goat anti-rabbit IgG (H+L)7828111 mg
16820trFluor™ Eu goat anti-rabbit IgG (H+L)3466171 mg
16821trFluor™ Tb goat anti-rabbit IgG (H+L)3305441 mg
16825iFluor® 350 goat anti-rabbit IgG (H+L) *Cross Adsorbed*3454421 mg
16826iFluor® 405 goat anti-rabbit IgG (H+L) *Cross Adsorbed*4014201 mg
16828iFluor® 488 goat anti-rabbit IgG (H+L) *Cross Adsorbed*4915141 mg
16829iFluor® 514 goat anti-rabbit IgG (H+L) *Cross Adsorbed*5115271 mg
16830iFluor® 532 goat anti-rabbit IgG (H+L) *Cross Adsorbed*5365601 mg
16831iFluor® 555 goat anti-rabbit IgG (H+L) *Cross Adsorbed*5525671 mg
16832iFluor® 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*5685871 mg
16833iFluor® 594 goat anti-rabbit IgG (H+L) *Cross Adsorbed*5926141 mg
16835iFluor® 633 goat anti-rabbit IgG (H+L) *Cross Adsorbed*6386551 mg
16837iFluor® 647 goat anti-rabbit IgG (H+L) *Cross Adsorbed*6546741 mg
16838iFluor® 680 goat anti-rabbit IgG (H+L) *Cross Adsorbed*6827011 mg
16839iFluor® 700 goat anti-rabbit IgG (H+L) *Cross Adsorbed*6937131 mg
16842iFluor® 750 goat anti-rabbit IgG (H+L) *Cross Adsorbed*7537791 mg
16843iFluor® 790 goat anti-rabbit IgG (H+L) *Cross Adsorbed*7828111 mg
16847trFluor™ Eu goat anti-rabbit IgG (H+L) *Cross Adsorbed*3466171 mg
16848trFluor™ Tb goat anti-rabbit IgG (H+L) *Cross Adsorbed*3305441 mg
16852FITC goat anti-mouse IgG (H+L)4925151 mg
16853Texas Red® goat anti-mouse IgG (H+L)5826021 mg
16854Cy3® goat anti-mouse IgG (H+L)5555651 mg
16855Cy5® goat anti-mouse IgG (H+L)6496651 mg
16856Cy7® goat anti-mouse IgG (H+L)7497761 mg
16860FITC goat anti-mouse IgG (H+L) *Cross Adsorbed*4925151 mg
16861Texas Red® goat anti-mouse IgG (H+L) *Cross Adsorbed*5826021 mg
16862Cy3® goat anti-mouse IgG (H+L) *Cross Adsorbed*5555651 mg
16863Cy5® goat anti-mouse IgG (H+L) *Cross Adsorbed*6496651 mg
16864Cy7® goat anti-mouse IgG (H+L) *Cross Adsorbed*7497761 mg
16868FITC goat anti-rabbit IgG (H+L)4925151 mg
16869Texas Red® goat anti-rabbit IgG (H+L)5826021 mg
16870Cy3® goat anti-rabbit IgG (H+L)5555651 mg
16871Cy5® goat anti-rabbit IgG (H+L)6496651 mg
16872Cy7® goat anti-rabbit IgG (H+L)7497761 mg
16873APC-Cy7® tandem-labeled goat anti-rabbit IgG (H+L)651780100 ug
16876FITC goat anti-rabbit IgG (H+L) *Cross Adsorbed*4925151 mg
16877Texas Red® goat anti-rabbit IgG (H+L) *Cross Adsorbed*5826021 mg
16878Cy3® goat anti-rabbit IgG (H+L) *Cross Adsorbed*5555651 mg
16879Cy5® goat anti-rabbit IgG (H+L) *Cross Adsorbed*6496651 mg
16880Cy7® goat anti-rabbit IgG (H+L) *Cross Adsorbed*7497761 mg
50040iFluor® 350 Goat Anti-human IgG (H+L) Antibody  200 ug
50041iFluor® 350 Goat Anti-human IgG (H+L) Antibody  1 mg
50042iFluor® 350 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50043iFluor® 350 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50044iFluor® 405 Goat Anti-human IgG (H+L) Antibody  200 ug
50045iFluor® 405 Goat Anti-human IgG (H+L) Antibody  1 mg
50046iFluor® 405 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50047iFluor® 405 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50048iFluor® 430 Goat Anti-human IgG (H+L) Antibody  200 ug
50049iFluor® 430 Goat Anti-human IgG (H+L) Antibody  1 mg
50050iFluor® 430 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50051iFluor® 430 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50052iFluor® 450 Goat Anti-human IgG (H+L) Antibody  200 ug
50053iFluor® 450 Goat Anti-human IgG (H+L) Antibody  1 mg
50054iFluor® 450 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50055iFluor® 450 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50056iFluor® 488 Goat Anti-human IgG (H+L) Antibody  200 ug
50057iFluor® 488 Goat Anti-human IgG (H+L) Antibody  1 mg
50058iFluor® 488 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50059iFluor® 488 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50060iFluor® 514 Goat Anti-human IgG (H+L) Antibody  200 ug
50061iFluor® 514 Goat Anti-human IgG (H+L) Antibody  1 mg
50062iFluor® 514 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50063iFluor® 514 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50064iFluor® 532 Goat Anti-human IgG (H+L) Antibody  200 ug
50065iFluor® 532 Goat Anti-human IgG (H+L) Antibody  1 mg
50066iFluor® 532 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50067iFluor® 532 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50068iFluor® 546 Goat Anti-human IgG (H+L) Antibody  200 ug
50069iFluor® 546 Goat Anti-human IgG (H+L) Antibody  1 mg
50070iFluor® 546 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50071iFluor® 546 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50072iFluor® 555 Goat Anti-human IgG (H+L) Antibody  200 ug
50073iFluor® 555 Goat Anti-human IgG (H+L) Antibody  1 mg
50074iFluor® 555 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50075iFluor® 555 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50076iFluor® 560 Goat Anti-human IgG (H+L) Antibody  200 ug
50077iFluor® 560 Goat Anti-human IgG (H+L) Antibody  1 mg
50078iFluor® 560 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50079iFluor® 560 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50080iFluor® 568 Goat Anti-human IgG (H+L) Antibody  200 ug
50081iFluor® 568 Goat Anti-human IgG (H+L) Antibody  1 mg
50082iFluor® 568 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50083iFluor® 568 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50084iFluor® 594 Goat Anti-human IgG (H+L) Antibody  200 ug
50085iFluor® 594 Goat Anti-human IgG (H+L) Antibody  1 mg
50086iFluor® 594 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50087iFluor® 594 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50088iFluor® 610 Goat Anti-human IgG (H+L) Antibody  200 ug
50089iFluor® 610 Goat Anti-human IgG (H+L) Antibody  1 mg
50090iFluor® 610 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50091iFluor® 610 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50092iFluor® 633 Goat Anti-human IgG (H+L) Antibody  200 ug
50093iFluor® 633 Goat Anti-human IgG (H+L) Antibody  1 mg
50094iFluor® 633 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50095iFluor® 633 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50096iFluor® 647 Goat Anti-human IgG (H+L) Antibody  200 ug
50097iFluor® 647 Goat Anti-human IgG (H+L) Antibody  1 mg
50098iFluor® 647 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50099iFluor® 647 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50100iFluor® 660 Goat Anti-human IgG (H+L) Antibody  200 ug
50101iFluor® 660 Goat Anti-human IgG (H+L) Antibody  1 mg
50102iFluor® 660 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50103iFluor® 660 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50104iFluor® 670 Goat Anti-human IgG (H+L) Antibody  200 ug
50105iFluor® 670 Goat Anti-human IgG (H+L) Antibody  1 mg
50106iFluor® 670 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50107iFluor® 670 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50108iFluor® 680 Goat Anti-human IgG (H+L) Antibody  200 ug
50109iFluor® 680 Goat Anti-human IgG (H+L) Antibody  1 mg
50110iFluor® 680 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50111iFluor® 680 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50112iFluor® 700 Goat Anti-human IgG (H+L) Antibody  200 ug
50113iFluor® 700 Goat Anti-human IgG (H+L) Antibody  1 mg
50114iFluor® 700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50115iFluor® 700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50116iFluor® 710 Goat Anti-human IgG (H+L) Antibody  200 ug
50117iFluor® 710 Goat Anti-human IgG (H+L) Antibody  1 mg
50118iFluor® 710 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50119iFluor® 710 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50120iFluor® 750 Goat Anti-human IgG (H+L) Antibody  200 ug
50121iFluor® 750 Goat Anti-human IgG (H+L) Antibody  1 mg
50122iFluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50123iFluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50124iFluor® 790 Goat Anti-human IgG (H+L) Antibody  200 ug
50125iFluor® 790 Goat Anti-human IgG (H+L) Antibody  1 mg
50126iFluor® 790 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50127iFluor® 790 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50128iFluor® 800 Goat Anti-human IgG (H+L) Antibody  200 ug
50129iFluor® 800 Goat Anti-human IgG (H+L) Antibody  1 mg
50130iFluor® 800 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50131iFluor® 800 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50132iFluor® 810 Goat Anti-human IgG (H+L) Antibody  200 ug
50133iFluor® 810 Goat Anti-human IgG (H+L) Antibody  1 mg
50134iFluor® 810 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50135iFluor® 810 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50136iFluor® 820 Goat Anti-human IgG (H+L) Antibody  200 ug
50137iFluor® 820 Goat Anti-human IgG (H+L) Antibody  1 mg
50138iFluor® 820 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50139iFluor® 820 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50140iFluor® 840 Goat Anti-human IgG (H+L) Antibody  200 ug
50141iFluor® 840 Goat Anti-human IgG (H+L) Antibody  1 mg
50142iFluor® 840 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50143iFluor® 840 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50144iFluor® 860 Goat Anti-human IgG (H+L) Antibody  200 ug
50145iFluor® 860 Goat Anti-human IgG (H+L) Antibody  1 mg
50146iFluor® 860 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50147iFluor® 860 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50148iFluor® A7 Goat Anti-human IgG (H+L) Antibody  200 ug
50149iFluor® A7 Goat Anti-human IgG (H+L) Antibody  1 mg
50150iFluor® A7 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50151iFluor® A7 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50152XFD350 Goat Anti-human IgG (H+L) Antibody *XFD350 Same Structure to Alexa Fluor™ 350*  200 ug
50153XFD350 Goat Anti-human IgG (H+L) Antibody *XFD350 Same Structure to Alexa Fluor™ 350*  1 mg
50154XFD350 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*  200 ug
50155XFD350 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD350 Same Structure to Alexa Fluor™ 350*  1 mg
50156XFD488 Goat Anti-human IgG (H+L) Antibody *XFD488 Same Structure to Alexa Fluor™ 488*  200 ug
50157XFD488 Goat Anti-human IgG (H+L) Antibody *XFD488 Same Structure to Alexa Fluor™ 488*  1 mg
50158XFD488 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*  200 ug
50159XFD488 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD488 Same Structure to Alexa Fluor™ 488*  1 mg
50160XFD555 Goat Anti-human IgG (H+L) Antibody *XFD555 Same Structure to Alexa Fluor™ 555*  200 ug
50161XFD555 Goat Anti-human IgG (H+L) Antibody *XFD555 Same Structure to Alexa Fluor™ 555*  1 mg
50162XFD555 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD555 Same Structure to Alexa Fluor™ 555*  200 ug
50163XFD555 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD555 Same Structure to Alexa Fluor™ 555*  1 mg
50164XFD594 Goat Anti-human IgG (H+L) Antibody *XFD594 Same Structure to Alexa Fluor™ 594*  200 ug
50165XFD594 Goat Anti-human IgG (H+L) Antibody *XFD594 Same Structure to Alexa Fluor™ 594*  1 mg
50166XFD594 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*  200 ug
50167XFD594 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD594 Same Structure to Alexa Fluor™ 594*  1 mg
50168XFD647 Goat Anti-human IgG (H+L) Antibody *XFD647 Same Structure to Alexa Fluor™ 647*  200 ug
50169XFD647 Goat Anti-human IgG (H+L) Antibody *XFD647 Same Structure to Alexa Fluor™ 647*  1 mg
50170XFD647 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD647 Same Structure to Alexa Fluor™ 647*  200 ug
50171XFD647 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD647 Same Structure to Alexa Fluor™ 647*  1 mg
50172XFD680 Goat Anti-human IgG (H+L) Antibody *XFD680 Same Structure to Alexa Fluor™ 680*  200 ug
50173XFD680 Goat Anti-human IgG (H+L) Antibody *XFD680 Same Structure to Alexa Fluor™ 680*  1 mg
50174XFD680 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD680 Same Structure to Alexa Fluor™ 680*  200 ug
50175XFD680 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD680 Same Structure to Alexa Fluor™ 680*  1 mg
50176XFD700 Goat Anti-human IgG (H+L) Antibody *XFD700 Same Structure to Alexa Fluor™ 700*  200 ug
50177XFD700 Goat Anti-human IgG (H+L) Antibody *XFD700 Same Structure to Alexa Fluor™ 700*  1 mg
50178XFD700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD700 Same Structure to Alexa Fluor™ 700*  200 ug
50179XFD700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD700 Same Structure to Alexa Fluor™ 700*  1 mg
50180XFD750 Goat Anti-human IgG (H+L) Antibody *XFD750 Same Structure to Alexa Fluor™ 750*  200 ug
50181XFD750 Goat Anti-human IgG (H+L) Antibody *XFD750 Same Structure to Alexa Fluor™ 750*  1 mg
50182XFD750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD750 Same Structure to Alexa Fluor™ 750*  200 ug
50183XFD750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD750 Same Structure to Alexa Fluor™ 750*  1 mg
50184APC Goat Anti-human IgG (H+L) Antibody  200 ug
50185APC Goat Anti-human IgG (H+L) Antibody  1 mg
50186APC Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50187APC Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50188APC/Cy7 Goat Anti-human IgG (H+L) Antibody  200 ug
50189APC/Cy7 Goat Anti-human IgG (H+L) Antibody  1 mg
50190APC/Cy7 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50191APC/Cy7 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50192APC/XFD750 Goat Anti-human IgG (H+L) Antibody *XFD750 Same Structure to Alexa Fluor™ 750*  200 ug
50193APC/XFD750 Goat Anti-human IgG (H+L) Antibody *XFD750 Same Structure to Alexa Fluor™ 750*  1 mg
50194APC/XFD750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD750 Same Structure to Alexa Fluor™ 750*  200 ug
50195APC/XFD750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed, XFD750 Same Structure to Alexa Fluor™ 750*  1 mg
50196APC/iFluor® 700 Goat Anti-human IgG (H+L) Antibody  200 ug
50197APC/iFluor® 700 Goat Anti-human IgG (H+L) Antibody  1 mg
50198APC/iFluor® 700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50199APC/iFluor® 700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50200APC/iFluor® 750 Goat Anti-human IgG (H+L) Antibody  200 ug
50201APC/iFluor® 750 Goat Anti-human IgG (H+L) Antibody  1 mg
50202APC/iFluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50203APC/iFluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50204FITC Goat Anti-human IgG (H+L) Antibody  200 ug
50205FITC Goat Anti-human IgG (H+L) Antibody  1 mg
50206FITC Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50207FITC Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50208TRITC Goat Anti-human IgG (H+L) Antibody  200 ug
50209TRITC Goat Anti-human IgG (H+L) Antibody  1 mg
50210TRITC Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50211TRITC Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50212PacBlue Goat Anti-human IgG (H+L) Antibody  200 ug
50213PacBlue Goat Anti-human IgG (H+L) Antibody  1 mg
50214PacBlue Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50215PacBlue Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50216PacOrange Goat Anti-human IgG (H+L) Antibody  200 ug
50217PacOrange Goat Anti-human IgG (H+L) Antibody  1 mg
50218PacOrange Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50219PacOrange Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50220PE Goat Anti-human IgG (H+L) Antibody  200 ug
50221PE Goat Anti-human IgG (H+L) Antibody  1 mg
50222PE Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50223PE Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50224PE/Cy5 Goat Anti-human IgG (H+L) Antibody  200 ug
50225PE/Cy5 Goat Anti-human IgG (H+L) Antibody  1 mg
50226PE/Cy5 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50227PE/Cy5 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50228PE/Cy7 Goat Anti-human IgG (H+L) Antibody  200 ug
50229PE/Cy7 Goat Anti-human IgG (H+L) Antibody  1 mg
50230PE/Cy7 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50231PE/Cy7 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50232PE/AF 610 Goat Anti-human IgG (H+L) Antibody  200 ug
50233PE/AF 610 Goat Anti-human IgG (H+L) Antibody  1 mg
50234PE/AF 610 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50235PE/AF 610 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50236PE/AF 700 Goat Anti-human IgG (H+L) Antibody  200 ug
50237PE/AF 700 Goat Anti-human IgG (H+L) Antibody  1 mg
50238PE/AF 700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50239PE/AF 700 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50240PE/iFluor® 647 Goat Anti-human IgG (H+L) Antibody  200 ug
50241PE/iFluor® 647 Goat Anti-human IgG (H+L) Antibody  1 mg
50242PE/iFluor® 647 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50243PE/iFluor® 647 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50244PE/iFluor® 750 Goat Anti-human IgG (H+L) Antibody  200 ug
50245PE/iFluor® 750 Goat Anti-human IgG (H+L) Antibody  1 mg
50246PE/iFluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50247PE/iFluor® 750 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50248PE/Texas Red® Goat Anti-human IgG (H+L) Antibody  200 ug
50249PE/Texas Red® Goat Anti-human IgG (H+L) Antibody  1 mg
50250PE/Texas Red® Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50251PE/Texas Red® Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50252PerCP Goat Anti-human IgG (H+L) Antibody  200 ug
50253PerCP Goat Anti-human IgG (H+L) Antibody  1 mg
50254PerCP Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50255PerCP Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50256PerCP/Cy5.5 Goat Anti-human IgG (H+L) Antibody  200 ug
50257PerCP/Cy5.5 Goat Anti-human IgG (H+L) Antibody  1 mg
50258PerCP/Cy5.5 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50259PerCP/Cy5.5 Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50260HRP Goat Anti-human IgG (H+L) Antibody  200 ug
50261HRP Goat Anti-human IgG (H+L) Antibody  1 mg
50262HRP Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50263HRP Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50264Biotin Goat Anti-human IgG (H+L) Antibody  200 ug
50265Biotin Goat Anti-human IgG (H+L) Antibody  1 mg
50266Biotin Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  200 ug
50267Biotin Goat Anti-human IgG (H+L) Antibody *Cross Adsorbed*  1 mg
50269Purified Goat Anti-human IgG (H+L) Antibody  1 mg
50273Purified Goat Anti-human IgG Fc (H+L) Antibody  1 mg
50301Goat anti human IgG (Fc) serum  1 mL
50302Goat anti human IgG (Fc) serum  10 mL
55010Goat anti-mouse IgG (H&L) Agarose  10 mg
55015Goat anti-rabbit IgG (H&L) Agarose  10 mg