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iFluor™ 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed*

The excitation and emission spectra of iFluor<sup>TM</sup> 568. 
The excitation and emission spectra of iFluor<sup>TM</sup> 568. 
Ordering information
Price ()
Catalog Number16692
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight150000
Spectral properties
Correction Factor (260 nm)0.34
Correction Factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)1000001
Excitation (nm)568
Emission (nm)587
Quantum yield0.571
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
150000
Correction Factor (260 nm)
0.34
Correction Factor (280 nm)
0.15
Extinction coefficient (cm -1 M -1)
1000001
Excitation (nm)
568
Emission (nm)
587
Quantum yield
0.571
iFluor™ 568 is a bright red fluorescent dye. iFluor™ 568-labeled anti-IgG conjugates exhibit bright fluorescence signal and good photostability. Used for stable signal generation in imaging and flow cytometry, the fluorescence intensity of iFluor™ 568 conjugates is pH-insensitive from pH 4 to pH 11. The iFluor™ 568-labeled antibody conjugates can be well excited with Krypton ion laser (~568 nm). iFluor™ 568 family has the spectral properties essentially identical to those of Alexa Fluor® 568. Under the same conditions we tested, iFluor™ 568 antibody conjugates are brighter and more photostable than the corresponding Alexa Fluo® 568. These spectral and labeling characteristics make the iFluor™ 568 dye family a superior alternative to Alexa Fluor® 568. In addition, iFluor™ 568 secondary antibody conjugates give higher signal/background ratios than the corresponding Alexa Fluor® 568-labeled conjugates.

Calculators


Common stock solution preparation

Table 1. Volume of appropriate solvent needed to reconstitute specific mass of iFluor™ 568 goat anti-rabbit IgG (H+L) *Cross Adsorbed* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM666.667 nL3.333 µL6.667 µL33.333 µL66.667 µL
5 mM133.333 nL666.667 nL1.333 µL6.667 µL13.333 µL
10 mM66.667 nL333.333 nL666.667 nL3.333 µL6.667 µL

Molarity calculator

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Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.34
Correction Factor (280 nm)0.15
Extinction coefficient (cm -1 M -1)1000001
Excitation (nm)568
Emission (nm)587
Quantum yield0.571

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor™ 568 goat anti-mouse IgG (H+L)56858710000010.5710.340.15
iFluor™ 568 goat anti-rabbit IgG (H+L)56858710000010.5710.340.15
Biotinylated goat anti-rabbit IgG (H+L)------
Cy3® goat anti-rabbit IgG (H+L)55556915000010.1510.070.073
Cy5® goat anti-rabbit IgG (H+L)65167025000010.271, 0.420.020.03
Cy7® goat anti-rabbit IgG (H+L)7567792500000.30.050.036
FITC goat anti-rabbit IgG (H+L)491516730000.92-0.254
HRP-labeled goat anti-rabbit IgG (H+L)------
Texas Red® goat anti-rabbit IgG (H+L)58660311600010.931, 0.3520.230.36

Citations


View all 4 citations: Citation Explorer
Overexpression of CXCR2 predicts poor prognosis in patients with colorectal cancer.
Authors: Zhao, Jingkun and Ou, Baochi and Feng, Hao and Wang, Puxiongzhi and Yin, Shuai and Zhu, Congcong and Wang, Shenjie and Chen, Chun and Zheng, Minhua and Zong, Yaping and others, undefined
Journal: Oncotarget (2017)
Cadherin-12 enhances proliferation in colorectal cancer cells and increases progression by promoting EMT
Authors: Ma, Junjun and Zhao, Jingkun and Lu, Jun and Wang, Puxiongzhi and Feng, Hao and Zong, Yaping and Ou, Baochi and Zheng, Minhua and Lu, Aiguo
Journal: Tumor Biology (2016): 1--12
Transplantation of RADA16-BDNF peptide scaffold with human umbilical cord mesenchymal stem cells forced with CXCR4 and activated astrocytes for repair of traumatic brain injury
Authors: Shi, W and Huang, CJ and Xu, XD and Jin, GH and Huang, RQ and Huang, JF and Chen, YN and Ju, SQ and Wang, Y and Shi, YW and others, undefined
Journal: Acta Biomaterialia (2016): 247--261
The migration and differentiation of hUC-MSCsCXCR4/GFP encapsulated in BDNF/chitosan scaffolds for brain tissue engineering
Authors: Huang, Chuanjun and Zhao, Longxiang and Gu, Jun and Nie, Dekang and Chen, Yinan and Zuo, Hao and Huan, Wei and Shi, Jinlong and Chen, Jian and Shi, Wei
Journal: Biomedical Materials (2016): 035004

References


View all 191 references: Citation Explorer
Antiprothrombin antibodies in a patient with secondary antiphospholipid syndrome and bleeding
Authors: Gonzalez Leon R, Garcia Hern and ez FJ, Castillo Palma MJ, Sanchez Roman J.
Journal: Med Clin (Barc) (2011): 668
Assessment of EGFR/HER2 dimerization by FRET-FLIM utilizing Alexa-conjugated secondary antibodies in relation to targeted therapies in cancers
Authors: Waterhouse BR, Gijsen M, Barber PR, Tullis ID, Vojnovic B, Kong A.
Journal: Oncotarget (2011): 728
Falsely elevated tacrolimus levels caused by immunoassay interference secondary to beta-galactosidase antibodies in an infected liver transplant recipient
Authors: Knorr JP, Grewal KS, Balasubramanian M, Young N, Zaki R, Khanmoradi K, Araya V, Ortiz J.
Journal: Pharmacotherapy (2010): 954
Prevalence of anti-Epstein-Barr virus antibodies in children and adolescents with secondary immunodeficiency
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Journal: Epidemiol Mikrobiol Imunol (2010): 133
Analysis of the effectiveness of reused primary and secondary antibodies in Western blotting analysis
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Journal: Anal Biochem (2010): 124
Nanosilver-doped DNA polyion complex membrane for electrochemical immunoassay of carcinoembryonic antigen using nanogold-labeled secondary antibodies
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Journal: Anal Chim Acta (2010): 126
Three siblings with self-resolving congenital hyperthyrotropinaemia secondary to thyrotropin receptor blocking antibodies
Authors: Azzopardi P, Forrester M, Ehtisham S.
Journal: J Paediatr Child Health (2010): 439
Double-blotting: a solution to the problem of nonspecific binding of secondary antibodies in immunoblotting procedures
Authors: Lasne F., undefined
Journal: Methods Mol Biol (2009): 213
Parkinsonism/dystonia syndrome secondary to multiple sclerosis with anti-basal-ganglia antibodies
Authors: Delgado S, Baez S, Singer C, Sengun C, Sheremata WA, Papapetropoulos S.
Journal: Mov Disord (2009): 309
Neutralizing antibodies and secondary therapy failure after treatment with botulinum toxin type A: much ado about nothing
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Journal: Clin Neuropharmacol (2009): 213