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Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*

Chemical structure for Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*
Chemical structure for Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*
Ordering information
Price ()
Catalog Number11000
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight257.24
SolventDMSO
Spectral properties
Excitation (nm)571
Emission (nm)584
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
119171-73-2
Molecular weight
257.24
Excitation (nm)
571
Emission (nm)
584
Our Amplite® ADHP is chemically same to Amplex™ Red (Amplex™ Red is the trademark of Invitrogen). It is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM388.742 µL1.944 mL3.887 mL19.437 mL38.874 mL
5 mM77.748 µL388.742 µL777.484 µL3.887 mL7.775 mL
10 mM38.874 µL194.371 µL388.742 µL1.944 mL3.887 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)571
Emission (nm)584

Product family


NameExcitation (nm)Emission (nm)
Amplite® Blue--
Amplite® IR648668
Amplite® Red571584

Citations


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Journal: The American Journal of Human Genetics (2020): 527--538
C3 opsonization of anthrax bacterium and peptidoglycan supports recognition and activation of neutrophils
Authors: Popescu, Narcis I and Keshari, Ravi S and Cochran, Jackie and Coggeshall, K Mark and Lupu, Florea
Journal: Microorganisms (2020): 1039
Multimodal Molecular Imaging Demonstrates Myeloperoxidase Regulation of Matrix Metalloproteinase Activity in Neuroinflammation
Authors: Zhang, Yinian and Dong, Huateng and Seeburg, Daniel P and Wojtkiewicz, Gregory R and Waterman, Peter and Pulli, Benjamin and Forghani, Reza and Ali, Muhammad and Iwamoto, Yoshiko and Swirski, Filip K and others, undefined
Journal: Molecular neurobiology (2018): 1--9
Spinal cord inflammation: molecular imaging after thoracic aortic ischemia reperfusion injury
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: Radiology (2017): 202--211
Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical Chemistry (2017)
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Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical chemistry (2017): 7727--7733
Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: Radiology (2016): 152222
Impact of isolated burns on major organs: a large animal model characterized
Authors: Burmeister, David M and McIntyre, Matthew K and Baker, Bryan A and Rizzo, Julie A and Brown, Ammon and Natesan, Shanmugasundaram and Chung, Kevin K and Christy, Robert J
Journal: Shock (2016): 137--147

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