Amplite® Red

Our Amplite® Red is a sensitive fluorogenic peroxidase substrate that generates a highly red fluorescent product that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of Amplite® Red is minimal. Amplite® Red is one of the most sensitive and stable fluorogenic probes for detecting HRP and H2O2. Amplite® Red has been widely used to detect HRP in many immunoassays. On the other hand, Amplite® Red can also be used to detect trace amount of H2O2. The Amplite® Red-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, Amplite® Red can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.

HRP dose response was measured with Amplite® Fluorimetric Peroxidase Assay Kit in a black plate using a Gemini fluorescence microplate reader (Molecular Devices).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11011	1000 Assays	Price

Citations

View all 11 citations: Citation Explorer

Enzymatic Methods

Authors:

Khelfi, A

Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase

Authors:

Milton, Amber

Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA

Authors:

Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze

Journal:

Analytical Chemistry (2017)

Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury

Authors:

Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T

Journal:

Radiology (2016): 152222

Myeloperoxidase--Hepatocyte--Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis

Authors:

Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W

Journal:

Antioxidants & redox signaling (2015): 1255--1269

References

View all 61 references: Citation Explorer

Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor

Authors:

Fruk L, Muller J, Niemeyer CM.

Journal:

Chemistry (2006): 7448

Real-time assessment of spatial and temporal coupled catalysis within polyelectrolyte microcapsules containing coimmobilized glucose oxidase and peroxidase

Authors:

Stein EW, Volodkin DV, McShane MJ, Sukhorukov GB.

Journal:

Biomacromolecules (2006): 710

O2 Delivery and Redox State are Determinants of Compartment-specific Reactive Oxygen Species in Myocardial Reperfusion

Authors:

Stoner JD, Clanton TL, Aune SE, Angelos MG.

Journal:

Am J Physiol Heart Circ Physiol. (2006)

Fibrillar beta-amyloid peptide Abeta1-40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: a cell culture study

Authors:

Jekabsone A, M and er PK, Tickler A, Sharpe M, Brown GC.

Journal:

J Neuroinflammation (2006): 24

Biochemical activity of reactive oxygen species scavengers do not predict retinal ganglion cell survival

Authors:

Schlieve CR, Lieven CJ, Levin LA.

Journal:

Invest Ophthalmol Vis Sci (2006): 3878

Physical properties

Molecular weight	N/A
Solvent	DMSO

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure; Desiccated
UNSPSC	12171501

Instrument settings

Fluorescence microplate reader
Excitation	540 nm
Emission	590 nm
Cutoff	550 nm
Recommended plate	Solid black

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 23, 2025