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Amplite® IR
Our Amplite® IR is a fluorogenic peroxidase substrate that generates near infrared fluorescence upon reaction with peroxidase and H2O2. It can be used to detect both H2O2 and peroxidase. Amplite® IR generates a substance that has maximum absorption of 647 nm with maximum emission at 670 nm. This near infrared absorption and fluorescence minimize the assay background that is often caused by the autoabsorption and/or autofluorescence of biological samples that rarely absorb light beyond 600 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of Amplite® IR is minimal. Compared to Amplex Red™, Amplite® IR generates the fluorescence that is pH-independent from pH 4 to 10. In addition, it has excellent water solubility. It is a superior alternative to Amplex Red™ for the detections that require low pH where Amplex Red™ has significantly reduced fluorescence. We have used Amplite® IR to detect HRP in quite a few immunoassays. Amplite® IR can also be used to detect trace amount of H2O2. Because H2O2 is produced in many enzymatic redox reactions, Amplite® IR can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids etc.
H2O2 dose response was measured in a solid black 96-well plate with Amplite® Fluorimetric Hydrogen Peroxide Assay Kit.
H2O2 dose response was measured in a solid black 96-well plate with Amplite® Fluorimetric Hydrogen Peroxide Assay Kit.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
110091 mg
Price
 
Physical properties
Molecular weight~400
SolventDMSO
Spectral properties
Excitation (nm)648
Emission (nm)668
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure; Desiccated
UNSPSC12171501
Instrument settings
Fluorescence microplate reader
Excitation640 nm
Emission680 nm
Cutoff650 nm
Recommended plateSolid black
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Page updated on October 8, 2025