Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*

Amplite® ADHP

10-Acetyl-3,7-dihydroxyphenoxazine; CAS#: 119171-73-2

Our Amplite® ADHP is chemically same to Amplex™ Red (Amplex™ Red is the trademark of Invitrogen). It is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.

Protocol

SDS

Catalog	Size	Price	Quantity
11000	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	388.742 µL	1.944 mL	3.887 mL	19.437 mL	38.874 mL
5 mM	77.748 µL	388.742 µL	777.484 µL	3.887 mL	7.775 mL
10 mM	38.874 µL	194.371 µL	388.742 µL	1.944 mL	3.887 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	257.24 g/mol	=	mL	x	mM	=	mmol

Citations

View all 20 citations: Citation Explorer

Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy

Authors:

Ali, Muhammad and Fulci, Giulia and Grigalavicius, Mantas and Pulli, Benjamin and Li, Anning and Wojtkiewicz, Gregory R and Wang, Cuihua and Hsieh, Kevin Li-Chun and Linnoila, Jenny J and Theodossiou, Theodossis A and others,

Journal:

Neoplasia (2022): 100779

C3 opsonization of anthrax bacterium and peptidoglycan supports recognition and activation of neutrophils

Authors:

Popescu, Narcis I and Keshari, Ravi S and Cochran, Jackie and Coggeshall, K Mark and Lupu, Florea

Journal:

Microorganisms (2020): 1039

Myeloperoxidase modulates inflammation in generalized pustular psoriasis and additional rare pustular skin diseases

Authors:

Haskamp, Stefan and Bruns, Heiko and Hahn, Madelaine and Hoffmann, Markus and Gregor, Anne and L{\"o}hr, Sabine and Hahn, Jonas and Schauer, Christine and Ringer, Mark and Flamann, Cindy and others,

Journal:

The American Journal of Human Genetics (2020): 527--538

Multimodal Molecular Imaging Demonstrates Myeloperoxidase Regulation of Matrix Metalloproteinase Activity in Neuroinflammation

Authors:

Zhang, Yinian and Dong, Huateng and Seeburg, Daniel P and Wojtkiewicz, Gregory R and Waterman, Peter and Pulli, Benjamin and Forghani, Reza and Ali, Muhammad and Iwamoto, Yoshiko and Swirski, Filip K and others, undefined

Journal:

Molecular neurobiology (2018): 1--9

Patterned photonic nitrocellulose for pseudopaper ELISA

Authors:

Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze

Journal:

Analytical chemistry (2017): 7727--7733

References

View all 61 references: Citation Explorer

Volatile fatty acid-sensing system involving coenzyme-A transferase

Authors:

Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K.

Journal:

Biotechnol Prog (2006): 334

Real-time assessment of spatial and temporal coupled catalysis within polyelectrolyte microcapsules containing coimmobilized glucose oxidase and peroxidase

Authors:

Stein EW, Volodkin DV, McShane MJ, Sukhorukov GB.

Journal:

Biomacromolecules (2006): 710

O2 Delivery and Redox State are Determinants of Compartment-specific Reactive Oxygen Species in Myocardial Reperfusion

Authors:

Stoner JD, Clanton TL, Aune SE, Angelos MG.

Journal:

Am J Physiol Heart Circ Physiol. (2006)

Fibrillar beta-amyloid peptide Abeta1-40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: a cell culture study

Authors:

Jekabsone A, M and er PK, Tickler A, Sharpe M, Brown GC.

Journal:

J Neuroinflammation (2006): 24

Biochemical activity of reactive oxygen species scavengers do not predict retinal ganglion cell survival

Authors:

Schlieve CR, Lieven CJ, Levin LA.

Journal:

Invest Ophthalmol Vis Sci (2006): 3878

Physical properties

Molecular weight	257.24
Solvent	DMSO

Spectral properties

Excitation (nm)	571
Emission (nm)	584

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	119171-73-2

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis
Brochure: Buccutite™ Crosslinking Technology
Brochure: Enzyme Probes & Assay Kits
Brochure: Reactive Oxygen Species (ROS) Detection

Telephone
Fax
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Page updated on October 30, 2025