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Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*
Our Amplite® ADHP is chemically same to Amplex™ Red (Amplex™ Red is the trademark of Invitrogen). It is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 388.742 µL | 1.944 mL | 3.887 mL | 19.437 mL | 38.874 mL |
| 5 mM | 77.748 µL | 388.742 µL | 777.484 µL | 3.887 mL | 7.775 mL |
| 10 mM | 38.874 µL | 194.371 µL | 388.742 µL | 1.944 mL | 3.887 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) |
| Amplite® IR | 648 | 668 |
| Amplite® Red | 571 | 584 |
Citations
View all 20 citations: Citation Explorer
Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy
Authors: Ali, Muhammad and Fulci, Giulia and Grigalavicius, Mantas and Pulli, Benjamin and Li, Anning and Wojtkiewicz, Gregory R and Wang, Cuihua and Hsieh, Kevin Li-Chun and Linnoila, Jenny J and Theodossiou, Theodossis A and others,
Journal: Neoplasia (2022): 100779
Authors: Ali, Muhammad and Fulci, Giulia and Grigalavicius, Mantas and Pulli, Benjamin and Li, Anning and Wojtkiewicz, Gregory R and Wang, Cuihua and Hsieh, Kevin Li-Chun and Linnoila, Jenny J and Theodossiou, Theodossis A and others,
Journal: Neoplasia (2022): 100779
C3 opsonization of anthrax bacterium and peptidoglycan supports recognition and activation of neutrophils
Authors: Popescu, Narcis I and Keshari, Ravi S and Cochran, Jackie and Coggeshall, K Mark and Lupu, Florea
Journal: Microorganisms (2020): 1039
Authors: Popescu, Narcis I and Keshari, Ravi S and Cochran, Jackie and Coggeshall, K Mark and Lupu, Florea
Journal: Microorganisms (2020): 1039
Myeloperoxidase modulates inflammation in generalized pustular psoriasis and additional rare pustular skin diseases
Authors: Haskamp, Stefan and Bruns, Heiko and Hahn, Madelaine and Hoffmann, Markus and Gregor, Anne and L{\"o}hr, Sabine and Hahn, Jonas and Schauer, Christine and Ringer, Mark and Flamann, Cindy and others,
Journal: The American Journal of Human Genetics (2020): 527--538
Authors: Haskamp, Stefan and Bruns, Heiko and Hahn, Madelaine and Hoffmann, Markus and Gregor, Anne and L{\"o}hr, Sabine and Hahn, Jonas and Schauer, Christine and Ringer, Mark and Flamann, Cindy and others,
Journal: The American Journal of Human Genetics (2020): 527--538
Multimodal Molecular Imaging Demonstrates Myeloperoxidase Regulation of Matrix Metalloproteinase Activity in Neuroinflammation
Authors: Zhang, Yinian and Dong, Huateng and Seeburg, Daniel P and Wojtkiewicz, Gregory R and Waterman, Peter and Pulli, Benjamin and Forghani, Reza and Ali, Muhammad and Iwamoto, Yoshiko and Swirski, Filip K and others, undefined
Journal: Molecular neurobiology (2018): 1--9
Authors: Zhang, Yinian and Dong, Huateng and Seeburg, Daniel P and Wojtkiewicz, Gregory R and Waterman, Peter and Pulli, Benjamin and Forghani, Reza and Ali, Muhammad and Iwamoto, Yoshiko and Swirski, Filip K and others, undefined
Journal: Molecular neurobiology (2018): 1--9
Patterned photonic nitrocellulose for pseudopaper ELISA
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical chemistry (2017): 7727--7733
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical chemistry (2017): 7727--7733
References
View all 61 references: Citation Explorer
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Authors: Rajashekhara E, Hosoda A, Sode K, Ikenaga H, Watanabe K.
Journal: Biotechnol Prog (2006): 334
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Fibrillar beta-amyloid peptide Abeta1-40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: a cell culture study
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Authors: Jekabsone A, M and er PK, Tickler A, Sharpe M, Brown GC.
Journal: J Neuroinflammation (2006): 24
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Authors: Schlieve CR, Lieven CJ, Levin LA.
Journal: Invest Ophthalmol Vis Sci (2006): 3878
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