Amplite™ ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*

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Chemical structure for Amplite™ ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*
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Unit Size: Cat No: Price (USD): Qty:
25 mg 11000 $195


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Overview

Ex/Em (nm)570/583
MW257.24
CAS #119171-73-2
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
Category Oxidases,
Horseradish Peroxidase (HRP) and Poly-HRP,
Reactive Oxygen Species (ROS)
Our Amplite™ ADHP is chemically same to Amplex® Red (Amplex® Red is the trademark of Invitrogen). It is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Amplite™ ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



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Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
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References

Multimodal Molecular Imaging Demonstrates Myeloperoxidase Regulation of Matrix Metalloproteinase Activity in Neuroinflammation
Authors: Yinian Zhang, Huateng Dong, Daniel P Seeburg, Gregory R Wojtkiewicz, Peter Waterman, Benjamin Pulli, Reza Forghani, Muhammad Ali, Yoshiko Iwamoto, Filip K Swirski
Journal: Molecular neurobiology (2018): 1--9

Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase
Authors: Amber Milton
Journal: (2017)

Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA
Authors: Junjie Chi, Bingbing Gao, Mi Sun, Fengling Zhang, Enben Su, Hong Liu, Zhongze Gu
Journal: Analytical Chemistry (2017)

Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury
Authors: Hassan Albadawi, John W Chen, Rahmi Oklu, Yue Wu, Gregory Wojtkiewicz, Benjamin Pulli, John D Milner, Richard P Cambria, Michael T Watkins
Journal: Radiology (2016): 152222

Myeloperoxidase Nuclear Imaging for Epileptogenesis
Authors: Yinian Zhang, Daniel P Seeburg, Benjamin Pulli, Gregory R Wojtkiewicz, Lionel Bure, Wendy Atkinson, Stefan Schob, Yoshiko Iwamoto, Muhammad Ali, Wei Zhang
Journal: Radiology (2015): 822--830

Myeloperoxidase--Hepatocyte--Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
Authors: Benjamin Pulli, Muhammad Ali, Yoshiko Iwamoto, Matthias WG Zeller, Stefan Schob, Jenny J Linnoila, John W Chen
Journal: Antioxidants & redox signaling (2015): 1255--1269

Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs
Authors: Jiansheng Huang, Forrest Smith, Peter Panizzi
Journal: Archives of biochemistry and biophysics (2014): 74--85

Raising the shields: PCR in the presence of metallic surfaces protected by tailor-made coatings
Authors: Frank D Scherag, Thomas Brandstetter, Jürgen Rühe
Journal: Colloids and Surfaces B: Biointerfaces (2014): 576--582

Measuring myeloperoxidase activity in biological samples
Authors: Benjamin Pulli, Muhammad Ali, Reza Forghani, Stefan Schob, Kevin LC Hsieh, Gregory Wojtkiewicz, Jenny J Linnoila, John W Chen
Journal: PLoS One (2013): e67976

Micro-volume wall-less immunoassays using patterned planar plates
Authors: Katherine R Kozak, Jianyong Wang, Melvin Lye, Rashi Takkar, Namyong Kim, Hyunjae Lee, Noo Li Jeon, Kedan Lin, Crystal Zhang, Wai Lee T Wong
Journal: Lab on a Chip (2013): 1342--1350


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