Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*
Ordering information
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Availability | In stock |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 257.24 |
Solvent | DMSO |
Spectral properties
Excitation (nm) | 571 |
Emission (nm) | 584 |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Amplite® Reagents and Assay Kits, Protein to Protein Conjugation, Biotin and Streptavidin, Enzyme-Linked Immunosorbent Assay (ELISA), Kinases, Horseradish Peroxidase (HRP) and Poly-HRP, Immunofluorescence, Oxidases, Secondary Antibodies, Serology Assays for Antibody Detection, Western Blotting Assays
CAS 119171-73-2 | Molecular weight 257.24 | Excitation (nm) 571 | Emission (nm) 584 |
Our Amplite® ADHP is chemically same to Amplex™ Red (Amplex™ Red is the trademark of Invitrogen). It is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 388.742 µL | 1.944 mL | 3.887 mL | 19.437 mL | 38.874 mL |
5 mM | 77.748 µL | 388.742 µL | 777.484 µL | 3.887 mL | 7.775 mL |
10 mM | 38.874 µL | 194.371 µL | 388.742 µL | 1.944 mL | 3.887 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. Chemical structure for Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*

Figure 2. Radiation increases MPO enzymatic activity and MPO carrying leukocytes in the tumor microenvironment. (A) Bar graphs showing increased MPO enzymatic activity measured as relative fluorescence units (RFU)/sec/mg of protein in irradiated (IR) tumors compared to non-irradiated (no IR) tumors. CL brain: contra-lateral normal cerebral hemisphere devoid of tumor. n = 4/group (B) Flow cytometry dot plots showing neutrophils and lymphocyte antigen 6C (Ly6C)high monocytes/macrophage (Mo/MΦ) in irradiated (IR) and non-irradiated (no IR) tumors. SSC: side scatter, Ly6G: lymphocyte antigen 6G. (C) Flow cytometry analysis showing neutrophil and Ly-6Chigh monocyte/macrophages (Mo/MΦ) or monocyte (Mo) cell numbers out of total leukocytes defined as CD45+ cells. Cells were isolated from brain tumor (left), peripheral blood (middle) and bone marrow (right) of irradiated (IR) and non-irradiated (no IR) mice (n = 4-5/group). Mice were irradiated 14 days after tumor injections and flow cytometry was performed 1 week after radiation. MPO activity of captured MPO was assessed with a 10-acetyl-3,7-dihydroxyphenoxazine (ADHP, AAT Bioquest). Data plotted as mean ± SEM. *p < 0.05. Source: Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy by Ali et. al., Neoplasia. April 2022.
Citations
View all 20 citations: Citation Explorer
Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy
Authors: Ali, Muhammad and Fulci, Giulia and Grigalavicius, Mantas and Pulli, Benjamin and Li, Anning and Wojtkiewicz, Gregory R and Wang, Cuihua and Hsieh, Kevin Li-Chun and Linnoila, Jenny J and Theodossiou, Theodossis A and others,
Journal: Neoplasia (2022): 100779
Authors: Ali, Muhammad and Fulci, Giulia and Grigalavicius, Mantas and Pulli, Benjamin and Li, Anning and Wojtkiewicz, Gregory R and Wang, Cuihua and Hsieh, Kevin Li-Chun and Linnoila, Jenny J and Theodossiou, Theodossis A and others,
Journal: Neoplasia (2022): 100779
Myeloperoxidase modulates inflammation in generalized pustular psoriasis and additional rare pustular skin diseases
Authors: Haskamp, Stefan and Bruns, Heiko and Hahn, Madelaine and Hoffmann, Markus and Gregor, Anne and L{\"o}hr, Sabine and Hahn, Jonas and Schauer, Christine and Ringer, Mark and Flamann, Cindy and others,
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Journal: Microorganisms (2020): 1039
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Multimodal Molecular Imaging Demonstrates Myeloperoxidase Regulation of Matrix Metalloproteinase Activity in Neuroinflammation
Authors: Zhang, Yinian and Dong, Huateng and Seeburg, Daniel P and Wojtkiewicz, Gregory R and Waterman, Peter and Pulli, Benjamin and Forghani, Reza and Ali, Muhammad and Iwamoto, Yoshiko and Swirski, Filip K and others, undefined
Journal: Molecular neurobiology (2018): 1--9
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Spinal cord inflammation: molecular imaging after thoracic aortic ischemia reperfusion injury
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: Radiology (2017): 202--211
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: Radiology (2017): 202--211
Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase
Authors: Milton, Amber
Journal: (2017)
Authors: Milton, Amber
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Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical Chemistry (2017)
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical Chemistry (2017)
Patterned photonic nitrocellulose for pseudopaper ELISA
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical chemistry (2017): 7727--7733
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical chemistry (2017): 7727--7733
Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase
Authors: Milton, Amber
Journal: (2017)
Authors: Milton, Amber
Journal: (2017)
Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: Radiology (2016): 152222
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: Radiology (2016): 152222
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Application notes
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Ameliorative Effect of Novel Vitamin Formula with Herbal Extracts on Scopolamine-Induced Alzheimer's Disease
An Increase in Plasma Homovanillic Acid with Cocoa Extract Consumption Is Associated with the Alleviation of Depressive Symptoms in Overweight or Obese Adults
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Ameliorative Effect of Novel Vitamin Formula with Herbal Extracts on Scopolamine-Induced Alzheimer's Disease
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Attenuation of lysyl oxidase and collagen gene expression in keratoconus patient corneal epithelium corresponds to disease severity