AAT Bioquest

Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2*

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Radiation increases MPO enzymatic activity and MPO carrying leukocytes in the tumor microenvironment. (A) Bar graphs showing increased MPO enzymatic activity measured as relative fluorescence units (RFU)/sec/mg of protein in irradiated (IR) tumors compared to non-irradiated (no IR) tumors. CL brain: contra-lateral normal cerebral hemisphere devoid of tumor. n = 4/group (B) Flow cytometry dot plots showing neutrophils and lymphocyte antigen 6C (Ly6C)high monocytes/macrophage (Mo/MΦ) in irradiated (IR) and non-irradiated (no IR) tumors. SSC: side scatter, Ly6G: lymphocyte antigen 6G. (C) Flow cytometry analysis showing neutrophil and Ly-6Chigh monocyte/macrophages (Mo/MΦ) or monocyte (Mo) cell numbers out of total leukocytes defined as CD45+ cells. Cells were isolated from brain tumor (left), peripheral blood (middle) and bone marrow (right) of irradiated (IR) and non-irradiated (no IR) mice (n = 4-5/group). Mice were irradiated 14 days after tumor injections and flow cytometry was performed 1 week after radiation. MPO activity of captured MPO was assessed with a 10-acetyl-3,7-dihydroxyphenoxazine (ADHP, AAT Bioquest). Data plotted as mean ± SEM. *p < 0.05. Source: <b>Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy</b> by Ali et. al., <em>Neoplasia</em>. April 2022.
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Physical properties
Molecular weight257.24
Spectral properties
Excitation (nm)571
Emission (nm)584
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Our Amplite® ADHP is chemically same to Amplex™ Red (Amplex™ Red is the trademark of Invitrogen). It is a sensitive fluorogenic peroxidase substrate that has much lower background than the material from other commercial vendors. ADHP generates highly fluorescent resorufin that has maximum absorption of 571 nm and maximum emission of 585 nm. Unlike other HRP substrates such as dihydrofluoresceins and dihydrorhodamines, the air-oxidation of ADHP is minimal. So far ADHP has been known as the most sensitive and stable fluorogenic probe for detecting HRP and H2O2. ADHP has been widely used to detect HRP in many immunoassays. On the other hand, ADHP can also be used to detect trace amount of H2O2. The ADHP-based H2O2 detection is at least one order of magnitude more sensitive than the commonly used scopoletin assay for H2O2. Because H2O2 is produced in many enzymatic redox reactions, ADHP can be used in coupled enzymatic reactions to detect the activity of many oxidases and/or related enzymes/substrates or cofactors such as glucose, acetylcholine and cholesterol, L-glutamate, amino acids, etc.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Amplite® ADHP [10-Acetyl-3,7-dihydroxyphenoxazine] *CAS#: 119171-73-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM388.742 µL1.944 mL3.887 mL19.437 mL38.874 mL
5 mM77.748 µL388.742 µL777.484 µL3.887 mL7.775 mL
10 mM38.874 µL194.371 µL388.742 µL1.944 mL3.887 mL

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Spectral properties

Excitation (nm)571
Emission (nm)584

Product Family

NameExcitation (nm)Emission (nm)
Amplite® IR648668
Amplite® Red571584



View all 20 citations: Citation Explorer
Myeloperoxidase exerts anti-tumor activity in glioma after radiotherapy
Authors: Ali, Muhammad and Fulci, Giulia and Grigalavicius, Mantas and Pulli, Benjamin and Li, Anning and Wojtkiewicz, Gregory R and Wang, Cuihua and Hsieh, Kevin Li-Chun and Linnoila, Jenny J and Theodossiou, Theodossis A and others,
Journal: Neoplasia (2022): 100779
C3 opsonization of anthrax bacterium and peptidoglycan supports recognition and activation of neutrophils
Authors: Popescu, Narcis I and Keshari, Ravi S and Cochran, Jackie and Coggeshall, K Mark and Lupu, Florea
Journal: Microorganisms (2020): 1039
Myeloperoxidase modulates inflammation in generalized pustular psoriasis and additional rare pustular skin diseases
Authors: Haskamp, Stefan and Bruns, Heiko and Hahn, Madelaine and Hoffmann, Markus and Gregor, Anne and L{\"o}hr, Sabine and Hahn, Jonas and Schauer, Christine and Ringer, Mark and Flamann, Cindy and others,
Journal: The American Journal of Human Genetics (2020): 527--538
Multimodal Molecular Imaging Demonstrates Myeloperoxidase Regulation of Matrix Metalloproteinase Activity in Neuroinflammation
Authors: Zhang, Yinian and Dong, Huateng and Seeburg, Daniel P and Wojtkiewicz, Gregory R and Waterman, Peter and Pulli, Benjamin and Forghani, Reza and Ali, Muhammad and Iwamoto, Yoshiko and Swirski, Filip K and others, undefined
Journal: Molecular neurobiology (2018): 1--9
Patterned photonic nitrocellulose for pseudopaper ELISA
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical chemistry (2017): 7727--7733
Spinal cord inflammation: molecular imaging after thoracic aortic ischemia reperfusion injury
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: Radiology (2017): 202--211
Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical Chemistry (2017)
Myeloperoxidase nuclear imaging for epileptogenesis
Authors: Zhang, Yinian and Seeburg, Daniel P and Pulli, Benjamin and Wojtkiewicz, Gregory R and Bure, Lionel and Atkinson, Wendy and Schob, Stefan and Iwamoto, Yoshiko and Ali, Muhammad and Zhang, Wei and others,
Journal: Radiology (2016): 822--830


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