Goat anti-rabbit IgG (H&L) Agarose
| Overview |  SDSProtocol |
See also: Anti-Rabbit Secondary Antibodies, Antibodies and Proteomics, Antibody and Protein Labeling, Bioconjugation, iFluor Secondary Reagents, Immunohistochemistry (IHC), Western Blotting Assays
Molecular weight 150000 |
Agarose conjugated antibodies are designed for the rapid and efficient collection of antibodies and proteins, including recombinant fusion proteins, from a complex protein mixture (see protocol). Antibody affinity gels are useful for selection of immunoglobulins and immunoprecipitation of antigens from sera, antisera, ascitic fluid or culture fluid, bacterial and mammalian cell lysates and fusion protein preparations.
Calculators
Common stock solution preparation
Table 1. Volume of appropriate solvent needed to reconstitute specific mass of Goat anti-rabbit IgG (H&L) Agarose to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 666.667 nL | 3.333 µL | 6.667 µL | 33.333 µL | 66.667 µL |
| 5 mM | 133.333 nL | 666.667 nL | 1.333 µL | 6.667 µL | 13.333 µL |
| 10 mM | 66.667 nL | 333.333 nL | 666.667 nL | 3.333 µL | 6.667 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) | Correction Factor (260 nm) |
| FITC goat anti-rabbit IgG (H+L) | 491 | 516 | 73000 | 0.92 | 0.254 | - |
| Cy3® goat anti-rabbit IgG (H+L) | 555 | 569 | 1500001 | 0.151 | 0.073 | 0.07 |
| Cy5® goat anti-rabbit IgG (H+L) | 651 | 670 | 2500001 | 0.271, 0.42 | 0.03 | 0.02 |
| Cy7® goat anti-rabbit IgG (H+L) | 756 | 779 | 250000 | 0.3 | 0.036 | 0.05 |
| FITC goat anti-rabbit IgG (H+L) *Cross Adsorbed* | 491 | 516 | 73000 | 0.92 | 0.254 | - |
| Cy3® goat anti-rabbit IgG (H+L) *Cross Adsorbed* | 555 | 569 | 1500001 | 0.151 | 0.073 | 0.07 |
| Cy5® goat anti-rabbit IgG (H+L) *Cross Adsorbed* | 651 | 670 | 2500001 | 0.271, 0.42 | 0.03 | 0.02 |
| Cy7® goat anti-rabbit IgG (H+L) *Cross Adsorbed* | 756 | 779 | 250000 | 0.3 | 0.036 | 0.05 |
| Biotinylated goat anti-rabbit IgG (H+L) | - | - | - | - | - | - |
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Images
Figure 1. Agarose conjugated antibodies are designed for the rapid and efficient collection of antibodies from a complex protein mixture. Antibody affinity gels are useful for selection of immunoglobulins and immunoprecipitation of antigens from sera, antisera, ascitic fluid or culture fluid, bacterial and mammalian cell lysates and fusion protein preparations.
References
View all 4 references: Citation Explorer
Transient conformational modification of immunoglobulin G during purification by protein A affinity chromatography
Authors: Gagnon, P.; Nian, R.; Leong, D.; Hoi, A.
Journal: J Chromatogr A (2015): 136-42
Authors: Gagnon, P.; Nian, R.; Leong, D.; Hoi, A.
Journal: J Chromatogr A (2015): 136-42
Purification of caveolae by affinity two-phase partitioning using biotinylated antibodies and NeutrAvidin-dextran
Authors: Barinaga-Rementeria Ramirez, I.; Abedinpour, P.; Jergil, B.
Journal: Anal Biochem (2004): 17-26
Authors: Barinaga-Rementeria Ramirez, I.; Abedinpour, P.; Jergil, B.
Journal: Anal Biochem (2004): 17-26
Expression screen by enzyme-linked immunofiltration assay designed for high-throughput purification of affinity-tagged proteins
Authors: Kery, V.; Savage, J. R.; Widjaja, K.; Blake, B. K.; Conklin, D. R.; Ho, Y. S.; Long, X.; von Rechenberg, M.; Zarembinski, T. I.; Boniface, J. J.
Journal: Anal Biochem (2003): 255-8
Authors: Kery, V.; Savage, J. R.; Widjaja, K.; Blake, B. K.; Conklin, D. R.; Ho, Y. S.; Long, X.; von Rechenberg, M.; Zarembinski, T. I.; Boniface, J. J.
Journal: Anal Biochem (2003): 255-8
Chromogranin from normal human adrenal glands: purification by monoclonal antibody affinity chromatography and partial N-terminal amino acid sequence
Authors: Wilson, B. S.; Phan, S. H.; Lloyd, R. V.
Journal: Regul Pept (1986): 207-23
Authors: Wilson, B. S.; Phan, S. H.; Lloyd, R. V.
Journal: Regul Pept (1986): 207-23
FAQ
I am performing an ELISA using biotinylated goat secondary antibodies, can you recommend a working concentration?
What are the subclasses of immunoglobulins?
Are there any alternatives to BrdU (Bromodeoxyuridine)?
Are there any alternatives to Cy5?
Are there any alternatives to indocyanine green (ICG)?