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Goat anti-rabbit IgG (H&L) Agarose

Agarose conjugated antibodies are designed for the rapid and efficient collection of antibodies from a complex protein mixture. Antibody affinity gels are useful for selection of immunoglobulins and immunoprecipitation of antigens from sera, antisera, ascitic fluid or culture fluid, bacterial and mammalian cell lysates and fusion protein preparations.
Agarose conjugated antibodies are designed for the rapid and efficient collection of antibodies from a complex protein mixture. Antibody affinity gels are useful for selection of immunoglobulins and immunoprecipitation of antigens from sera, antisera, ascitic fluid or culture fluid, bacterial and mammalian cell lysates and fusion protein preparations.
Ordering information
Price ()
Catalog Number55015
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight150000
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C)
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
150000
Agarose conjugated antibodies are designed for the rapid and efficient collection of antibodies and proteins, including recombinant fusion proteins, from a complex protein mixture (see protocol). Antibody affinity gels are useful for selection of immunoglobulins and immunoprecipitation of antigens from sera, antisera, ascitic fluid or culture fluid, bacterial and mammalian cell lysates and fusion protein preparations.

Calculators


Common stock solution preparation

Table 1. Volume of appropriate solvent needed to reconstitute specific mass of Goat anti-rabbit IgG (H&L) Agarose to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM666.667 nL3.333 µL6.667 µL33.333 µL66.667 µL
5 mM133.333 nL666.667 nL1.333 µL6.667 µL13.333 µL
10 mM66.667 nL333.333 nL666.667 nL3.333 µL6.667 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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References


View all 4 references: Citation Explorer
Transient conformational modification of immunoglobulin G during purification by protein A affinity chromatography
Authors: Gagnon, P.; Nian, R.; Leong, D.; Hoi, A.
Journal: J Chromatogr A (2015): 136-42
Purification of caveolae by affinity two-phase partitioning using biotinylated antibodies and NeutrAvidin-dextran
Authors: Barinaga-Rementeria Ramirez, I.; Abedinpour, P.; Jergil, B.
Journal: Anal Biochem (2004): 17-26
Expression screen by enzyme-linked immunofiltration assay designed for high-throughput purification of affinity-tagged proteins
Authors: Kery, V.; Savage, J. R.; Widjaja, K.; Blake, B. K.; Conklin, D. R.; Ho, Y. S.; Long, X.; von Rechenberg, M.; Zarembinski, T. I.; Boniface, J. J.
Journal: Anal Biochem (2003): 255-8
Chromogranin from normal human adrenal glands: purification by monoclonal antibody affinity chromatography and partial N-terminal amino acid sequence
Authors: Wilson, B. S.; Phan, S. H.; Lloyd, R. V.
Journal: Regul Pept (1986): 207-23