Luciferins are a powerful and versatile class of chemiluminescent substrates that can emit light upon oxidation catalyzed by various types of luciferases. This bioluminescent system is used in various applications and is known for its high sensitivity, background-free luminescence, speed, and affordability. The luciferin-luciferase reaction is often utilized for detecting low level gene expression and cellular ATP activity, for in vivo imaging and in various reporter assays. AAT Bioquest provides an array of luciferase substrates and reporter gene assays optimized to yield consistent results with high sensitivity over a wide dynamic range.
The chemical structure of D-Luciferin, potassium salt CAS#: 115144-35-9 (Cat# 12507).
Allophycocyanin (APC) is a phycobiliprotein that exhibits a far-red fluorescence with a high absorptivity and quantum efficiency. Its high water solubility, large Stokes shift and quench-resistant properties make it effective in fluorescence-based applications (primarily in flow cytometry, immunoassays, and FRET/tandem dyes). AAT Bioquest offers a broad range of APC products including secondary antibody and streptavidin conjugates, tandem dyes, and APC-labeling kits.
Absorption and emission spectrum of APC (Cat#2554). Excitations at 594 nm (Orange laser line) and 633 nm (Red laser line). APC read with emission filter Cy5® (Red band).
ReadiUse™ TCEP removal buffer
Tris(2-carboxyethyl)phosphine (TCEP) is a potent disulfide reducing agent frequently utilized in biochemistry and molecular biology applications. TCEP is used as a reducing agent to break disulfide bonds between proteins for gel electrophoresis. It is used in the tissue homogenization process for RNA isolation, and in site-specific conjugation of protein or antibody cysteine residues with maleimide derivative labels (e.g., iFluor® 488 maleimide). Due to its potent reactivity, the removal of TCEP is essential in most downstream applications. AAT Bioquest's ReadiUse™ TCEP removal buffer effectively eliminates residual TCEP in less than one hour with a single mixing step.
Reduction of Ellman's reagent by TCEP after treatment with/without ReadiUse™ TCEP removal buffer (Cat #5540). TCEP was treated with/without ReadiUse™ TCEP removal buffer for 10 min. Mixture was then incubated with Ellman's reagent for 30 min.