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ReadiUse™ TCEP removal solution

Reduction of Ellman’s reagent by TCEP after it is treated with/without ReadiUse™ TCEP removal solution(Cat #5540).  TCEP was treated with/without ReadiUse™ TCEP removal solution for 10min, and then the mixture was incubated with Ellman’s reagent for 30min.  Absorbance at 420nm was monitored using SpectraMax microplate reader.
Reduction of Ellman’s reagent by TCEP after it is treated with/without ReadiUse™ TCEP removal solution(Cat #5540).  TCEP was treated with/without ReadiUse™ TCEP removal solution for 10min, and then the mixture was incubated with Ellman’s reagent for 30min.  Absorbance at 420nm was monitored using SpectraMax microplate reader.
Ordering information
Price ()
Catalog Number5540
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weightN/A
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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OverviewpdfSDSpdfProtocol


Molecular weight
N/A
TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins. Compared to the other two most common reducing agents, i.e., dithiothreitol (DTT) and 2-mercaptoethanol, TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent, more hydrophilic, and more resistant to oxidation in air. TCEP is also used in the tissue homogenization process for RNA isolation. However, there are numerous reports that TCEP is not compatible with downstream assays that often have components reactive to TCEP. Our ReadiUse™ TCEP removing buffer can be effectively used for eliminating residual TCEP. It is a preformatted 2M solution that can be simply added to a solution that contains residual TCEP.

References


View all 31 references: Citation Explorer
Palladium(II) complexes with tris(2-carboxyethyl)phosphine, structure, reactions and cytostatic activity
Authors: Pruchnik H, Lis T, Latocha M, Zielinska A, Pruchnik FP.
Journal: J Inorg Biochem (2016): 14
Chemical Etching of Bovine Serum Albumin-Protected Au25 Nanoclusters for Label-Free and Separation-Free Ratiometric Fluorescent Detection of Tris(2-carboxyethyl)phosphine
Authors: Shu T, Wang J, Su L, Zhang X.
Journal: Anal Chem (2016): 11193
A high-throughput hydrophilic interaction liquid chromatography coupled with a charged aerosol detector method to assess trisulfides in IgG1 monoclonal antibodies using tris(2-carboxyethyl)phosphine reaction products: Tris(2-carboxyethyl)phosphine-oxide an
Authors: Cornell C, Karanjit A, Chen Y, Jacobson F.
Journal: J Chromatogr A (2016): 107
The Role of Tris(2-carboxyethyl)phosphine Reducing Agent in the Controlled Formation of alpha,omega-Alkanedithiols Monolayers on Au(111) with Monocoordinated and Bicoordinated Configurations
Authors: Euti EM, Velez-Romero P, Leiva EP, Macagno VA, Paredes-Olivera PA, Patrito EM, Cometto FP.
Journal: Langmuir (2016): 9428
Dissociation and reduction of covalent beta-lactoglobulin-quinone adducts by dithiothreitol, tris(2-carboxyethyl)phosphine, or sodium sulfite
Authors: Jongberg S, Lund MN, Otte J.
Journal: Anal Biochem (2015): 40
Direct measurement of active thiol metabolite levels of clopidogrel in human plasma using tris(2-carboxyethyl)phosphine as a reducing agent by LC-MS/MS
Authors: Park JB, Bae SH, Jang SM, Noh WJ, Hong JH, Yoon KD, Kang HC, Bae SK.
Journal: J Sep Sci (2013): 2306
Loss of cationic peptides with agarose gel-immobilized tris[2-carboxyethyl]phosphine (TCEP)
Authors: Shriver-Lake LC, North SH, Rowe Taitt C.
Journal: Biotechniques (2013): 292
Microwave-mediated reduction of disulfide bridges with supported (tris(2-carboxyethyl)phosphine) as resin-bound reducing agent
Authors: Miralles G, Verdie P, Puget K, Maurras A, Martinez J, Subra G.
Journal: ACS Comb Sci (2013): 169
Tris-(2-carboxyethyl) phosphine significantly promotes the reaction of cisplatin with Sp1 zinc finger protein
Authors: Chen S, Jiang H, Wei K, Liu Y.
Journal: Chem Commun (Camb) (2013): 1226
On-line cleavage of disulfide bonds by soluble and immobilized tris-(2-carboxyethyl)phosphine using sequential injection analysis
Authors: Tzanavaras PD, Mitani C, Anthemidis A, Themelis DG.
Journal: Talanta (2012): 21