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ReadiUse™ TCEP removal solution
TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins. Compared to the other two most common reducing agents, i.e., dithiothreitol (DTT) and 2-mercaptoethanol, TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent, more hydrophilic, and more resistant to oxidation in air. TCEP is also used in the tissue homogenization process for RNA isolation. However, there are numerous reports that TCEP is not compatible with downstream assays that often have components reactive to TCEP. Our ReadiUse™ TCEP removing buffer can be effectively used for eliminating residual TCEP. It is a preformatted 2M solution that can be simply added to a solution that contains residual TCEP.
<p>Reduction of Ellman&rsquo;s reagent by TCEP after it is treated with/without ReadiUse&trade; TCEP removal solution(Cat #5540).&nbsp; TCEP was treated with/without ReadiUse&trade; TCEP removal solution for 10min, and then the mixture was incubated with Ellman&rsquo;s reagent for 30min.&nbsp; Absorbance at 420nm was monitored using SpectraMax microplate reader.</p>
<p>Reduction of Ellman&rsquo;s reagent by TCEP after it is treated with/without ReadiUse&trade; TCEP removal solution(Cat #5540).&nbsp; TCEP was treated with/without ReadiUse&trade; TCEP removal solution for 10min, and then the mixture was incubated with Ellman&rsquo;s reagent for 30min.&nbsp; Absorbance at 420nm was monitored using SpectraMax microplate reader.</p>
CatalogSize
Price
Quantity
55401 ml
Price
 
Physical properties

Molecular weightN/A
SolventWater
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRoom temperature (10-25 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 4, 2025