Name: ReadiUse™ TCEP removal solution
Brand: AAT Bioquest
SKU: 5540
Price: 237 USD
Availability: InStock

ReadiUse™ TCEP removal solution

ReadiUse™ TCEP removal solution is a reagent for eliminating residual TCEP from solutions prior to downstream assays.

Function: Effectively removes residual TCEP from solution.
Formulation: Supplied as a 2 M preformatted solution.
Incubation: Incubate 30–60 minutes at room temperature.
Application: Helps eliminate TCEP before assays with TCEP-reactive components.

<p>Reduction of Ellman’s reagent by TCEP after it is treated with/without ReadiUse™ TCEP removal solution(Cat #5540).  TCEP was treated with/without ReadiUse™ TCEP removal solution for 10min, and then the mixture was incubated with Ellman’s reagent for 30min.  Absorbance at 420nm was monitored using SpectraMax microplate reader.</p>

Protocol

SDS

COA

Catalog	Size	Price	Quantity
5540	1 ml	Price

Overview

TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins. Compared to the other two most common reducing agents, i.e., dithiothreitol (DTT) and 2-mercaptoethanol, TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent, more hydrophilic, and more resistant to oxidation in air. TCEP is also used in the tissue homogenization process for RNA isolation. However, there are numerous reports that TCEP is not compatible with downstream assays that often have components reactive to TCEP. Our ReadiUse™ TCEP removing buffer can be effectively used for eliminating residual TCEP. It is a preformatted 2M solution that can be simply added to a solution that contains residual TCEP.

References

View all 31 references: Citation Explorer

Palladium(II) complexes with tris(2-carboxyethyl)phosphine, structure, reactions and cytostatic activity

Authors:

Pruchnik H, Lis T, Latocha M, Zielinska A, Pruchnik FP.

Journal:

J Inorg Biochem (2016): 14

Chemical Etching of Bovine Serum Albumin-Protected Au25 Nanoclusters for Label-Free and Separation-Free Ratiometric Fluorescent Detection of Tris(2-carboxyethyl)phosphine

Authors:

Shu T, Wang J, Su L, Zhang X.

Journal:

Anal Chem (2016): 11193

A high-throughput hydrophilic interaction liquid chromatography coupled with a charged aerosol detector method to assess trisulfides in IgG1 monoclonal antibodies using tris(2-carboxyethyl)phosphine reaction products: Tris(2-carboxyethyl)phosphine-oxide an

Authors:

Cornell C, Karanjit A, Chen Y, Jacobson F.

Journal:

J Chromatogr A (2016): 107

The Role of Tris(2-carboxyethyl)phosphine Reducing Agent in the Controlled Formation of alpha,omega-Alkanedithiols Monolayers on Au(111) with Monocoordinated and Bicoordinated Configurations

Authors:

Euti EM, Velez-Romero P, Leiva EP, Macagno VA, Paredes-Olivera PA, Patrito EM, Cometto FP.

Journal:

Langmuir (2016): 9428

Dissociation and reduction of covalent beta-lactoglobulin-quinone adducts by dithiothreitol, tris(2-carboxyethyl)phosphine, or sodium sulfite

Authors:

Jongberg S, Lund MN, Otte J.

Journal:

Anal Biochem (2015): 40

Physical properties

Molecular weight	N/A
Solvent	Water

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Room temperature (10-25 °C); Minimize light exposure

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Page updated on October 14, 2026