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ReadiUse™ TCEP removal solution
TCEP is a common reducing reagent widely used to break disulfide bonds within and between proteins. Compared to the other two most common reducing agents, i.e., dithiothreitol (DTT) and 2-mercaptoethanol, TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent, more hydrophilic, and more resistant to oxidation in air. TCEP is also used in the tissue homogenization process for RNA isolation. However, there are numerous reports that TCEP is not compatible with downstream assays that often have components reactive to TCEP. Our ReadiUse™ TCEP removing buffer can be effectively used for eliminating residual TCEP. It is a preformatted 2M solution that can be simply added to a solution that contains residual TCEP.
Example protocol
AT A GLANCE
Important Note
Warm up the ReadiLink™ TCEP removal buffer at room temperature before using.
SAMPLE EXPERIMENTAL PROTOCOL
Estimate the TCEP amount to be removed.
Add the ReadiLink™ TCEP removal buffer to a solution that contains residual TCEP at molar ratio of 100 : 1 TCEP Removal Buffer/TCEP (to be removed).
Incubate the solution at room temperature from 30 to 60 minutes.
The solution is now ready to use for desired downstream assays.
References
View all 31 references: Citation Explorer
Palladium(II) complexes with tris(2-carboxyethyl)phosphine, structure, reactions and cytostatic activity
Authors: Pruchnik H, Lis T, Latocha M, Zielinska A, Pruchnik FP.
Journal: J Inorg Biochem (2016): 14
Authors: Pruchnik H, Lis T, Latocha M, Zielinska A, Pruchnik FP.
Journal: J Inorg Biochem (2016): 14
Chemical Etching of Bovine Serum Albumin-Protected Au25 Nanoclusters for Label-Free and Separation-Free Ratiometric Fluorescent Detection of Tris(2-carboxyethyl)phosphine
Authors: Shu T, Wang J, Su L, Zhang X.
Journal: Anal Chem (2016): 11193
Authors: Shu T, Wang J, Su L, Zhang X.
Journal: Anal Chem (2016): 11193
A high-throughput hydrophilic interaction liquid chromatography coupled with a charged aerosol detector method to assess trisulfides in IgG1 monoclonal antibodies using tris(2-carboxyethyl)phosphine reaction products: Tris(2-carboxyethyl)phosphine-oxide an
Authors: Cornell C, Karanjit A, Chen Y, Jacobson F.
Journal: J Chromatogr A (2016): 107
Authors: Cornell C, Karanjit A, Chen Y, Jacobson F.
Journal: J Chromatogr A (2016): 107
The Role of Tris(2-carboxyethyl)phosphine Reducing Agent in the Controlled Formation of alpha,omega-Alkanedithiols Monolayers on Au(111) with Monocoordinated and Bicoordinated Configurations
Authors: Euti EM, Velez-Romero P, Leiva EP, Macagno VA, Paredes-Olivera PA, Patrito EM, Cometto FP.
Journal: Langmuir (2016): 9428
Authors: Euti EM, Velez-Romero P, Leiva EP, Macagno VA, Paredes-Olivera PA, Patrito EM, Cometto FP.
Journal: Langmuir (2016): 9428
Dissociation and reduction of covalent beta-lactoglobulin-quinone adducts by dithiothreitol, tris(2-carboxyethyl)phosphine, or sodium sulfite
Authors: Jongberg S, Lund MN, Otte J.
Journal: Anal Biochem (2015): 40
Authors: Jongberg S, Lund MN, Otte J.
Journal: Anal Biochem (2015): 40
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