Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

ReadiUse™ 10% Triton X-100 *Hydrogen Peroxide-and Carbonyl-Free*

Chemical structure for ReadiUse™ 10% Triton X-100 *Hydrogen Peroxide-and Carbonyl-Free*.
Chemical structure for ReadiUse™ 10% Triton X-100 *Hydrogen Peroxide-and Carbonyl-Free*.
Ordering information
Price ()
Catalog Number60012
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventWater
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageRoom temperature (10-25 °C)
Related products
ReadiUse™ Preactivated APC-iFluor® 700 Tandem
ReadiUse™ Preactivated APC-iFluor® 750 Tandem
ReadiUse™ Preactivated PE-iFluor® 647 Tandem
ReadiUse™ Preactivated PE-iFluor® 750 Tandem
ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
ReadiUse™ hydrogen peroxide solution *50 mM calibrated and stabilized solution*
ReadiUse™ Preactivated HRP NHS ester
ReadiUse™ NADPH Regenerating Kit
ReadiUse™ NADP Regenerating Kit
ReadiUse™ TCA Deproteinization Sample Preparation Kit
ReadiUse™ microscope mounting solution
ReadiUse™ 4% formaldehyde fixation solution
ReadiUse™ mammalian cell lysis buffer *5X*
ReadiUse™ probenecid, sodium salt *Water-soluble*
ReadiUse™ probenecid *25 mM stabilized aqueous solution*
ReadiUse™ CFSE [5-(and 6)-Carboxyfluorescein diacetate, succinimidyl ester] *CAS 150347-59-4*
ReadiUse™ Preactivated PE
ReadiUse™ Preactivated APC
ReadiUse™ Preactivated PE-Cy5 Tandem
ReadiUse™ Preactivated PE-Cy5.5 Tandem
ReadiUse™ Preactivated PE-Cy7 Tandem
ReadiUse™ Preactivated PE-Texas Red Tandem
ReadiUse™ Preactivated APC-Cy5.5 Tandem
ReadiUse™ Preactivated APC-Cy7 Tandem
ReadiUse™ TCEP removal solution
ReadiUse™ WST-8 *50 mM aqueous solution*
ReadiUse™ Rapid Luminometric ATP Assay Kit
ReadiUse™ PE [R-Phycoerythrin] *Ammonium Sulfate-Free*
ReadiUse™ CL-APC [Cross linked-Allophycocyanin] *Ammonium Sulfate-Free*
ReadiUse™ Preactivated PE-iFluor® 594 Tandem
ReadiUse™ Cell Detaching Buffer
ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*
ReadiUse™ Staurosporine * 1 mM DMSO stock solution*
ReadiUse™ Bio-Gel P-6 spin column
ReadiUse™ bacterial cell lysis buffer *5X*
ReadiUse™ Preactivated PE-iFluor® 700 Tandem
ReadiUse™ dNTP Mix *10 mM*
ReadiUse™ Viral RNA Lysis Buffer
ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer
ReadiUse™ Preactivated PerCP
ReadiUse™ Preactivated PerCP-Cy5.5 Tandem
ReadiUse™ Preactivated PE-iFluor® 660 Tandem
ReadiUse™ 10KD Spin Filter
ReadiUse™ Preactivated APC-iFluor® 800 Tandem
ReadiUse™ 1 Kb Plus DNA Ladder
ReadiUse™ GeneRuler 1 kb DNA Ladder
ReadiUse™ 100 bp DNA Ladder
ReadiUse™ ddNTP Terminator Mix *10 mM*
ReadiUse™ dNTP Mix Set *10 mM PCR Grade*
ReadiUse™ Preactivated HRP maleimide
ReadiUse™ Stayright™ Purple *HRP Chromogen Premixed with Hydrogen Peroxide*
ReadiUse™ Disposable PD-10 Desalting Column
ReadiUse™ Preactivated APC Maleimide [Activated Allophycocyanin]
ReadiUse™ Preactivated PE Maleimide [Activated R-Phycoerythrin]
ReadiUse™ 6-Color Human TBNK Antibody Kit *Dry Reagent Format*
ReadiUse™ MTS Reagent *50 mM aqueous solution*
ReadiUse™ Preactivated PE-Texas Red Maleimide
ReadiUse™ Preactivated PE-Cy5 Maleimide
ReadiUse™ Preactivated PE-Cy5.5 Maleimide
ReadiUse™ Preactivated PE-Cy7 Maleimide
ReadiUse™ Preactivated APC-AF700 Maleimide
ReadiUse™ Preactivated APC-Cy5.5 Maleimide
ReadiUse™ Preactivated APC-Cy7 Maleimide
ReadiUse™ Preactivated PerCP Maleimide
ReadiUse™ Preactivated PerCP-Cy5.5 Maleimide
ReadiUse™ Preactivated PerCP-Cy7 Maleimide
Show More (67)

OverviewpdfSDSpdfProtocol


Triton™ X-100 is a common non-ionic mild surfactant, which is often routinely utilized to lyse cells, extract proteins and cellular organelles, permeabilize the living cell membranes and solubilize proteins. However, the polyoxyethylene group is subject to oxidation to yield several kinds of hydroperoxides. ReadiUse™ 10% Triton X-100 is a highly purified Triton™ X-100 detergent stabilized as a 10% solution (w/v), free of DNase, RNase, Protease, and peroxides.

Example protocol


AT A GLANCE

Specifications:

Concentration: 10% in Cell Culture Water
No detectable level of DNase, RNase, Protease and peroxides

Applications:

Triton X-100 is most frequently used as a component in cell lysis buffer or other buffers to extract and solubilize proteins.

Examples of cell lysis buffer recipe (to extract cytoskeletal proteins):
10 mM PBS, pH 7.4
1 mM EDTA
1% Triton X-100

SAMPLE EXPERIMENTAL PROTOCOL

Protocols for preparation of cell lysate:

  1. Wash attached cells in cell culture dish with ice-cold PBS.

  2. Aspirate PBS, add ice-cold lysis buffer
    1 mL: 100 mm dish or 150 cm2 flask;
    5 mL: 60 mm dish or 75 cm2 flask

  3. Scrape adherent cells off the dish, and gently transfer the cell suspension into a pre-cooled microcentrifuge tube.

  4. Maintain constant agitation for 30 minutes at 4 °C.

  5. Centrifuge in a microcentrifuge at 4 °C.

  6. Gently collect the supernatant and store in a fresh tube on ice, the cell lysate is ready for use.