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ReadiUse™ 10KD Spin Filter

ReadiUse™ 10KD Spin Filters are disposable ultrafiltration devices for the concentration of biological samples such as antibodies. Maximum initial sample volume is ~500 ul. They can be used in either a swing bucket or fixed angle rotors accepting 2.0 ml centrifuge tube at around 10000 g.
ReadiUse™ 10KD Spin Filters are disposable ultrafiltration devices for the concentration of biological samples such as antibodies. Maximum initial sample volume is ~500 ul. They can be used in either a swing bucket or fixed angle rotors accepting 2.0 ml centrifuge tube at around 10000 g.
Ordering information
Price ()
Catalog Number60502
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRoom temperature (10-25 °C)
UNSPSC12171501
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OverviewpdfProtocol


ReadiUse™ 10KD Spin Filter is a disposable ultrafiltration device for the concentration of biological samples such as antibodies. Maximum initial sample volume is ~500 uL. It can be used in either a swing bucket or fixed angle rotors accepting 2.0 mL centrifuge tube at around 10000 g. The dead stop volume is ~20-25 µL. The vertical membrane design and thin channel filtration chamber minimizes membrane fouling and provides high speed concentration, even laden solutions. It is specifically designed with low internal surface and membrane area to achieve superior recovery from dilute solutions.

Example protocol


AT A GLANCE

Overview
  1. Polypropylene housing and polyethersulfone (PES) membrane
  2. MWCO: 10K
  3. Sample Volume: 25 µL-500 µL
  4. High sample recovery
  5. Application: Protein concentration,  desalting, buffer exchange, and deproteinization of biological samples
  6. Maximum Centrifugal Force: 14,000 g
  7. Recommended Centrifugal Force: 5,000 ∼ 10,000 g 

SAMPLE EXPERIMENTAL PROTOCOL

Concentrate volume
Add protein sample to the filter, centrifuge at 8,000 ∼ 10,000 g.
Note     it takes about 10 ∼ 15 mins to concentrate from 500 µL to ~50 µL.


Desalting
Desalting, buffer exchange or diafiltration are important methods for removing salts or solvents in solutions containing biomolecules. The removal of salts or the exchange of buffers can be accomplished in the ReadiUse™ 10KD Spin Filter by concentrating the sample, then reconstituting the concentrate to the original sample volume with any desired solvent. The process of "washing out" can be repeated until the concentration of the contaminating microsolute has been sufficiently reduced.

Deproteinization of biological samples
Add cell lysates or tissue homogenates to the filter, centrifuge at ∼10,000 g for 10 ∼ 15min, collect the elution.

References


View all 50 references: Citation Explorer
Extraction of β-blockers from urine with a polymeric monolith modified with 1-allyl-3-methylimidazolium chloride in spin column format.
Authors: Mompó-Roselló, Oscar and Ribera-Castelló, Ana and Simó-Alfonso, Ernesto F and Ruiz-Angel, María José and García-Alvarez-Coque, María Celia and Herrero-Martínez, José Manuel
Journal: Talanta (2020): 120860
Spin-column micro-solid phase extraction of chlorophenols using MFU-4l metal-organic framework.
Authors: Esrafili, Ali and Ghambarian, Mahnaz and Tajik, Mohammad and Baharfar, Mahroo
Journal: Mikrochimica acta (2019): 39
Synthesis and characterization of polyamide-graphene oxide-polypyrrole electrospun nanofibers for spin-column micro solid phase extraction of parabens in milk samples.
Authors: Seidi, Shahram and Sadat Karimi, Elnaz and Rouhollahi, Ahmad and Baharfar, Mahroo and Shanehsaz, Maryam and Tajik, Mohammad
Journal: Journal of chromatography. A (2019): 25-34
Filter paper-based spin column method for cost-efficient DNA or RNA purification.
Authors: Shi, Rui and Lewis, Ramsey S and Panthee, Dilip R
Journal: PloS one (2018): e0203011
A spin column-free approach to sodium hydroxide-based glycan permethylation.
Authors: Hu, Yueming and Borges, Chad R
Journal: The Analyst (2017): 2748-2759
A rapid silica spin column-based method of RNA extraction from fruit trees for RT-PCR detection of viruses.
Authors: Yang, Fan and Wang, Guoping and Xu, Wenxing and Hong, Ni
Journal: Journal of virological methods (2017): 61-67
A Rapid Spin Column-Based Method to Enrich Pathogen Transcripts from Eukaryotic Host Cells Prior to Sequencing.
Authors: Bent, Zachary W and Poorey, Kunal and LaBauve, Annette E and Hamblin, Rachelle and Williams, Kelly P and Meagher, Robert J
Journal: PloS one (2016): e0168788
Development of a silica monolith modified with Fe3O4 nano-particles in centrifugal spin column format for the extraction of phosphorylated compounds.
Authors: Alwy, Ali and Clarke, Sarah P and Brougham, Dermot F and Twamley, Brendan and Paull, Brett and White, Blánaid and Connolly, Damian
Journal: Journal of separation science (2015): 283-90
Spin column extraction as a new sample preparation method in bioanalysis.
Authors: Namera, Akira and Saito, Takashi
Journal: Bioanalysis (2015): 2171-6
Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins.
Authors: Krenkova, Jana and Cesla, Petr and Foret, Frantisek
Journal: Electrophoresis (2015): 1344-8