ReadiUse™ 4% formaldehyde fixation solution

Expressions of NSE, O4 and GFAP in the experimental groups indicating NSC differentiation by fluorescence microscopy (×100). NSCs were fixed in ReadiUse™ 4% formaldehyde fixation solution (AAT Bioquest, CA, USA) for 15–20 min at room temperature, and then permeabilized with 1% (vol/vol) Triton X-100 (Invitrogen; Thermo Fisher Scientific, Inc.) in phosphate-buffered saline (PBS; Hyclone; GE Healthcare Life Sciences) for 30 min, and incubated in blocking buffer which contained 10% goat serum (Biorbyt, Cambridge, UK) for 10 min. Immunofluorescence staining performed simultaneously on each group, red (CY3) represents the expression NSE or O4 in cytoplasm, green (FITC) represents the expression of GFAP in cytoplasm, blue (Hoechst 33258) represents the nucleus. NSC, neural stem cell; NSE, enolase 2; GFAP, glial fibrillary acidic protein; O4, oligodendrocyte cell surface antigen O4; CON, blank control group; NC, negative control plasmid-transfected cells; N, normal brain tissue; HIBD, hypoxic-ischemic brain damage tissue; siNSC, β-catenin small interfering RNA-transfected cells. Source: <b>Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage</b> by Zhang, Xiaoying et.al., <em>Molecular Medicine Reports</em>, Aug. 2016

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Telephone	1-800-990-8053
Fax	1-800-609-2943
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Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Images

Figure 1. F-actin stain of CPA cells.
CPA cells were fixed with ReadiUse™ 4% formaldehyde fixation solution (Cat#20010) and then stained with Cell Navigator® F-Actin Labeling Kit (cat#22661). A: label with 1X iFluor® 488-phalloidin for 30 min only. B: Treat the cells with phalloidin for 10 min, then stain them with 1X iFluor® 488-phalloidin for 30 min.

Figure 2. Expressions of NSE, O4 and GFAP in the experimental groups indicating NSC differentiation by fluorescence microscopy (×100). NSCs were fixed in ReadiUse™ 4% formaldehyde fixation solution (AAT Bioquest, CA, USA) for 15–20 min at room temperature, and then permeabilized with 1% (vol/vol) Triton X-100 (Invitrogen; Thermo Fisher Scientific, Inc.) in phosphate-buffered saline (PBS; Hyclone; GE Healthcare Life Sciences) for 30 min, and incubated in blocking buffer which contained 10% goat serum (Biorbyt, Cambridge, UK) for 10 min. Immunofluorescence staining performed simultaneously on each group, red (CY3) represents the expression NSE or O4 in cytoplasm, green (FITC) represents the expression of GFAP in cytoplasm, blue (Hoechst 33258) represents the nucleus. NSC, neural stem cell; NSE, enolase 2; GFAP, glial fibrillary acidic protein; O4, oligodendrocyte cell surface antigen O4; CON, blank control group; NC, negative control plasmid-transfected cells; N, normal brain tissue; HIBD, hypoxic-ischemic brain damage tissue; siNSC, β-catenin small interfering RNA-transfected cells. Source: Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage by Zhang, Xiaoying et.al., Molecular Medicine Reports, Aug. 2016

Citations

View all 4 citations: Citation Explorer

Rhinacanthin C Alleviates Amyloid-β Fibrils' Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells
Authors: Chuang, Kai-An and Li, Ming-Han and Lin, Ni-Hsuan and Chang, Chih-Hsuan and Lu, I and Pan, I and Takahashi, Tomoya and Perng, Ming-Der and Wen, Shu-Fang and others, undefined
Journal: Oxidative Medicine and Cellular Longevity (2017)

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage
Authors: Zhang, Xiaoying and Zhu, Cuicui and Luo, Qiong and Dong, Jv and Liu, Lv and Li, Min and Zhu, Hongtao and Ma, Xiangping and Wang, Jun
Journal: Molecular Medicine Reports (2016): 3595--3601

Application of interleukin-22 mediates protection in experimental acetaminophen-induced acute liver injury
Authors: Scheiermann, Patrick and Bachmann, Malte and Goren, Itamar and Zwissler, Bernhard and Pfeilschifter, Josef and Mühl, Heiko
Journal: The American journal of pathology (2013): 1107--1113

Synergistic activity of αvβ3 integrins and the elastin binding protein enhance cell-matrix interactions on bioactive hydrogel surfaces
Authors: Patel, Dhaval and V, undefined and romme, Susan E and Reid, Michael E and Taite, Lakeshia J
Journal: Biomacromolecules (2012): 1420--1428

References

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