ReadiUse™ 4% formaldehyde fixation solution

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Unit Size: Cat No: Price (USD): Qty:
50 mL 20010 $75


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Additional Ordering Information
Telephone: 1-800-990-8053
Fax: 1-408-733-1304
Email: sales@aatbio.com
International: See distributors





Overview

Storage Refrigerated (2-8 °C)
Minimize light exposure
Category Assay and Sample Preparation,
Cell Imaging
All our ReadiUse™ reagents require minimal hands-on time. This 4% formaldehyde solution is a ready-to-apply format. It is widely used for fixing cells in fluorescence imaging applications.




Protocol


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This protocol only provides a guideline, and should be modified according to your specific needs.

1. Perform formaldehyde fixation: Incubate the cells with 4% formaldehyde for 10-30 minutes at room temperature.

Note: Avoid any methanol containing fixatives since methanol can damage actin during the fixation process. The preferred fixative is methanol-free formaldehyde.

 

2. Rinse the fixed cells 2-3 times in PBS.

 

3. The cells are ready for immunohistochemistry/immunofluorescence.

Note: Store the fixed cells in PBS at 4 oC.






References

Rhinacanthin C Alleviates Amyloid-β Fibrils’ Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells
Authors: Kai-An Chuang, Ming-Han Li, Ni-Hsuan Lin, Chih-Hsuan Chang, I Lu, I Pan, Tomoya Takahashi, Ming-Der Perng, Shu-Fang Wen
Journal: Oxidative Medicine and Cellular Longevity (2017)

Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage
Authors: Xiaoying Zhang, Cuicui Zhu, Qiong Luo, Jv Dong, Lv Liu, Min Li, Hongtao Zhu, Xiangping Ma, Jun Wang
Journal: Molecular Medicine Reports (2016): 3595--3601

Application of interleukin-22 mediates protection in experimental acetaminophen-induced acute liver injury
Authors: Patrick Scheiermann, Malte Bachmann, Itamar Goren, Bernhard Zwissler, Josef Pfeilschifter, Heiko Mühl
Journal: The American journal of pathology (2013): 1107--1113

Synergistic activity of αvβ3 integrins and the elastin binding protein enhance cell-matrix interactions on bioactive hydrogel surfaces
Authors: Dhaval Patel, Susan E Vandromme, Michael E Reid, Lakeshia J Taite
Journal: Biomacromolecules (2012): 1420--1428






Resources
 
Safety Data Sheet (SDS)


Certificate of Analysis