ReadiUse™ 4% formaldehyde fixation solution
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Refrigerated (2-8 °C); Minimize light exposure |
UNSPSC | 12352200 |
Related products
Overview | ![]() ![]() |
See also: Buffers and Lab Consumables
All our ReadiUse™ reagents require minimal hands-on time. This 4% formaldehyde solution is a ready-to-apply format. It is widely used for fixing cells in fluorescence imaging applications.
Images

Figure 1. F-actin stain of CPA cells.
CPA cells were fixed with ReadiUse™ 4% formaldehyde fixation solution (Cat#20010) and then stained with Cell Navigator® F-Actin Labeling Kit (cat#22661). A: label with 1X iFluor® 488-phalloidin for 30 min only. B: Treat the cells with phalloidin for 10 min, then stain them with 1X iFluor® 488-phalloidin for 30 min.
CPA cells were fixed with ReadiUse™ 4% formaldehyde fixation solution (Cat#20010) and then stained with Cell Navigator® F-Actin Labeling Kit (cat#22661). A: label with 1X iFluor® 488-phalloidin for 30 min only. B: Treat the cells with phalloidin for 10 min, then stain them with 1X iFluor® 488-phalloidin for 30 min.

Figure 2. Expressions of NSE, O4 and GFAP in the experimental groups indicating NSC differentiation by fluorescence microscopy (×100). NSCs were fixed in ReadiUse™ 4% formaldehyde fixation solution (AAT Bioquest, CA, USA) for 15–20 min at room temperature, and then permeabilized with 1% (vol/vol) Triton X-100 (Invitrogen; Thermo Fisher Scientific, Inc.) in phosphate-buffered saline (PBS; Hyclone; GE Healthcare Life Sciences) for 30 min, and incubated in blocking buffer which contained 10% goat serum (Biorbyt, Cambridge, UK) for 10 min. Immunofluorescence staining performed simultaneously on each group, red (CY3) represents the expression NSE or O4 in cytoplasm, green (FITC) represents the expression of GFAP in cytoplasm, blue (Hoechst 33258) represents the nucleus. NSC, neural stem cell; NSE, enolase 2; GFAP, glial fibrillary acidic protein; O4, oligodendrocyte cell surface antigen O4; CON, blank control group; NC, negative control plasmid-transfected cells; N, normal brain tissue; HIBD, hypoxic-ischemic brain damage tissue; siNSC, β-catenin small interfering RNA-transfected cells. Source: Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage by Zhang, Xiaoying et.al., Molecular Medicine Reports, Aug. 2016
Citations
View all 4 citations: Citation Explorer
Rhinacanthin C Alleviates Amyloid-β Fibrils' Toxicity on Neurons and Attenuates Neuroinflammation Triggered by LPS, Amyloid-β, and Interferon-γ in Glial Cells
Authors: Chuang, Kai-An and Li, Ming-Han and Lin, Ni-Hsuan and Chang, Chih-Hsuan and Lu, I and Pan, I and Takahashi, Tomoya and Perng, Ming-Der and Wen, Shu-Fang and others, undefined
Journal: Oxidative Medicine and Cellular Longevity (2017)
Authors: Chuang, Kai-An and Li, Ming-Han and Lin, Ni-Hsuan and Chang, Chih-Hsuan and Lu, I and Pan, I and Takahashi, Tomoya and Perng, Ming-Der and Wen, Shu-Fang and others, undefined
Journal: Oxidative Medicine and Cellular Longevity (2017)
Impact of siRNA targeting of β-catenin on differentiation of rat neural stem cells and gene expression of Ngn1 and BMP4 following in vitro hypoxic-ischemic brain damage
Authors: Zhang, Xiaoying and Zhu, Cuicui and Luo, Qiong and Dong, Jv and Liu, Lv and Li, Min and Zhu, Hongtao and Ma, Xiangping and Wang, Jun
Journal: Molecular Medicine Reports (2016): 3595--3601
Authors: Zhang, Xiaoying and Zhu, Cuicui and Luo, Qiong and Dong, Jv and Liu, Lv and Li, Min and Zhu, Hongtao and Ma, Xiangping and Wang, Jun
Journal: Molecular Medicine Reports (2016): 3595--3601
Application of interleukin-22 mediates protection in experimental acetaminophen-induced acute liver injury
Authors: Scheiermann, Patrick and Bachmann, Malte and Goren, Itamar and Zwissler, Bernhard and Pfeilschifter, Josef and Mühl, Heiko
Journal: The American journal of pathology (2013): 1107--1113
Authors: Scheiermann, Patrick and Bachmann, Malte and Goren, Itamar and Zwissler, Bernhard and Pfeilschifter, Josef and Mühl, Heiko
Journal: The American journal of pathology (2013): 1107--1113
Synergistic activity of αvβ3 integrins and the elastin binding protein enhance cell-matrix interactions on bioactive hydrogel surfaces
Authors: Patel, Dhaval and V, undefined and romme, Susan E and Reid, Michael E and Taite, Lakeshia J
Journal: Biomacromolecules (2012): 1420--1428
Authors: Patel, Dhaval and V, undefined and romme, Susan E and Reid, Michael E and Taite, Lakeshia J
Journal: Biomacromolecules (2012): 1420--1428
References
View all 122 references: Citation Explorer
Application of fluorescence microscopic imaging technique with self-ordered ring to residues detection of antibiotics
Authors: Liu Y, Feng JC, Li D, Cui J, Xu SF, Shen GY.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2009): 2217
Authors: Liu Y, Feng JC, Li D, Cui J, Xu SF, Shen GY.
Journal: Guang Pu Xue Yu Guang Pu Fen Xi (2009): 2217
Fluorescence lifetime-resolved imaging
Authors: Chen YC, Clegg RM.
Journal: Photosynth Res (2009): 143
Authors: Chen YC, Clegg RM.
Journal: Photosynth Res (2009): 143
Membrane lipid domains and rafts: current applications of fluorescence lifetime spectroscopy and imaging
Authors: de Almeida RF, Loura LM, Prieto M.
Journal: Chem Phys Lipids (2009): 61
Authors: de Almeida RF, Loura LM, Prieto M.
Journal: Chem Phys Lipids (2009): 61
Multiphoton microscopy and fluorescence lifetime imaging microscopy (FLIM) to monitor metastasis and the tumor microenvironment
Authors: Provenzano PP, Eliceiri KW, Keely PJ.
Journal: Clin Exp Metastasis (2009): 357
Authors: Provenzano PP, Eliceiri KW, Keely PJ.
Journal: Clin Exp Metastasis (2009): 357
Extending immunofluorescence detection limits in whole paraffin-embedded formalin fixed tissues using hyperspectral confocal fluorescence imaging
Authors: Constantinou P, Dacosta RS, Wilson BC.
Journal: J Microsc (2009): 137
Authors: Constantinou P, Dacosta RS, Wilson BC.
Journal: J Microsc (2009): 137
Multispectral fluorescence imaging
Authors: Zhou L, El-Deiry WS.
Journal: J Nucl Med (2009): 1563
Authors: Zhou L, El-Deiry WS.
Journal: J Nucl Med (2009): 1563
Invited review article: Imaging techniques for harmonic and multiphoton absorption fluorescence microscopy
Authors: Carriles R, Schafer DN, Sheetz KE, Field JJ, Cisek R, Barzda V, Sylvester AW, Squier JA.
Journal: Rev Sci Instrum (2009): 81101
Authors: Carriles R, Schafer DN, Sheetz KE, Field JJ, Cisek R, Barzda V, Sylvester AW, Squier JA.
Journal: Rev Sci Instrum (2009): 81101
Rational and precise design of novel fluorescence probes and their application to cellular response and in-vivo tumor imaging
Authors: Urano Y., undefined
Journal: Tanpakushitsu Kakusan Koso (2009): 1344
Authors: Urano Y., undefined
Journal: Tanpakushitsu Kakusan Koso (2009): 1344
Live-cell fluorescence imaging of meiotic chromosome dynamics in Schizosaccharomyces pombe
Authors: Asakawa H, Hiraoka Y.
Journal: Methods Mol Biol (2009): 53
Authors: Asakawa H, Hiraoka Y.
Journal: Methods Mol Biol (2009): 53
Lymphatic imaging in humans with near-infrared fluorescence
Authors: Rasmussen JC, Tan IC, Marshall MV, Fife CE, Sevick-Muraca EM.
Journal: Curr Opin Biotechnol (2009): 74
Authors: Rasmussen JC, Tan IC, Marshall MV, Fife CE, Sevick-Muraca EM.
Journal: Curr Opin Biotechnol (2009): 74