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ReadiUse™ bacterial cell lysis buffer *5X*

Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules. Depending upon the detergents used, either all or some membranes are lysed. ReadiUse™ reagents require minimal hands-on time. This bacterial cell lysis buffer just requires a simple 5-fold dilution. It is widely used for lyzing cells for quantifying small biological molecules such as NAD(P)/NAD(P)H measurement in bacteria.
Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules. Depending upon the detergents used, either all or some membranes are lysed. ReadiUse™ reagents require minimal hands-on time. This bacterial cell lysis buffer just requires a simple 5-fold dilution. It is widely used for lyzing cells for quantifying small biological molecules such as NAD(P)/NAD(P)H measurement in bacteria.
Ordering information
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Catalog Number24100
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageRefrigerated (2-8 °C)
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OverviewpdfSDSpdfProtocol


Cell lysis refers to the breaking down of cells, and it is often used to analyze specific cellular compositions such as proteins, lipids, nucleic acids, reporter molecules, cell signal molecules and other small biomolecules. Depending upon the detergents used, either all or some membranes are lysed. ReadiUse™ reagents require minimal hands-on time. This bacterial cell lysis buffer just requires a simple 5-fold dilution. It is widely used for lyzing cells for quantifying small biological molecules such as NAD(P)/NAD(P)H measurement in bacteria.

Example protocol


AT A GLANCE

Important
Expiration date is 6 months from the date of receipt.

PREPARATION OF WORKING SOLUTION

ReadiUse™ bacterial cell lysis buffer working solution (1X):
Add 1 mL of ReadiUse™ bacterial cell lysis buffer (5X) into 4 mL of Mili-Q water, and mix them well.

SAMPLE EXPERIMENTAL PROTOCOL

  1. Prepare bacterial cell samples by collecting bacterial cells via centrifugation (10,000 g, 0°C, 15 minutes). Use about 100 to 10 million cells/mL of ReadiUse™ lysis buffer working solution (1X), keep the treated solution at room temperature for 15 minutes.

  2. Centrifuge at 2500 rpm for 5 minutes, and use the supernatant for further biochemical analysis.

Citations


View all 36 citations: Citation Explorer
Bacteriophage Therapy Increases Complement-Mediated Lysis of Bacteria and Enhances Bacterial Clearance After Acute Lung Infection With Multidrug-Resistant Pseudomonas aeruginosa
Authors: Abd El-Aziz, A. M., Elgaml, A., Ali, Y. M.
Journal: J Infect Dis (2019): 1439-1447
Mechanics and Dynamics of Bacterial Cell Lysis
Authors: Wong, F., Amir, A.
Journal: Biophys J (2019): 2378-2389
Application of lysis system in bacterial vector vaccines
Authors: Tang, Y., Liu, Q., Li, P., Luo, H., Kong, Q.
Journal: Sheng Wu Gong Cheng Xue Bao (2019): 375-388
Quantifying bacterial cell lysis using GFP based fluorimetric assay
Authors: Sharma, M., Tyagi, J. L., Poluri, K. M.
Journal: Int J Biol Macromol (2019): 881-889
Viral Regulation on Bacterial Community Impacted by Lysis-Lysogeny Switch: A Microcosm Experiment in Eutrophic Coastal Waters
Authors: Chen, X., Ma, R., Yang, Y., Jiao, N., Zhang, R.
Journal: Front Microbiol (2019): 1763
Phage single-gene lysis: Finding the weak spot in the bacterial cell wall
Authors: Chamakura, K., Young, R.
Journal: J Biol Chem (2019): 3350-3358
FRET probe-based antibacterial susceptibility testing (F-AST) by detection of bacterial nucleases released by antibiotic-induced lysis
Authors: Park, K., Jeong, J., Yi, S. Y., Lee, W. S., Shin, Y. B.
Journal: Biosens Bioelectron (2019): 225-229
Use of a modified bacterial ghost lysis system for the construction of an inactivated avian pathogenic Escherichia coli vaccine candidate
Authors: Hu, J., Zuo, J., Chen, Z., Fu, L., Lv, X., Hu, S., Shi, X., Jing, Y., Wang, Y., Wang, Z., Mi, R., Huang, Y., Liu, D., Qi, K., Han, X.
Journal: Vet Microbiol (2019): 48-58
Dynamic bacterial community changes in the autothermal thermophilic aerobic digestion process with cell lysis activities, shaking and temperature increase
Authors: Cheng, H., Asakura, Y., K and a, K., Fukui, R., Kawano, Y., Okugawa, Y., Tashiro, Y., Sakai, K.
Journal: J Biosci Bioeng (2018): 196-204
An improved in-house lysis-filtration protocol for bacterial identification from positive blood culture bottles with high identification rates by MALDI-TOF MS
Authors: Tsuchida, S., Murata, S., Miyabe, A., Satoh, M., Takiwaki, M., Matsushita, K., Nomura, F.
Journal: J Microbiol Methods (2018): 40-45