logo
Products
Technologies
Applications
Services
Resources
Selection Guides
About
ProLite™ Orange Protein Gel Stain
5000X
Prolite™ Orange is an alternative protein stain that can be used to replace SYPRO Orange Protein Gel Stain (SYPRO is the trademark of ThermoFisher). Prolite™ Orange is a sensitive, ready-to-use fluorescent stain for total protein detection in 1D gels. The sensitivity of Prolite™ Orange is as good as or better than traditional silver staining techniques. Stained proteins can be viewed with a standard UV or blue-light transilluminator or imaging equipment containing the appropriate filters or lasers. Fluorescent stains are rapid, and highly sensitive for detecting total protein in protein electrophoresis gels and membranes. The Prolite™ Orange fluorescent stain can be used for total protein quantitation and can be viewed using a standard UV or blue-light transilluminator or with imaging instruments equipped with appropriate light sources.
Three-fold dilution series of BSA standards were separated on a NuPAGE&reg; 4&ndash;12% Bis-Tris gel and stained with A) ProLite&trade; Orange Protein Gel Stain or B)&nbsp;Coomassie brilliant blue (CBB) according to standard protocols. The ProLite&trade;&nbsp;Orange stained gels were photographed using a SYPRO Orange filter. The CBB-stained gels were photographed using transmitted white light&nbsp;without an optical filter.<br />Lane 1: 15ug, Lane 7: ~20ng, Lane 10: ~0.8 ng BSA.
Three-fold dilution series of BSA standards were separated on a NuPAGE&reg; 4&ndash;12% Bis-Tris gel and stained with A) ProLite&trade; Orange Protein Gel Stain or B)&nbsp;Coomassie brilliant blue (CBB) according to standard protocols. The ProLite&trade;&nbsp;Orange stained gels were photographed using a SYPRO Orange filter. The CBB-stained gels were photographed using transmitted white light&nbsp;without an optical filter.<br />Lane 1: 15ug, Lane 7: ~20ng, Lane 10: ~0.8 ng BSA.
protocol
Protocol
COA
CatalogSize
Price
Quantity
18000100 ul
Price
180011 ml
Price
 
Physical properties
Molecular weight486.72
SolventWater
Spectral properties
Excitation (nm)484
Emission (nm)586
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Contact us
Telephone
Fax
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Request quotationRequest
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Page updated on October 8, 2025