ProLite™ FAST Blue Protein Gel Stain 200 Gels

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Overview SDS Protocol

Platform

Gel Imager

Excitation	Coomassie Blue Filter Set
Emission	Coomassie Blue Filter Set

Components

Example protocol

PREPARATION OF STOCK SOLUTIONS

Prepare a stock solution by adding 500 µL of DMSO into the vial of ProLite™ FAST Blue Protein Gel Stain (Component A).

Note: Any unused stock solution should be divided into single-use aliquots and stored at ≤-20 °C for two weeks. Avoid repeated freeze-thaw cycles and minimize light exposure.

Note: The 500 µL stock solution can effectively stain up to 200 gels, with each gel containing 10 wells.

PREPARATION OF WORKING SOLUTION

Prepare a working solution by adding 50 µL of the ProLite™ FAST Blue Protein Gel Stain stock solution to 1 mL of Staining buffer (Component B).

Note: Any unused working solution should be divided into single-use aliquots and stored at ≤-20 °C for two weeks. Avoid repeated freeze-thaw cycles and minimize light exposure.

SAMPLE EXPERIMENTAL PROTOCOL

Add 0.25 µL of the ProLite™ FAST Blue Protein Gel Stain stock solution to 10 µL of the protein lysate.

Note: The dye volume can be adjusted based on the total volume and protein concentration used in the lysate.
Incubate the mixture at room temperature for 15 to 60 minutes, protected from light.
Add the loading dye to the protein lysate as per the manufacturer's guidelines.
Load the samples onto the gel and run.
Following the completion of the gel run, the samples should be visibly present within the gel.

Images

Figure 1. Bovine serum albumin (BSA) was stained with ProLite™ FAST Blue Protein Gel Stain (Cat No. 18002) for 60, 30, 15, and 5 minutes, and then loaded on a 4-12% Bis-Tris gel. A control sample was also included in the experiment, where no dye was added. The image was captured using a Coomassie Blue filter set.