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PageTell™ Prestained 10 to 250 kDa Protein Ladder

PageTell™ Prestained 10 to 250 kDa Protein Ladder
PageTell™ Prestained 10 to 250 kDa Protein Ladder
PageTell™ Prestained 10 to 250 kDa Protein Ladder
PageTell™ Prestained 10 to 250 kDa Protein Ladder was run on different percentages of Tris-Glycine gels.
PageTell™ Prestained 10 to 250 kDa Protein Ladder was run on diffrent percentages of Bis-Tris gels.
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Physical properties
Molecular weightN/A
SolventWater
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
N/A
PageTell™ Prestained 10 to 250 kDa Protein Ladder is a ready-to-use three-color protein standard with 13 pre-stained proteins covering a wide range of molecular weights from 10 to 250 kDa in Tris-Glycine buffer. Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa, respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The ready to use format helps the users to use it directly without the need to heat, reduce or add a sample buffer prior to use. It is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (nitrocellulose, PVDF, or nylon) and for approximating the size of proteins.

Example protocol


SAMPLE EXPERIMENTAL PROTOCOL

PageTell™ Prestained 10 to 250 kDa Protein Ladder is pre-formulated and ready to use. 5 µL per well is recommended to use for each gel.

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References


View all 15 references: Citation Explorer
A Single-Use, In Vitro Biosensor for the Detection of T-Tau Protein, A Biomarker of Neuro-Degenerative Disorders, in PBS and Human Serum Using Differential Pulse Voltammetry (DPV).
Authors: Dai, Yifan and Molazemhosseini, Alireza and Liu, Chung Chiun
Journal: Biosensors (2017)
Identification of pili on the surface of Finegoldia magna--a gram-positive anaerobic cocci.
Authors: Murphy, Elizabeth C and Janulczyk, Robert and Karlsson, Christofer and Mörgelin, Matthias and Frick, Inga-Maria
Journal: Anaerobe (2014): 40-9
A rapid standardized quantitative microfluidic system approach for evaluating human tear proteins.
Authors: Versura, Piera and Bavelloni, Alberto and Blalock, William and Fresina, Michela and Campos, Emilio C
Journal: Molecular vision (2012): 2526-37
A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis.
Authors: Kao, Chien-Han and Cheng, Chiu-Min and Chuang, Kuo-Hsiang and Chuang, Chih-Hung and Tzou, Shey-Cherng and Cheng, Ta-Chun and Hsieh, Yuan-Chin and Liao, Kuang-Wen and Wang, Yun-Ming and Chang, Long-Sen and Roffler, Steve R and Chen, Fang Ming and Cheng, Tian-Lu
Journal: Analytical biochemistry (2012): 1-3
[Characterization of Toxoplasma gondii proteins from various strains].
Authors: Yaman, Kerem and Akarsu, Gülay Aral and Güngör, Çiğdem and Ataoğlu, Haluk
Journal: Turkiye parazitolojii dergisi (2011): 133-6
Quantitative enzyme activity determination with zeptomole sensitivity by microfluidic gradient-gel zymography.
Authors: Hughes, Alex J and Herr, Amy E
Journal: Analytical chemistry (2010): 3803-11
Structural characterization of glycopeptides by N-terminal protein ladder sequencing.
Authors: Suzuki, Yusuke and Suzuki, Minoru and Nakahara, Yoshiaki and Ito, Yukishige and Ito, Emi and Goto, Naoko and Miseki, Kozo and Iida, Junko and Suzuki, Akemi
Journal: Analytical chemistry (2006): 2239-43
Climbing the protein ladder.
Authors: Dovichi, Norman J
Journal: Nature biotechnology (2004): 1242-3
Methods for on-chip protein analysis.
Authors: Caputo, Emilia and Moharram, Ramy and Martin, Brian M
Journal: Analytical biochemistry (2003): 116-24
A sodium dodecyl sulfate-polyacrylamide gel electrophoresis protein ladder made of disulfide-bridged proteins.
Authors: Doucet, A and Beauregard, M
Journal: Analytical biochemistry (2001): 296-7