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ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

Chemical structure for ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
Chemical structure for ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
Ordering information
Price ()
Catalog Number11001
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure
UNSPSC12171501
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OverviewpdfSDSpdfProtocol


CAS
30931-67-0
Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product. This chromogenic reaction is widely used for quantify HRP in ELISA assays. The oxidized ABTS product has the absorption maximum of 420 nm that can easily be followed with a spectrophotometer. ReadiUse™ ABTS Solution is optimized for ELISA assays that use HRP or HRP-labeled conjugates and hydrogen peroxide in microwell plates or test tubes. Our ABTS solution allows HRP reaction done in a single addition. The assay solution changes its color to light green upon its reaction with HRP or HRP conjugates in the presence of hydrogen peroxide.

Platform


Absorbance microplate reader

Recommended plateClear bottom

Example protocol


AT A GLANCE

Important      Warm ReadiUse™ ABTS Solution to room temperature before use. The reagent is to be used as supplied, no dilution is required.

SAMPLE EXPERIMENTAL PROTOCOL

  1. Wash the assay plate following the incubation of HRP-labeled reagent.
  2. Add 100 µL of ReadiUse™ ABTS Solution into each well.
  3. Incubate the plate at room temperature for 15 – 30 min.
    Note     The incubation time varies depending on the assay conditions.
  4. Measure the absorbance signal at 415 ± 10 nm (maximum at 420 nm) with an ELISA microplate reader.
    Note     If desired, the reaction can be stopped by adding an equal volume of 1% SDS or 0.01% sodium azide into 0.1 M citric acid. Stopped reaction should be read within 30 minutes. 

References


View all 28 references: Citation Explorer
Plant extracts with anti-inflammatory properties--a new approach for characterization of their bioactive compounds and establishment of structure-antioxidant activity relationships
Authors: Amaral S, Mira L, Nogueira JM, da Silva AP, Helena Florencio M.
Journal: Bioorg Med Chem (2009): 1876
A three-enzyme cascade reaction through positional assembly of enzymes in a polymersome nanoreactor
Authors: van Dongen SF, Nallani M, Cornelissen JJ, Nolte RJ, van Hest JC.
Journal: Chemistry (2009): 1107
Degradation of glyphosate and other pesticides by ligninolytic enzymes
Authors: Pizzul L, Castillo MD, Stenstrom J.
Journal: Biodegradation. (2009)
Self-assembly of enzymes on DNA scaffolds: en route to biocatalytic cascades and the synthesis of metallic nanowires
Authors: Wilner OI, Shimron S, Weizmann Y, Wang ZG, Willner I.
Journal: Nano Lett (2009): 2040
Supramolecular cocaine-aptamer complexes activate biocatalytic cascades
Authors: Freeman R, Sharon E, Tel-Vered R, Willner I.
Journal: J Am Chem Soc (2009): 5028
Cooperative multicomponent self-assembly of nucleic acid structures for the activation of DNAzyme cascades: a paradigm for DNA sensors and aptasensors
Authors: Elbaz J, Moshe M, Shlyahovsky B, Willner I.
Journal: Chemistry (2009): 3411
Full-field photonic biosensors based on tunable bio-doped sol-gel glasses
Authors: Llobera A, Cadarso VJ, Darder M, Dominguez C, Fern and ez-Sanchez C., undefined
Journal: Lab Chip (2008): 1185
Simultaneous total antioxidant capacity assay of lipophilic and hydrophilic antioxidants in the same acetone-water solution containing 2% methyl-beta-cyclodextrin using the cupric reducing antioxidant capacity (CUPRAC) method
Authors: Ozyurek M, Bektasoglu B, Guclu K, Gungor N, Apak R.
Journal: Anal Chim Acta (2008): 28
Effects of mutations in the helix G region of horseradish peroxidase
Authors: Ryan BJ, O'Fagain C.
Journal: Biochimie (2008): 1414
Glutamic acid-141: a heme 'bodyguard' in anionic tobacco peroxidase
Authors: Hushpulian DM, Poloznikov AA, Savitski PA, Rozhkova AM, Chubar TA, Fechina VA, Orlova MA, Tishkov VI, Gazaryan IG, Lagrimini LM.
Journal: Biol Chem (2007): 373