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ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

Chemical structure for ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
Chemical structure for ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
Ordering information
Price ()
Catalog Number11001
Unit Size
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Additional ordering information
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageRefrigerated (2-8 °C); Minimize light exposure


Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product. This chromogenic reaction is widely used for quantify HRP in ELISA assays. The oxidized ABTS product has the absorption maximum of 420 nm that can easily be followed with a spectrophotometer. ReadiUse™ ABTS Solution is optimized for ELISA assays that use HRP or HRP-labeled conjugates and hydrogen peroxide in microwell plates or test tubes. Our ABTS solution allows HRP reaction done in a single addition. The assay solution changes its color to light green upon its reaction with HRP or HRP conjugates in the presence of hydrogen peroxide.


Absorbance microplate reader

Recommended plateClear bottom

Example protocol


Important      Warm ReadiUse™ ABTS Solution to room temperature before use. The reagent is to be used as supplied, no dilution is required.


  1. Wash the assay plate following the incubation of HRP-labeled reagent.
  2. Add 100 µL of ReadiUse™ ABTS Solution into each well.
  3. Incubate the plate at room temperature for 15 – 30 min.
    Note     The incubation time varies depending on the assay conditions.
  4. Measure the absorbance signal at 415 ± 10 nm (maximum at 420 nm) with an ELISA microplate reader.
    Note     If desired, the reaction can be stopped by adding an equal volume of 1% SDS or 0.01% sodium azide into 0.1 M citric acid. Stopped reaction should be read within 30 minutes. 


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Simultaneous total antioxidant capacity assay of lipophilic and hydrophilic antioxidants in the same acetone-water solution containing 2% methyl-beta-cyclodextrin using the cupric reducing antioxidant capacity (CUPRAC) method
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Journal: Anal Chim Acta (2008): 28
Effects of mutations in the helix G region of horseradish peroxidase
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Glutamic acid-141: a heme 'bodyguard' in anionic tobacco peroxidase
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