Name: ReadiUse™ Preactivated HRP maleimide
Brand: AAT Bioquest
SKU: 11026
Price: 118 USD
Availability: InStock

ReadiUse™ Preactivated HRP maleimide

ReadiUse™ Preactivated HRP Maleimide is a thiol-reactive horseradish peroxidase reagent for conjugating antibodies and other biomolecules for enzyme-based detection assays.

Maleimide Chemistry: Enables covalent conjugation to thiol groups.
Ready-to-Use: Preactivated HRP simplifies antibody conjugation.
Rapid Conjugation: Completes labeling in 1–2 hours at pH 6–7.
Minimal Purification: Conjugates can often be used without further purification.
Applications: Suitable for HRP-based antibody labeling and ELISA assays.

<p>ReadiUse™ Preactivated HRP maleimide can be used to label thiol-containing proteins in the manner shown. The reaction is a  simple 1-2 hours mixing and in most cases does not require further purification before use.</p>

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
11026	300 ug	Price

Overview

ReadiUse™ Preactivated maleimide is a thiol-reactive HRP derivative. It can be used to readily label proteins (such as antibodies) or other biologicals molecules that have a thiol group. ReadiUse™ Preactivated HRP maleimide is robust and easy to use. It can be used to label antibodies with a simple mixing, i.e. the slightly acidic or neutral solution of an antibody (pH 6-7) can be directly mixed with the HRP maleimide, and the reaction mixture is shaken for 1-2 hours. In most cases, the resulted solution can be directly used for ELISA assays without further purification.

Citations

View all 10 citations: Citation Explorer

Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase

Authors:

Milton, Amber

Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA

Authors:

Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze

Journal:

Analytical Chemistry (2017)

Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury

Authors:

Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T

Journal:

Radiology (2016): 152222

Myeloperoxidase--Hepatocyte--Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis

Authors:

Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W

Journal:

Antioxidants & redox signaling (2015): 1255--1269

Myeloperoxidase Nuclear Imaging for Epileptogenesis

Authors:

Zhang, Yinian and Seeburg, Daniel P and Pulli, Benjamin and Wojtkiewicz, Gregory R and Bure, Lionel and Atkinson, Wendy and Schob, Stefan and Iwamoto, Yoshiko and Ali, Muhammad and Zhang, Wei and others, undefined

Journal:

Radiology (2015): 822--830

References

View all 61 references: Citation Explorer

K+-independent actions of diazoxide question the role of inner membrane KATP channels in mitochondrial cytoprotective signaling

Authors:

Drose S, Br and t U, Hanley PJ.

Journal:

J Biol Chem (2006): 23733

Real-time assessment of spatial and temporal coupled catalysis within polyelectrolyte microcapsules containing coimmobilized glucose oxidase and peroxidase

Authors:

Stein EW, Volodkin DV, McShane MJ, Sukhorukov GB.

Journal:

Biomacromolecules (2006): 710

O2 Delivery and Redox State are Determinants of Compartment-specific Reactive Oxygen Species in Myocardial Reperfusion

Authors:

Stoner JD, Clanton TL, Aune SE, Angelos MG.

Journal:

Am J Physiol Heart Circ Physiol. (2006)

Fibrillar beta-amyloid peptide Abeta1-40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: a cell culture study

Authors:

Jekabsone A, M and er PK, Tickler A, Sharpe M, Brown GC.

Journal:

J Neuroinflammation (2006): 24

Biochemical activity of reactive oxygen species scavengers do not predict retinal ganglion cell survival

Authors:

Schlieve CR, Lieven CJ, Levin LA.

Journal:

Invest Ophthalmol Vis Sci (2006): 3878

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C)

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Page updated on October 28, 2026