ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

ReadiUse™ ABTS Substrate Solution

Optimized for ELISA Assays with HRP Conjugates

ReadiUse™ ABTS Substrate Solution is a ready-to-use chromogenic substrate for detecting horseradish peroxidase (HRP) activity in ELISA and other enzyme-based assays.

Colorimetric Detection: Produces a blue-green product measurable at 420 nm.
HRP-Based Chemistry: ABTS oxidation generates a colorimetric signal in the presence of HRP and hydrogen peroxide.
Ready-to-Use: Preformulated solution requires no dilution.
Simple Workflow: Single-addition assay with a 15–30 minute incubation.
Assay Compatibility: Suitable for HRP-based ELISAs in microplates or test tubes.

Chemical structure for ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*

Protocol

Catalog	Size	Price	Quantity
11001	1 L	Price
11013	100 mL	Price

Overview

Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product. This chromogenic reaction is widely used for quantify HRP in ELISA assays. The oxidized ABTS product has the absorption maximum of 420 nm that can easily be followed with a spectrophotometer. ReadiUse™ ABTS Solution is optimized for ELISA assays that use HRP or HRP-labeled conjugates and hydrogen peroxide in microwell plates or test tubes. Our ABTS solution allows HRP reaction done in a single addition. The assay solution changes its color to light green upon its reaction with HRP or HRP conjugates in the presence of hydrogen peroxide.

Citations

View all 3 citations: Citation Explorer

A skin colonizer disrupts inflammatory and humoral immune defenses in hidradenitis suppurativa

Authors:

Agbogan, Viviane A and Bugault, Florence and Guenin-Mac{\'e}, Laure and Gribonika, Inta and Planchais, Cyril and Morel, Jean-David and Delaleu, J{\'e}r{\'e}mie and P{\'e}rez-Chaparro, P Juliana and Atlan, Michael and Delage, Ma{\"\i}a and others,

Journal:

EMBO Molecular Medicine (2026): 1--27

Broad Sarbecovirus Neutralization by Combined Memory B-cell Antibodies to Ancestral SARS-CoV-2

Authors:

Planchais, Cyril and Fern{\'a}ndez, Ignacio and Chalopin, Benjamin and Bruel, Timoth{\'e}e and Rosenbaum, Pierre and Beretta, Maxime and Dimitrov, Jordan D and Conquet, Laurine and Donati, Flora and Prot, Matthieu and others,

Journal:

iScience (2024)

Anti-V1/V3-glycan broadly HIV-1 neutralizing antibodies in a post-treatment controller

Authors:

Molinos-Albert, Luis M and Baquero, Eduard and Bouvin-Pley, M{\'e}lanie and Lorin, Val{\'e}rie and Charre, Caroline and Planchais, Cyril and Dimitrov, Jordan D and Monceaux, Val{\'e}rie and Vos, Matthijn and Hocqueloux, Laurent and others,

Journal:

Cell Host \& Microbe

References

View all 28 references: Citation Explorer

Plant extracts with anti-inflammatory properties--a new approach for characterization of their bioactive compounds and establishment of structure-antioxidant activity relationships

Authors:

Amaral S, Mira L, Nogueira JM, da Silva AP, Helena Florencio M.

Journal:

Bioorg Med Chem (2009): 1876

A three-enzyme cascade reaction through positional assembly of enzymes in a polymersome nanoreactor

Authors:

van Dongen SF, Nallani M, Cornelissen JJ, Nolte RJ, van Hest JC.

Journal:

Chemistry (2009): 1107

Degradation of glyphosate and other pesticides by ligninolytic enzymes

Authors:

Pizzul L, Castillo MD, Stenstrom J.

Journal:

Biodegradation. (2009)

Self-assembly of enzymes on DNA scaffolds: en route to biocatalytic cascades and the synthesis of metallic nanowires

Authors:

Wilner OI, Shimron S, Weizmann Y, Wang ZG, Willner I.

Journal:

Nano Lett (2009): 2040

Supramolecular cocaine-aptamer complexes activate biocatalytic cascades

Authors:

Freeman R, Sharon E, Tel-Vered R, Willner I.

Journal:

J Am Chem Soc (2009): 5028

Physical properties

Molecular weight

N/A

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure
CAS	30931-67-0

Instrument settings

Absorbance microplate reader
Absorbance	420 nm
Recommended plate	Clear bottom

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Page updated on October 24, 2026