ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Refrigerated (2-8 °C); Minimize light exposure |
| UNSPSC | 12171501 |
Related products
| Overview |  SDSProtocol |
See also: Antibodies and Proteomics, Antibody and Protein Labeling, Bioconjugation, Protein to Protein Conjugation, Biotin and Streptavidin, Enzyme-Linked Immunosorbent Assay (ELISA), Enzymes, Kinases, Horseradish Peroxidase (HRP) and Poly-HRP, Immunohistochemistry (IHC), Oxidases, Secondary Antibodies, Serology Assays for Antibody Detection, Western Blotting Assays
CAS 30931-67-0 |
Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product. This chromogenic reaction is widely used for quantify HRP in ELISA assays. The oxidized ABTS product has the absorption maximum of 420 nm that can easily be followed with a spectrophotometer. ReadiUse™ ABTS Solution is optimized for ELISA assays that use HRP or HRP-labeled conjugates and hydrogen peroxide in microwell plates or test tubes. Our ABTS solution allows HRP reaction done in a single addition. The assay solution changes its color to light green upon its reaction with HRP or HRP conjugates in the presence of hydrogen peroxide.
Platform
Absorbance microplate reader
| Absorbance | 420 nm |
| Recommended plate | Clear bottom |
Example protocol
AT A GLANCE
Important Warm ReadiUse™ ABTS Solution to room temperature before use. The reagent is to be used as supplied, no dilution is required.
SAMPLE EXPERIMENTAL PROTOCOL
- Wash the assay plate following the incubation of HRP-labeled reagent.
- Add 100 µL of ReadiUse™ ABTS Solution into each well.
- Incubate the plate at room temperature for 15 – 30 min.
Note The incubation time varies depending on the assay conditions. - Measure the absorbance signal at 415 ± 10 nm (maximum at 420 nm) with an ELISA microplate reader.
Note If desired, the reaction can be stopped by adding an equal volume of 1% SDS or 0.01% sodium azide into 0.1 M citric acid. Stopped reaction should be read within 30 minutes.
Images
Figure 1. Chemical structure for ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
References
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