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ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
Horseradish peroxidase (HRP) and HRP conjugates facilitate the ABTS oxidation in the presence of hydrogen peroxide, turning ABTS into its blue-green oxidized product. This chromogenic reaction is widely used for quantify HRP in ELISA assays. The oxidized ABTS product has the absorption maximum of 420 nm that can easily be followed with a spectrophotometer. ReadiUse™ ABTS Solution is optimized for ELISA assays that use HRP or HRP-labeled conjugates and hydrogen peroxide in microwell plates or test tubes. Our ABTS solution allows HRP reaction done in a single addition. The assay solution changes its color to light green upon its reaction with HRP or HRP conjugates in the presence of hydrogen peroxide.
Example protocol
AT A GLANCE
Important Warm ReadiUse™ ABTS Solution to room temperature before use. The reagent is to be used as supplied, no dilution is required.
SAMPLE EXPERIMENTAL PROTOCOL
- Wash the assay plate following the incubation of HRP-labeled reagent.
- Add 100 µL of ReadiUse™ ABTS Solution into each well.
- Incubate the plate at room temperature for 15 – 30 min.
Note The incubation time varies depending on the assay conditions. - Measure the absorbance signal at 415 ± 10 nm (maximum at 420 nm) with an ELISA microplate reader.
Note If desired, the reaction can be stopped by adding an equal volume of 1% SDS or 0.01% sodium azide into 0.1 M citric acid. Stopped reaction should be read within 30 minutes.
Citations
View all 2 citations: Citation Explorer
Broad Sarbecovirus Neutralization by Combined Memory B-cell Antibodies to Ancestral SARS-CoV-2
Authors: Planchais, Cyril and Fern{\'a}ndez, Ignacio and Chalopin, Benjamin and Bruel, Timoth{\'e}e and Rosenbaum, Pierre and Beretta, Maxime and Dimitrov, Jordan D and Conquet, Laurine and Donati, Flora and Prot, Matthieu and others,
Journal: iScience (2024)
Authors: Planchais, Cyril and Fern{\'a}ndez, Ignacio and Chalopin, Benjamin and Bruel, Timoth{\'e}e and Rosenbaum, Pierre and Beretta, Maxime and Dimitrov, Jordan D and Conquet, Laurine and Donati, Flora and Prot, Matthieu and others,
Journal: iScience (2024)
Anti-V1/V3-glycan broadly HIV-1 neutralizing antibodies in a post-treatment controller
Authors: Molinos-Albert, Luis M and Baquero, Eduard and Bouvin-Pley, M{\'e}lanie and Lorin, Val{\'e}rie and Charre, Caroline and Planchais, Cyril and Dimitrov, Jordan D and Monceaux, Val{\'e}rie and Vos, Matthijn and Hocqueloux, Laurent and others,
Journal: Cell Host \& Microbe
Authors: Molinos-Albert, Luis M and Baquero, Eduard and Bouvin-Pley, M{\'e}lanie and Lorin, Val{\'e}rie and Charre, Caroline and Planchais, Cyril and Dimitrov, Jordan D and Monceaux, Val{\'e}rie and Vos, Matthijn and Hocqueloux, Laurent and others,
Journal: Cell Host \& Microbe
References
View all 28 references: Citation Explorer
Plant extracts with anti-inflammatory properties--a new approach for characterization of their bioactive compounds and establishment of structure-antioxidant activity relationships
Authors: Amaral S, Mira L, Nogueira JM, da Silva AP, Helena Florencio M.
Journal: Bioorg Med Chem (2009): 1876
Authors: Amaral S, Mira L, Nogueira JM, da Silva AP, Helena Florencio M.
Journal: Bioorg Med Chem (2009): 1876
A three-enzyme cascade reaction through positional assembly of enzymes in a polymersome nanoreactor
Authors: van Dongen SF, Nallani M, Cornelissen JJ, Nolte RJ, van Hest JC.
Journal: Chemistry (2009): 1107
Authors: van Dongen SF, Nallani M, Cornelissen JJ, Nolte RJ, van Hest JC.
Journal: Chemistry (2009): 1107
Degradation of glyphosate and other pesticides by ligninolytic enzymes
Authors: Pizzul L, Castillo MD, Stenstrom J.
Journal: Biodegradation. (2009)
Authors: Pizzul L, Castillo MD, Stenstrom J.
Journal: Biodegradation. (2009)
Self-assembly of enzymes on DNA scaffolds: en route to biocatalytic cascades and the synthesis of metallic nanowires
Authors: Wilner OI, Shimron S, Weizmann Y, Wang ZG, Willner I.
Journal: Nano Lett (2009): 2040
Authors: Wilner OI, Shimron S, Weizmann Y, Wang ZG, Willner I.
Journal: Nano Lett (2009): 2040
Supramolecular cocaine-aptamer complexes activate biocatalytic cascades
Authors: Freeman R, Sharon E, Tel-Vered R, Willner I.
Journal: J Am Chem Soc (2009): 5028
Authors: Freeman R, Sharon E, Tel-Vered R, Willner I.
Journal: J Am Chem Soc (2009): 5028
Page updated on May 19, 2025
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