ReadiUse™ DRAQ5 Staining Solution 5 mM in Water

Live Cell Cycle analysis using DRAQ5. Jurkat cells were stained with 10uM DRAQ5 in growth medium for 15min, and analyzed with flow cytometer APC channel.

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	485.41
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	22000¹
Excitation (nm)	601
Emission (nm)	699

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Refrigerated (2-8 °C); Minimize light exposure; Desiccated
UNSPSC	41116134

Overview SDS Protocol

CAS

252903-95-0

Molecular weight

485.41

Extinction coefficient (cm ^-1 M ^-1)

22000¹

Excitation (nm)

601

Emission (nm)

699

DRAQ5™ is the trade name of 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione (DRAQ-5™ is the trade mark of Biostatus Ltd). It is a far-red DNA stain for live or fixed cells, and it can be readily used for multiplexing with other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins. It is lipophilic and membrane permeable dye for live-cell and fixed-cell staining and quantitation of DNA content. It is also ideal for use alongside other fluorophore reporters for fluorescence microscopy or high-content screening (HCS). Due to its far-red excitation and emission, DRAQ-5 Stain can be multiplexed with many other fluorophores. It is commonly chosen to counterstain nuclei and measure DNA content in cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins. DRAQ-5 is compatible with many existing protocols across a wide range of instrumentation platforms. AAT Bioquest offers this ready to use solution to facilitate your fluorescence imaging and flow cytometric tests.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of ReadiUse™ DRAQ5 Staining Solution *5 mM in Water* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	206.011 µL	1.03 mL	2.06 mL	10.301 mL	20.601 mL
5 mM	41.202 µL	206.011 µL	412.023 µL	2.06 mL	4.12 mL
10 mM	20.601 µL	103.006 µL	206.011 µL	1.03 mL	2.06 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	22000¹
Excitation (nm)	601
Emission (nm)	699

Images

Figure 1. Live Cell Cycle analysis using DRAQ5. Jurkat cells were stained with 10uM DRAQ5 in growth medium for 15min, and analyzed with flow cytometer APC channel.

Figure 2. Chemical structure for ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*.

Citations

View all 7 citations: Citation Explorer

Preparing Planarian Cells for High-content Fluorescence Microscopy Using RNA in situ Hybridization and Immunocytochemistry
Authors: Grohme, Markus and Frank, Olga and Rink, Jochen
Journal: (2021)

One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124

DRAQ5 and Eosin ('D&E') as an Analog to Hematoxylin and Eosin for Rapid Fluorescence Histology of Fresh Tissues
Authors: Elfer, K. N., Sholl, A. B., Wang, M., Tulman, D. B., M and ava, S. H., Lee, B. R., Brown, J. Q.
Journal: PLoS One (2016): e0165530

Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1

Short exposure to the DNA intercalator DRAQ5 dislocates the transcription machinery and induces cell death
Authors: Richard, E., Causse, S., Spriet, C., Fourre, N., Trinel, D., Darzacq, X., V and enbunder, B., Heliot, L.
Journal: Photochem Photobiol (2011): 256-61

The use of DRAQ5 to monitor intracellular DNA in Escherichia coli by flow cytometry
Authors: Silva, F., Lourenco, O., Pina-Vaz, C., Rodrigues, A. G., Queiroz, J. A., Domingues, F. C.
Journal: J Fluoresc (2010): 907-14

Influence of the live cell DNA marker DRAQ5 on chromatin-associated processes
Authors: Mari, P. O., Verbiest, V., Sabbioneda, S., Gourdin, A. M., Wijgers, N., Dinant, C., Lehmann, A. R., Vermeulen, W., Giglia-Mari, G.
Journal: DNA Repair (Amst) (2010): 848-55

References

View all 1 references: Citation Explorer

A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry
Authors: Smith PJ, Wiltshire M, Davies S, Patterson LH, Hoy T.
Journal: J Immunol Methods (1999): 131