Name: ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*
Brand: AAT Bioquest
SKU: 17558
Price: 178 USD
Availability: InStock

ReadiUse™ DRAQ5 Staining Solution

5 mM in Water

ReadiUse™ DRAQ5 Staining Solution is a membrane-permeable, far-red fluorescent DNA stain for nuclear labeling and DNA content analysis in live and fixed cells.

Fluorescence Detection: Excitation at 601 nm and emission at 699 nm.
Cell Permeability: Enables nuclear staining in live and fixed cells.
Multiplex Compatibility: Far-red fluorescence supports co-staining with GFP and other fluorophores.
Applications: Suitable for flow cytometry, fluorescence microscopy, and high-content screening.

Live Cell Cycle analysis using DRAQ5. Jurkat cells were stained with 10uM DRAQ5 in growth medium for 15min, and analyzed with flow cytometer APC channel.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
17558	50 uL	Price

Overview

DRAQ5™ is the trade name of 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione (DRAQ-5™ is the trade mark of Biostatus Ltd). It is a far-red DNA stain for live or fixed cells, and it can be readily used for multiplexing with other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins. It is lipophilic and membrane permeable dye for live-cell and fixed-cell staining and quantitation of DNA content. It is also ideal for use alongside other fluorophore reporters for fluorescence microscopy or high-content screening (HCS). Due to its far-red excitation and emission, DRAQ-5 Stain can be multiplexed with many other fluorophores. It is commonly chosen to counterstain nuclei and measure DNA content in cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins. DRAQ-5 is compatible with many existing protocols across a wide range of instrumentation platforms. AAT Bioquest offers this ready to use solution to facilitate your fluorescence imaging and flow cytometric tests.

Citations

View all 8 citations: Citation Explorer

Cholesterol depletion suppresses thermal necrosis resistance by alleviating an increase in membrane fluidity

Authors:

Kanamori, Taisei and Yasuda, Shogo and Duan, Runjing and Ohashi, Mei and Amou, Mai and Hori, Kanato and Tsuda, Ryota and Fujimoto, Taiki and Higashi, Kenjirou and Xu, Wei and others,

Journal:

Scientific Reports (2025): 10133

Preparing Planarian Cells for High-content Fluorescence Microscopy Using RNA in situ Hybridization and Immunocytochemistry

Authors:

Grohme, Markus and Frank, Olga and Rink, Jochen

DRAQ5 and Eosin ('D&E') as an Analog to Hematoxylin and Eosin for Rapid Fluorescence Histology of Fresh Tissues

Authors:

Elfer, K. N., Sholl, A. B., Wang, M., Tulman, D. B., M and ava, S. H., Lee, B. R., Brown, J. Q.

Journal:

PLoS One (2016): e0165530

One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays

Authors:

Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei

Journal:

Journal of Biomolecular Screening (2016): 1112--1124

Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production

Authors:

Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe

Journal:

Microbial cell factories (2015): 1

Physical properties

Molecular weight	485.41
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	22000 ¹
Excitation (nm)	601
Emission (nm)	699

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure; Desiccated
CAS	252903-95-0

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Page updated on October 28, 2026