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ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*
DRAQ5™ is the trade name of 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione (DRAQ-5™ is the trade mark of Biostatus Ltd). It is a far-red DNA stain for live or fixed cells, and it can be readily used for multiplexing with other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins. It is lipophilic and membrane permeable dye for live-cell and fixed-cell staining and quantitation of DNA content. It is also ideal for use alongside other fluorophore reporters for fluorescence microscopy or high-content screening (HCS). Due to its far-red excitation and emission, DRAQ-5 Stain can be multiplexed with many other fluorophores. It is commonly chosen to counterstain nuclei and measure DNA content in cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins. DRAQ-5 is compatible with many existing protocols across a wide range of instrumentation platforms. AAT Bioquest offers this ready to use solution to facilitate your fluorescence imaging and flow cytometric tests.
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Citations
View all 8 citations: Citation Explorer
Cholesterol depletion suppresses thermal necrosis resistance by alleviating an increase in membrane fluidity
Authors: Kanamori, Taisei and Yasuda, Shogo and Duan, Runjing and Ohashi, Mei and Amou, Mai and Hori, Kanato and Tsuda, Ryota and Fujimoto, Taiki and Higashi, Kenjirou and Xu, Wei and others,
Journal: Scientific Reports (2025): 10133
Authors: Kanamori, Taisei and Yasuda, Shogo and Duan, Runjing and Ohashi, Mei and Amou, Mai and Hori, Kanato and Tsuda, Ryota and Fujimoto, Taiki and Higashi, Kenjirou and Xu, Wei and others,
Journal: Scientific Reports (2025): 10133
Preparing Planarian Cells for High-content Fluorescence Microscopy Using RNA in situ Hybridization and Immunocytochemistry
Authors: Grohme, Markus and Frank, Olga and Rink, Jochen
Journal: (2021)
Authors: Grohme, Markus and Frank, Olga and Rink, Jochen
Journal: (2021)
DRAQ5 and Eosin ('D&E') as an Analog to Hematoxylin and Eosin for Rapid Fluorescence Histology of Fresh Tissues
Authors: Elfer, K. N., Sholl, A. B., Wang, M., Tulman, D. B., M and ava, S. H., Lee, B. R., Brown, J. Q.
Journal: PLoS One (2016): e0165530
Authors: Elfer, K. N., Sholl, A. B., Wang, M., Tulman, D. B., M and ava, S. H., Lee, B. R., Brown, J. Q.
Journal: PLoS One (2016): e0165530
One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1
References
View all 1 references: Citation Explorer
A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry
Authors: Smith PJ, Wiltshire M, Davies S, Patterson LH, Hoy T.
Journal: J Immunol Methods (1999): 131
Authors: Smith PJ, Wiltshire M, Davies S, Patterson LH, Hoy T.
Journal: J Immunol Methods (1999): 131
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