AAT Bioquest

ReadiUse™ DRAQ5 Staining Solution *5 mM in Water*

Live Cell Cycle analysis using DRAQ5. Jurkat cells were stained with 10uM DRAQ5 in growth medium for 15min, and analyzed with flow cytometer APC channel.
Live Cell Cycle analysis using DRAQ5. Jurkat cells were stained with 10uM DRAQ5 in growth medium for 15min, and analyzed with flow cytometer APC channel.
Live Cell Cycle analysis using DRAQ5. Jurkat cells were stained with 10uM DRAQ5 in growth medium for 15min, and analyzed with flow cytometer APC channel.
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Physical properties
Molecular weight485.41
Spectral properties
Extinction coefficient (cm -1 M -1)220001
Excitation (nm)601
Emission (nm)699
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageRefrigerated (2-8 °C); Minimize light exposure; Desiccated
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Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
DRAQ5™ is the trade name of 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione (DRAQ-5™ is the trade mark of Biostatus Ltd). It is a far-red DNA stain for live or fixed cells, and it can be readily used for multiplexing with other fluorophores, such as cells expressing green fluorescent protein (GFP) fusion proteins. It is lipophilic and membrane permeable dye for live-cell and fixed-cell staining and quantitation of DNA content. It is also ideal for use alongside other fluorophore reporters for fluorescence microscopy or high-content screening (HCS). Due to its far-red excitation and emission, DRAQ-5 Stain can be multiplexed with many other fluorophores. It is commonly chosen to counterstain nuclei and measure DNA content in cell proliferation studies involving cells expressing green fluorescent protein (GFP) fusion proteins. DRAQ-5 is compatible with many existing protocols across a wide range of instrumentation platforms. AAT Bioquest offers this ready to use solution to facilitate your fluorescence imaging and flow cytometric tests.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of ReadiUse™ DRAQ5 Staining Solution *5 mM in Water* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM206.011 µL1.03 mL2.06 mL10.301 mL20.601 mL
5 mM41.202 µL206.011 µL412.023 µL2.06 mL4.12 mL
10 mM20.601 µL103.006 µL206.011 µL1.03 mL2.06 mL

Molarity calculator

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Spectral properties

Extinction coefficient (cm -1 M -1)220001
Excitation (nm)601
Emission (nm)699



View all 7 citations: Citation Explorer
DRAQ5 and Eosin ('D&E') as an Analog to Hematoxylin and Eosin for Rapid Fluorescence Histology of Fresh Tissues
Authors: Elfer, K. N., Sholl, A. B., Wang, M., Tulman, D. B., M and ava, S. H., Lee, B. R., Brown, J. Q.
Journal: PLoS One (2016): e0165530
One-Step Seeding of Neural Stem Cells with Vitronectin-Supplemented Medium for High-Throughput Screening Assays
Authors: Dai, Sheng and Li, Rong and Long, Yan and Titus, Steve and Zhao, Jinghua and Huang, Ruili and Xia, Menghang and Zheng, Wei
Journal: Journal of Biomolecular Screening (2016): 1112--1124
Genome shuffling of the nonconventional yeast Pichia anomala for improved sugar alcohol production
Authors: Zhang, Guoqiang and Lin, Yuping and Qi, Xianni and Wang, Lixian and He, Peng and Wang, Qinhong and Ma, Yanhe
Journal: Microbial cell factories (2015): 1
Short exposure to the DNA intercalator DRAQ5 dislocates the transcription machinery and induces cell death
Authors: Richard, E., Causse, S., Spriet, C., Fourre, N., Trinel, D., Darzacq, X., V and enbunder, B., Heliot, L.
Journal: Photochem Photobiol (2011): 256-61
Influence of the live cell DNA marker DRAQ5 on chromatin-associated processes
Authors: Mari, P. O., Verbiest, V., Sabbioneda, S., Gourdin, A. M., Wijgers, N., Dinant, C., Lehmann, A. R., Vermeulen, W., Giglia-Mari, G.
Journal: DNA Repair (Amst) (2010): 848-55
The use of DRAQ5 to monitor intracellular DNA in Escherichia coli by flow cytometry
Authors: Silva, F., Lourenco, O., Pina-Vaz, C., Rodrigues, A. G., Queiroz, J. A., Domingues, F. C.
Journal: J Fluoresc (2010): 907-14


View all 1 references: Citation Explorer
A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry
Authors: Smith PJ, Wiltshire M, Davies S, Patterson LH, Hoy T.
Journal: J Immunol Methods (1999): 131