ReadiUse™ Preactivated HRP NHS ester

ReadiUse™ Preactivated HRP NHS Ester is an amine-reactive horseradish peroxidase reagent for conjugating antibodies and other biomolecules for enzyme-based detection assays.

NHS Ester Chemistry: Enables covalent conjugation to primary amines.
Ready-to-Use: Preactivated HRP simplifies antibody conjugation.
Rapid Conjugation: Completes labeling in 1–2 hours at pH 8.5–9.0.
Minimal Purification: Conjugates can often be used without further purification.
Applications: Suitable for HRP-based antibody labeling and ELISA assays.

<p>Mechanism for conjugation of horseradish peroxidase (HRP) to target protein using activated NHS ester.</p>

Protocol

SDS

Catalog	Size	Price	Quantity
11025	300 ug	Price

Overview

ReadiUse™ Preactivated HRP NHS ester is the mono NHS ester of HRP. It can be used to readily label proteins (such as antibodies) or other biologicals molecules that have an amino group. ReadiUse™ Preactivated HRP NHS ester is robust and easy to use. It can be used to label antibodies with a simple mixing, i.e. the basic solution of an antibody (pH 8.5-9.0) can be directly mixed with the HRP NHS ester, and the reaction mixture is shaken for 1-2 hours. In most cases, the resulted solution can be directly used for ELISA assays without further purification. ReadiUse™ Preactivated HRP NHS ester provides the most robust method to perform the conjugation of antibodies with HRP. Any suitable amount of antibody (>1 mg/mL) can be readily conjugated to the preactivated HRP NHS ester without the need for purification.

Citations

View all 12 citations: Citation Explorer

Florfenicol binding to molecularly imprinted polymer nanoparticles in model and real samples

Authors:

Caro, Nelson and Bruna, Tamara and Guerreiro, Antonio and Alvarez-Tejos, Paola and Garret{\'o}n, Virginia and Piletsky, Sergey and Gonz{\'a}lez-Casanova, Jorge and Rojas-G{\'o}mez, Diana and Ehrenfeld, Nicole

Journal:

Nanomaterials (2020): 306

New method for immobilising diverse proteins onto cubic micro-protein polyhedrin crystals

Authors:

Yuasa, Haruna and Kotani, Eiji and Mori, Hajime and Takaki, Keiko

Journal:

Protein expression and purification (2019): 105531

Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase

Authors:

Milton, Amber

Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA

Authors:

Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze

Journal:

Analytical Chemistry (2017)

Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury

Authors:

Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T

Journal:

Radiology (2016): 152222

References

View all 61 references: Citation Explorer

Evaluation of assays for the measurement of bovine neutrophil reactive oxygen species

Authors:

Rinaldi M, Moroni P, Paape MJ, Bannerman DD.

Journal:

Vet Immunol Immunopathol. (2006)

Real-time assessment of spatial and temporal coupled catalysis within polyelectrolyte microcapsules containing coimmobilized glucose oxidase and peroxidase

Authors:

Stein EW, Volodkin DV, McShane MJ, Sukhorukov GB.

Journal:

Biomacromolecules (2006): 710

O2 Delivery and Redox State are Determinants of Compartment-specific Reactive Oxygen Species in Myocardial Reperfusion

Authors:

Stoner JD, Clanton TL, Aune SE, Angelos MG.

Journal:

Am J Physiol Heart Circ Physiol. (2006)

Fibrillar beta-amyloid peptide Abeta1-40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: a cell culture study

Authors:

Jekabsone A, M and er PK, Tickler A, Sharpe M, Brown GC.

Journal:

J Neuroinflammation (2006): 24

Biochemical activity of reactive oxygen species scavengers do not predict retinal ganglion cell survival

Authors:

Schlieve CR, Lieven CJ, Levin LA.

Journal:

Invest Ophthalmol Vis Sci (2006): 3878

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on October 28, 2026