ReadiUse™ Preactivated HRP NHS ester
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Storage, safety and handling
Certificate of Origin | Download PDF |
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
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See also: Amine Reactive Dyes and Probes for Conjugation, Antibodies and Proteomics, Antibody and Protein Labeling, Bioconjugation, Chemical Reagents, Dyes by Functional Group, Horseradish Peroxidase (HRP) and Poly-HRP, Immunohistochemistry (IHC)
ReadiUse™ Preactivated HRP NHS ester is the mono NHS ester of HRP. It can be used to readily label proteins (such as antibodies) or other biologicals molecules that have an amino group. ReadiUse™ Preactivated HRP NHS ester is robust and easy to use. It can be used to label antibodies with a simple mixing, i.e. the basic solution of an antibody (pH 8.5-9.0) can be directly mixed with the HRP NHS ester, and the reaction mixture is shaken for 1-2 hours. In most cases, the resulted solution can be directly used for ELISA assays without further purification. ReadiUse™ Preactivated HRP NHS ester provides the most robust method to perform the conjugation of antibodies with HRP. Any suitable amount of antibody (>1 mg/mL) can be readily conjugated to the preactivated HRP NHS ester without the need for purification.
Example protocol
SAMPLE EXPERIMENTAL PROTOCOL
- Antibody (IgG) ~100 µg in 20 µL~50µL of 25mM Sodium Bicarbonate Buffer (pH=8.5).
- Transfer IgG solution into HRP-SE vial, mix well.
- Reaction for 1~2 hours at room temperature, gently shaking during the reaction is preferred.
- Add equal volume of 100mM Tris-HCl Buffer (pH=7.5)+ 0.5% BSA.
- Store the mixture at 4oC, and it is ready to use. Note: If the target protein solution contains other proteins (such as BSA), purify the protein solution or it will interfere with the labeling reaction. Note: Small molecules such as sodium azide need to be removed. Note: Tris buffer and other amine compounds will also interfere with the labeling reaction.
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Citations
View all 12 citations: Citation Explorer
Florfenicol binding to molecularly imprinted polymer nanoparticles in model and real samples
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Journal: Nanomaterials (2020): 306
Authors: Caro, Nelson and Bruna, Tamara and Guerreiro, Antonio and Alvarez-Tejos, Paola and Garret{\'o}n, Virginia and Piletsky, Sergey and Gonz{\'a}lez-Casanova, Jorge and Rojas-G{\'o}mez, Diana and Ehrenfeld, Nicole
Journal: Nanomaterials (2020): 306
New method for immobilising diverse proteins onto cubic micro-protein polyhedrin crystals
Authors: Yuasa, Haruna and Kotani, Eiji and Mori, Hajime and Takaki, Keiko
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Authors: Yuasa, Haruna and Kotani, Eiji and Mori, Hajime and Takaki, Keiko
Journal: Protein expression and purification (2019): 105531
Assessment of Tofacitinib and Ruxolitinib and their Anti Inflammatory Effects on Myeloperoxidase
Authors: Milton, Amber
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Authors: Milton, Amber
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Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA
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Journal: Analytical Chemistry (2017)
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Journal: Analytical Chemistry (2017)
Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
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Myeloperoxidase--Hepatocyte--Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
Authors: Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W
Journal: Antioxidants & redox signaling (2015): 1255--1269
Authors: Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W
Journal: Antioxidants & redox signaling (2015): 1255--1269
Myeloperoxidase Nuclear Imaging for Epileptogenesis
Authors: Zhang, Yinian and Seeburg, Daniel P and Pulli, Benjamin and Wojtkiewicz, Gregory R and Bure, Lionel and Atkinson, Wendy and Schob, Stefan and Iwamoto, Yoshiko and Ali, Muhammad and Zhang, Wei and others, undefined
Journal: Radiology (2015): 822--830
Authors: Zhang, Yinian and Seeburg, Daniel P and Pulli, Benjamin and Wojtkiewicz, Gregory R and Bure, Lionel and Atkinson, Wendy and Schob, Stefan and Iwamoto, Yoshiko and Ali, Muhammad and Zhang, Wei and others, undefined
Journal: Radiology (2015): 822--830
Ordered cleavage of myeloperoxidase ester bonds releases active site heme leading to inactivation of myeloperoxidase by benzoic acid hydrazide analogs
Authors: Huang, Jiansheng and Smith, Forrest and Panizzi, Peter
Journal: Archives of biochemistry and biophysics (2014): 74--85
Authors: Huang, Jiansheng and Smith, Forrest and Panizzi, Peter
Journal: Archives of biochemistry and biophysics (2014): 74--85
Raising the shields: PCR in the presence of metallic surfaces protected by tailor-made coatings
Authors: Scherag, Frank D and Br, undefined and stetter, Thomas and Rühe, Jürgen
Journal: Colloids and Surfaces B: Biointerfaces (2014): 576--582
Authors: Scherag, Frank D and Br, undefined and stetter, Thomas and Rühe, Jürgen
Journal: Colloids and Surfaces B: Biointerfaces (2014): 576--582
Measuring myeloperoxidase activity in biological samples
Authors: Pulli, Benjamin and Ali, Muhammad and Forghani, Reza and Schob, Stefan and Hsieh, Kevin LC and Wojtkiewicz, Gregory and Linnoila, Jenny J and Chen, John W
Journal: PLoS One (2013): e67976
Authors: Pulli, Benjamin and Ali, Muhammad and Forghani, Reza and Schob, Stefan and Hsieh, Kevin LC and Wojtkiewicz, Gregory and Linnoila, Jenny J and Chen, John W
Journal: PLoS One (2013): e67976
References
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Application notes
A New Protein Crosslinking Method for Labeling and Modifying Antibodies
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)
Abbreviation of Common Chemical Compounds Related to Peptides
Bright Tide Fluor™-Based Fluorescent Peptides and Their Applications In Drug Discovery and Disease Diagnosis
FITC (Fluorescein isothiocyanate)
Fluorescein isothiocyanate (FITC)