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Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*

Horseradish Peroxidase (HRP) is a small molecule (MW ~40 KD) that is widely used in a variety of biological detections. HRP conjugates are extensively used as secondary detection reagents in ELISAs, immuno-histochemical techniques, Northern, Southern and Western blot analyses. Due to its small size, it rarely causes steric hindrance problem with antibody/antigen complex formation. It is usually conjugated to an antibody in a 4:1 ratio. Additionally, HRP is inexpensive compared to other labeling enzymes. The major disadvantage associated with peroxidase is their low tolerance to many preservatives such as sodium azide that inactivates peroxidase activity even at low concentration. Our Amplite® Fluorimetric ELISA Assay Kit contains all the essential components including our fluorogenic Amplite® Red HRP substrate for ELISA detection. The kit provides an optimized assay protocol that is compatible with HTS liquid handling, as little as 10 pg of a monoclonal antibody/well of a microplate can be detected. Its signal can be easily read by either fluorescence microplate reader or absorbance microplate reader. It can be used for assays that detect goat anti-mouse IgG as the secondary detection agent.

Example protocol

AT A GLANCE

Protocol summary

  1. Prepare ELISA plate
  2. Prepare peroxidase working solution
  3. Add 100 µL/well of peroxidase working solution into the ELISA plate
  4. Incubate at room temperature for 15 - 60 minutes
  5. Monitor fluorescence intensity at Ex/Em = 540/590 nm

Important notes
Thaw all the kit components at room temperature before starting the experiment.

PREPARATION OF STOCK SOLUTION

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles.

1. Amplite™ Red Peroxidase Substrate stock solution (200X):
Add 250 µL of DMSO (Component D) into the vial of Amplite™ Red Peroxidase Substrate (Component A). Note: 50 µL of the Amplite™ Red peroxidase substrate stock solution is enough for 1 plate.

2. H2O2 stock solution (20 mM):
Add 22.7 µL of 3% H2O2 (0.88 M, Component B) into 977 µL of Assay Buffer (Component C). Note: The diluted H2O2 stock solution is not stable. The unused portion should be discarded.

PREPARATION OF STANDARD SOLUTION

Mouse IgG standard

For convenience, use the Serial Dilution Planner: https://www.aatbio.com/tools/serial-dilution/11540

Mouse IgG (not included) can be used to generate a standard curve. Dilute total mouse IgG with 0.2 M sodium bicarbonate buffer (pH 9.4) into microplate, using an initial concentration of 3 ug/mL and 1:3 dilution factor.

PREPARATION OF WORKING SOLUTION

1. Goat anti-mouse IgG-HRP conjugate working solution:
Add 2 µL of goat anti-mouse IgG-HRP conjugate (Component E) to 10 mL of PBS with 1% BSA (PBS-BSA, not included). Note: 10 mL of goat anti-mouse IgG-HRP conjugate working solution is enough for 1 plate. The concentration of this goat anti-mouse IgG-HRP conjugate working solution is recommended as an initial concentration to try. The optimal concentration for each particular application should be determined empirically.

2. Peroxidase working solution:
Add 50 μL of Amplite™ Red Peroxidase Substrate stock solution (200X) and 100 μL of H2O2 stock solution (20 mM) into 9.85 mL of Assay Buffer (Component C) to make a total volume of 10 mL Peroxidase working solution. Keep from light.

SAMPLE EXPERIMENTAL PROTOCOL

Prepare ELISA plate:

  1. Perform all necessary ELISA preparation steps.

  2. Wash the ELISA wells three times with PBS containing 0.02% to 0.05% Tween® 20 (PBS-Tween) and drain.

  3. Add 100 µL of prepared goat anti-mouse IgG-HRP conjugate working solution into each well.

  4. Incubate at room temperature for 30 minutes. Drain off the HRP conjugate.

  5. Wash the wells three times with PBS-Tween and drain.

Run peroxidase assay in ELISA plate:

  1. Add 100 µL of peroxidase working solution into each drained microplate well containing the samples and controls.

  2. Incubate the reaction at room temperature for 30 minutes or longer, protected from light.

  3. Monitor the fluorescence increase with a fluorescence plate reader at excitation 530 - 570 nm (optimal at 540 nm) and emission 590 - 600 nm. Note: The plate can also be read by an absorbance microplate reader at the wavelength of 576 ± 5 nm. However, the absorption detection has lower sensitivity compared to fluorescence reading.

Spectrum

Citations

View all 11 citations: Citation Explorer
The combination of circRNA-UMAD1 and Galectin-3 in peripheral circulation is a co-biomarker for predicting lymph node metastasis of thyroid carcinoma
Authors: Yu, Wenbin and Ma, Bo and Zhao, Wei and Liu, Jingtao and Yu, Hao and Tian, Zhihua and Fan, Zhiqin and Han, Haibo
Journal: American Journal of Translational Research (2020): 5399
Patterned Photonic Nitrocellulose for Pseudo-Paper ELISA
Authors: Chi, Junjie and Gao, Bingbing and Sun, Mi and Zhang, Fengling and Su, Enben and Liu, Hong and Gu, Zhongze
Journal: Analytical Chemistry (2017)
Spinal Cord Inflammation: Molecular Imaging after Thoracic Aortic Ischemia Reperfusion Injury
Authors: Albadawi, Hassan and Chen, John W and Oklu, Rahmi and Wu, Yue and Wojtkiewicz, Gregory and Pulli, Benjamin and Milner, John D and Cambria, Richard P and Watkins, Michael T
Journal: Radiology (2016): 152222
Myeloperoxidase--Hepatocyte--Stellate Cell Cross Talk Promotes Hepatocyte Injury and Fibrosis in Experimental Nonalcoholic Steatohepatitis
Authors: Pulli, Benjamin and Ali, Muhammad and Iwamoto, Yoshiko and Zeller, Matthias WG and Schob, Stefan and Linnoila, Jenny J and Chen, John W
Journal: Antioxidants & redox signaling (2015): 1255--1269

References

View all 61 references: Citation Explorer
Kinetic analysis of semisynthetic peroxidase enzymes containing a covalent DNA-heme adduct as the cofactor
Authors: Fruk L, Muller J, Niemeyer CM.
Journal: Chemistry (2006): 7448
Real-time assessment of spatial and temporal coupled catalysis within polyelectrolyte microcapsules containing coimmobilized glucose oxidase and peroxidase
Authors: Stein EW, Volodkin DV, McShane MJ, Sukhorukov GB.
Journal: Biomacromolecules (2006): 710
O2 Delivery and Redox State are Determinants of Compartment-specific Reactive Oxygen Species in Myocardial Reperfusion
Authors: Stoner JD, Clanton TL, Aune SE, Angelos MG.
Journal: Am J Physiol Heart Circ Physiol. (2006)
Fibrillar beta-amyloid peptide Abeta1-40 activates microglial proliferation via stimulating TNF-alpha release and H2O2 derived from NADPH oxidase: a cell culture study
Authors: Jekabsone A, M and er PK, Tickler A, Sharpe M, Brown GC.
Journal: J Neuroinflammation (2006): 24
Biochemical activity of reactive oxygen species scavengers do not predict retinal ganglion cell survival
Authors: Schlieve CR, Lieven CJ, Levin LA.
Journal: Invest Ophthalmol Vis Sci (2006): 3878
Page updated on October 6, 2024

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Spectral properties

Excitation (nm)

571

Emission (nm)

584

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501

Platform

Fluorescence microplate reader

Excitation540 nm
Emission590 nm
Cutoff570 nm
Recommended plateSolid black

Components

Detection of mouse total IgG using the Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP ELISA Kit. Mouse IgG was diluted into 3 µg/mL and made 1 to 3 serial dilutions in 0.2 M sodium bicarbonate buffer, pH 9.4. 100 µL/well serial dilutions were coated into a solid black 96-well plate at 4 °C overnight, and blocked with 3% milk in PBS and 0.02% Tween-20 at 4 °C overnight. The wells were washed and assayed by using the reagents. 1 to 5000 dilutions of goat anti-mouse IgG-HRP conjugate were used. The reactions were incubated for 10 to 60 minutes and then measured for fluorescence at Ex/Em = 540/590 nm using Gemini fluorescence microplate reader (Molecular Devices).
Detection of mouse total IgG using the Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP ELISA Kit. Mouse IgG was diluted into 3 µg/mL and made 1 to 3 serial dilutions in 0.2 M sodium bicarbonate buffer, pH 9.4. 100 µL/well serial dilutions were coated into a solid black 96-well plate at 4 °C overnight, and blocked with 3% milk in PBS and 0.02% Tween-20 at 4 °C overnight. The wells were washed and assayed by using the reagents. 1 to 5000 dilutions of goat anti-mouse IgG-HRP conjugate were used. The reactions were incubated for 10 to 60 minutes and then measured for fluorescence at Ex/Em = 540/590 nm using Gemini fluorescence microplate reader (Molecular Devices).
Detection of mouse total IgG using the Amplite® Fluorimetric Goat Anti-Mouse IgG-HRP ELISA Kit. Mouse IgG was diluted into 3 µg/mL and made 1 to 3 serial dilutions in 0.2 M sodium bicarbonate buffer, pH 9.4. 100 µL/well serial dilutions were coated into a solid black 96-well plate at 4 °C overnight, and blocked with 3% milk in PBS and 0.02% Tween-20 at 4 °C overnight. The wells were washed and assayed by using the reagents. 1 to 5000 dilutions of goat anti-mouse IgG-HRP conjugate were used. The reactions were incubated for 10 to 60 minutes and then measured for fluorescence at Ex/Em = 540/590 nm using Gemini fluorescence microplate reader (Molecular Devices).