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Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*

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Physical properties
Molecular weight177.16
Spectral properties
Excitation (nm)351
Emission (nm)415
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Luminol [3-Aminophthalhydrazide] is a luminescent peroxidase substrate. To exhibit its luminescence, luminol must be activated with an oxidant. HRP catalyzes the decomposition of hydrogen peroxide. When luminol reacts with the hydroxide ion, a dianion is formed. The oxygen produced from the hydrogen peroxide then reacts with the luminol dianion. The product of this reaction, an organic peroxide, is very unstable and is made by the loss of a nitrogen, the change of electrons from excited state to ground state, and the emission of energy as a photon. This emission produces the blue glow.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Luminol [3-Aminophthalhydrazide] *CAS 521-31-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM564.462 µL2.822 mL5.645 mL28.223 mL56.446 mL
5 mM112.892 µL564.462 µL1.129 mL5.645 mL11.289 mL
10 mM56.446 µL282.231 µL564.462 µL2.822 mL5.645 mL

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Spectral properties

Excitation (nm)351
Emission (nm)415



View all 4 citations: Citation Explorer
Linker histone H1. 2 and H1. 4 affect the neutrophil lineage determination
Authors: Sollberger, Gabriel and Streeck, Robert and Apel, Falko and Caffrey, Brian Edward and Skoultchi, Arthur I and Zychlinsky, Arturo
Journal: Elife (2020): e52563
Linker Histone H1 subtypes specifically regulate neutrophil differentiation
Authors: Sollberger, Gabriel and Streeck, Robert and Caffrey, Brian Edward and Zychlinsky, Arturo
Journal: bioRxiv (2019): 763482
Gasdermin D plays a vital role in the generation of neutrophil extracellular traps
Authors: Sollberger, Gabriel and Choidas, Axel and Burn, Garth Lawrence and Habenberger, Peter and Di Lucrezia, Raffaella and Kordes, Susanne and Menninger, Sascha and Eickhoff, Jan and Nussbaumer, Peter and Klebl, Bert and others,
Journal: Science immunology (2018): eaar6689


View all 14 references: Citation Explorer
Luminol-hydrogen peroxide chemiluminescence produced by sweet potato peroxidase
Authors: Alpeeva IS, Yu Sakharov I.
Journal: Luminescence. (2006)
Ultrasensitive assay of azithromycin in medicine and bio-fluids based on its enhanced luminol-H(2)O(2) chemiluminescence reaction using flow injection technique
Authors: Song Z, Wang C.
Journal: Bioorg Med Chem (2003): 5375
Application of an enhanced luminol chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H-imidazol-2-yl]phenylboronic acid to photographic detection of horseradish peroxidase on a membrane
Authors: Kuroda N, Murasaki N, Wada M, Nakashima K.
Journal: Luminescence (2001): 167
Comparative studies of the chemiluminescent horseradish peroxidase-catalysed peroxidation of acridan (GZ-11) and luminol reactions: effect of pH and scavengers of reactive oxygen species on the light intensity of these systems
Authors: Osman AM, Zomer G, Laane C, Hilhorst R.
Journal: Luminescence (2000): 189
New phenylboronic acid derivatives as enhancers of the luminol-H(2)O(2)-horseradish peroxidase chemiluminescence reaction
Authors: Kuroda N, Kawazoe K, Nakano H, Wada M, Nakashima K.
Journal: Luminescence (1999): 361
What do we measure with luminol-, lucigenin- and penicillin-amplified chemiluminescence? 1. Investigations with hydrogen peroxide and sodium hypochlorite
Authors: Rost M, Karge E, Klinger W.
Journal: J Biolumin Chemilumin (1998): 355
Enhancer effect of fluorescein on the luminol-H2O2-horseradish peroxidase chemiluminescence: energy transfer process
Authors: Navas Diaz A, Gonzalez Garcia JA, Lovillo J.
Journal: J Biolumin Chemilumin (1997): 199
Synthesis and characterization of 4-iodophenylboronic acid: a new enhancer for the horseradish peroxidase-catalyzed chemiluminescent oxidation of luminol
Authors: Kricka LJ, Cooper M, Ji X.
Journal: Anal Biochem (1996): 119
Assay of cholinesterase using a pro-enhancer of the luminol-H2O2-horseradish peroxidase reaction
Authors: Navas Diaz A, Garcia Sanchez F, Gonzalez Garcia JA, Bracho Del Rio V.
Journal: J Biolumin Chemilumin (1995): 285
4-Phenylylboronic acid: a new type of enhancer for the horseradish peroxidase catalysed chemiluminescent oxidation of luminol
Authors: Kricka LJ, Ji X.
Journal: J Biolumin Chemilumin (1995): 49