Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*

Luminol

3-Aminophthalhydrazide; CAS 521-31-3

Luminol [3-Aminophthalhydrazide] is a luminescent peroxidase substrate. To exhibit its luminescence, luminol must be activated with an oxidant. HRP catalyzes the decomposition of hydrogen peroxide. When luminol reacts with the hydroxide ion, a dianion is formed. The oxygen produced from the hydrogen peroxide then reacts with the luminol dianion. The product of this reaction, an organic peroxide, is very unstable and is made by the loss of a nitrogen, the change of electrons from excited state to ground state, and the emission of energy as a photon. This emission produces the blue glow.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
11050	1 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Luminol [3-Aminophthalhydrazide] *CAS 521-31-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	564.462 µL	2.822 mL	5.645 mL	28.223 mL	56.446 mL
5 mM	112.892 µL	564.462 µL	1.129 mL	5.645 mL	11.289 mL
10 mM	56.446 µL	282.231 µL	564.462 µL	2.822 mL	5.645 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	177.16 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Linker histone H1. 2 and H1. 4 affect the neutrophil lineage determination

Authors:

Sollberger, Gabriel and Streeck, Robert and Apel, Falko and Caffrey, Brian Edward and Skoultchi, Arthur I and Zychlinsky, Arturo

Journal:

Elife (2020): e52563

Transaldolase 1 is required for Neutrophil Extracellular Trap (NET) Formation

Authors:

Morath, Jakob Paul

Linker Histone H1 subtypes specifically regulate neutrophil differentiation

Authors:

Sollberger, Gabriel and Streeck, Robert and Caffrey, Brian Edward and Zychlinsky, Arturo

Journal:

bioRxiv (2019): 763482

Gasdermin D plays a vital role in the generation of neutrophil extracellular traps

Authors:

Sollberger, Gabriel and Choidas, Axel and Burn, Garth Lawrence and Habenberger, Peter and Di Lucrezia, Raffaella and Kordes, Susanne and Menninger, Sascha and Eickhoff, Jan and Nussbaumer, Peter and Klebl, Bert and others,

Journal:

Science immunology (2018): eaar6689

References

View all 14 references: Citation Explorer

Luminol-hydrogen peroxide chemiluminescence produced by sweet potato peroxidase

Authors:

Alpeeva IS, Yu Sakharov I.

Journal:

Luminescence. (2006)

Ultrasensitive assay of azithromycin in medicine and bio-fluids based on its enhanced luminol-H(2)O(2) chemiluminescence reaction using flow injection technique

Authors:

Song Z, Wang C.

Journal:

Bioorg Med Chem (2003): 5375

Application of an enhanced luminol chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H-imidazol-2-yl]phenylboronic acid to photographic detection of horseradish peroxidase on a membrane

Authors:

Kuroda N, Murasaki N, Wada M, Nakashima K.

Journal:

Luminescence (2001): 167

Comparative studies of the chemiluminescent horseradish peroxidase-catalysed peroxidation of acridan (GZ-11) and luminol reactions: effect of pH and scavengers of reactive oxygen species on the light intensity of these systems

Authors:

Osman AM, Zomer G, Laane C, Hilhorst R.

Journal:

Luminescence (2000): 189

New phenylboronic acid derivatives as enhancers of the luminol-H(2)O(2)-horseradish peroxidase chemiluminescence reaction

Authors:

Kuroda N, Kawazoe K, Nakano H, Wada M, Nakashima K.

Journal:

Luminescence (1999): 361

Physical properties

Molecular weight	177.16
Solvent	DMSO

Spectral properties

Excitation (nm)	351
Emission (nm)	415

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	521-31-3

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