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Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*
Luminol [3-Aminophthalhydrazide] is a luminescent peroxidase substrate. To exhibit its luminescence, luminol must be activated with an oxidant. HRP catalyzes the decomposition of hydrogen peroxide. When luminol reacts with the hydroxide ion, a dianion is formed. The oxygen produced from the hydrogen peroxide then reacts with the luminol dianion. The product of this reaction, an organic peroxide, is very unstable and is made by the loss of a nitrogen, the change of electrons from excited state to ground state, and the emission of energy as a photon. This emission produces the blue glow.
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Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Luminol [3-Aminophthalhydrazide] *CAS 521-31-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 564.462 µL | 2.822 mL | 5.645 mL | 28.223 mL | 56.446 mL |
| 5 mM | 112.892 µL | 564.462 µL | 1.129 mL | 5.645 mL | 11.289 mL |
| 10 mM | 56.446 µL | 282.231 µL | 564.462 µL | 2.822 mL | 5.645 mL |
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Citations
View all 4 citations: Citation Explorer
Linker histone H1. 2 and H1. 4 affect the neutrophil lineage determination
Authors: Sollberger, Gabriel and Streeck, Robert and Apel, Falko and Caffrey, Brian Edward and Skoultchi, Arthur I and Zychlinsky, Arturo
Journal: Elife (2020): e52563
Authors: Sollberger, Gabriel and Streeck, Robert and Apel, Falko and Caffrey, Brian Edward and Skoultchi, Arthur I and Zychlinsky, Arturo
Journal: Elife (2020): e52563
Transaldolase 1 is required for Neutrophil Extracellular Trap (NET) Formation
Authors: Morath, Jakob Paul
Journal: (2020)
Authors: Morath, Jakob Paul
Journal: (2020)
Linker Histone H1 subtypes specifically regulate neutrophil differentiation
Authors: Sollberger, Gabriel and Streeck, Robert and Caffrey, Brian Edward and Zychlinsky, Arturo
Journal: bioRxiv (2019): 763482
Authors: Sollberger, Gabriel and Streeck, Robert and Caffrey, Brian Edward and Zychlinsky, Arturo
Journal: bioRxiv (2019): 763482
Gasdermin D plays a vital role in the generation of neutrophil extracellular traps
Authors: Sollberger, Gabriel and Choidas, Axel and Burn, Garth Lawrence and Habenberger, Peter and Di Lucrezia, Raffaella and Kordes, Susanne and Menninger, Sascha and Eickhoff, Jan and Nussbaumer, Peter and Klebl, Bert and others,
Journal: Science immunology (2018): eaar6689
Authors: Sollberger, Gabriel and Choidas, Axel and Burn, Garth Lawrence and Habenberger, Peter and Di Lucrezia, Raffaella and Kordes, Susanne and Menninger, Sascha and Eickhoff, Jan and Nussbaumer, Peter and Klebl, Bert and others,
Journal: Science immunology (2018): eaar6689
References
View all 14 references: Citation Explorer
Luminol-hydrogen peroxide chemiluminescence produced by sweet potato peroxidase
Authors: Alpeeva IS, Yu Sakharov I.
Journal: Luminescence. (2006)
Authors: Alpeeva IS, Yu Sakharov I.
Journal: Luminescence. (2006)
Ultrasensitive assay of azithromycin in medicine and bio-fluids based on its enhanced luminol-H(2)O(2) chemiluminescence reaction using flow injection technique
Authors: Song Z, Wang C.
Journal: Bioorg Med Chem (2003): 5375
Authors: Song Z, Wang C.
Journal: Bioorg Med Chem (2003): 5375
Application of an enhanced luminol chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H-imidazol-2-yl]phenylboronic acid to photographic detection of horseradish peroxidase on a membrane
Authors: Kuroda N, Murasaki N, Wada M, Nakashima K.
Journal: Luminescence (2001): 167
Authors: Kuroda N, Murasaki N, Wada M, Nakashima K.
Journal: Luminescence (2001): 167
Comparative studies of the chemiluminescent horseradish peroxidase-catalysed peroxidation of acridan (GZ-11) and luminol reactions: effect of pH and scavengers of reactive oxygen species on the light intensity of these systems
Authors: Osman AM, Zomer G, Laane C, Hilhorst R.
Journal: Luminescence (2000): 189
Authors: Osman AM, Zomer G, Laane C, Hilhorst R.
Journal: Luminescence (2000): 189
New phenylboronic acid derivatives as enhancers of the luminol-H(2)O(2)-horseradish peroxidase chemiluminescence reaction
Authors: Kuroda N, Kawazoe K, Nakano H, Wada M, Nakashima K.
Journal: Luminescence (1999): 361
Authors: Kuroda N, Kawazoe K, Nakano H, Wada M, Nakashima K.
Journal: Luminescence (1999): 361
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