Luminol [3-Aminophthalhydrazide] CAS 521-31-3

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Physical properties

Molecular weight	177.16
Solvent	DMSO

Spectral properties

Excitation (nm)	355
Emission (nm)	412

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

521-31-3

Molecular weight

177.16

Excitation (nm)

355

Emission (nm)

412

Luminol [3-Aminophthalhydrazide] is a luminescent peroxidase substrate. To exhibit its luminescence, luminol must be activated with an oxidant. HRP catalyzes the decomposition of hydrogen peroxide. When luminol reacts with the hydroxide ion, a dianion is formed. The oxygen produced from the hydrogen peroxide then reacts with the luminol dianion. The product of this reaction, an organic peroxide, is very unstable and is made by the loss of a nitrogen, the change of electrons from excited state to ground state, and the emission of energy as a photon. This emission produces the blue glow.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Luminol [3-Aminophthalhydrazide] *CAS 521-31-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	564.462 µL	2.822 mL	5.645 mL	28.223 mL	56.446 mL
5 mM	112.892 µL	564.462 µL	1.129 mL	5.645 mL	11.289 mL
10 mM	56.446 µL	282.231 µL	564.462 µL	2.822 mL	5.645 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Excitation (nm)	355
Emission (nm)	412

Images

Figure 1. Chemical structure for Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*

Citations

View all 4 citations: Citation Explorer

Linker histone H1. 2 and H1. 4 affect the neutrophil lineage determination
Authors: Sollberger, Gabriel and Streeck, Robert and Apel, Falko and Caffrey, Brian Edward and Skoultchi, Arthur I and Zychlinsky, Arturo
Journal: Elife (2020): e52563

Transaldolase 1 is required for Neutrophil Extracellular Trap (NET) Formation
Authors: Morath, Jakob Paul
Journal: (2020)

Linker Histone H1 subtypes specifically regulate neutrophil differentiation
Authors: Sollberger, Gabriel and Streeck, Robert and Caffrey, Brian Edward and Zychlinsky, Arturo
Journal: bioRxiv (2019): 763482

Gasdermin D plays a vital role in the generation of neutrophil extracellular traps
Authors: Sollberger, Gabriel and Choidas, Axel and Burn, Garth Lawrence and Habenberger, Peter and Di Lucrezia, Raffaella and Kordes, Susanne and Menninger, Sascha and Eickhoff, Jan and Nussbaumer, Peter and Klebl, Bert and others,
Journal: Science immunology (2018): eaar6689

References

View all 14 references: Citation Explorer

Luminol-hydrogen peroxide chemiluminescence produced by sweet potato peroxidase
Authors: Alpeeva IS, Yu Sakharov I.
Journal: Luminescence. (2006)

Ultrasensitive assay of azithromycin in medicine and bio-fluids based on its enhanced luminol-H(2)O(2) chemiluminescence reaction using flow injection technique
Authors: Song Z, Wang C.
Journal: Bioorg Med Chem (2003): 5375

Application of an enhanced luminol chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H-imidazol-2-yl]phenylboronic acid to photographic detection of horseradish peroxidase on a membrane
Authors: Kuroda N, Murasaki N, Wada M, Nakashima K.
Journal: Luminescence (2001): 167

Comparative studies of the chemiluminescent horseradish peroxidase-catalysed peroxidation of acridan (GZ-11) and luminol reactions: effect of pH and scavengers of reactive oxygen species on the light intensity of these systems
Authors: Osman AM, Zomer G, Laane C, Hilhorst R.
Journal: Luminescence (2000): 189

New phenylboronic acid derivatives as enhancers of the luminol-H(2)O(2)-horseradish peroxidase chemiluminescence reaction
Authors: Kuroda N, Kawazoe K, Nakano H, Wada M, Nakashima K.
Journal: Luminescence (1999): 361

What do we measure with luminol-, lucigenin- and penicillin-amplified chemiluminescence? 1. Investigations with hydrogen peroxide and sodium hypochlorite
Authors: Rost M, Karge E, Klinger W.
Journal: J Biolumin Chemilumin (1998): 355

Enhancer effect of fluorescein on the luminol-H2O2-horseradish peroxidase chemiluminescence: energy transfer process
Authors: Navas Diaz A, Gonzalez Garcia JA, Lovillo J.
Journal: J Biolumin Chemilumin (1997): 199

Synthesis and characterization of 4-iodophenylboronic acid: a new enhancer for the horseradish peroxidase-catalyzed chemiluminescent oxidation of luminol
Authors: Kricka LJ, Cooper M, Ji X.
Journal: Anal Biochem (1996): 119

Assay of cholinesterase using a pro-enhancer of the luminol-H2O2-horseradish peroxidase reaction
Authors: Navas Diaz A, Garcia Sanchez F, Gonzalez Garcia JA, Bracho Del Rio V.
Journal: J Biolumin Chemilumin (1995): 285

4-Phenylylboronic acid: a new type of enhancer for the horseradish peroxidase catalysed chemiluminescent oxidation of luminol
Authors: Kricka LJ, Ji X.
Journal: J Biolumin Chemilumin (1995): 49