Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*

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Chemical structure for Luminol [3-Aminophthalhydrazide] *CAS 521-31-3*
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Ex/Em (nm)353/410
CAS #521-31-3
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Category Enzyme Detection
Horseradish Peroxidase (HRP)
Luminol [3-Aminophthalhydrazide] is a luminescent peroxidase substrate. To exhibit its luminescence, luminol must be activated with an oxidant. HRP catalyzes the decomposition of hydrogen peroxide. When luminol reacts with the hydroxide ion, a dianion is formed. The oxygen produced from the hydrogen peroxide then reacts with the luminol dianion. The product of this reaction, an organic peroxide, is very unstable and is made by the loss of a nitrogen, the change of electrons from excited state to ground state, and the emission of energy as a photon. This emission produces the blue glow.

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Luminol [3-Aminophthalhydrazide] *CAS 521-31-3* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

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Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
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Authors: Alpeeva IS, Yu Sakharov I.
Journal: Luminescence. (2006)

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Application of an enhanced luminol chemiluminescence reaction using 4-[4,5-di(2-pyridyl)-1H-imidazol-2-yl]phenylboronic acid to photographic detection of horseradish peroxidase on a membrane
Authors: Kuroda N, Murasaki N, Wada M, Nakashima K.
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Comparative studies of the chemiluminescent horseradish peroxidase-catalysed peroxidation of acridan (GZ-11) and luminol reactions: effect of pH and scavengers of reactive oxygen species on the light intensity of these systems
Authors: Osman AM, Zomer G, Laane C, Hilhorst R.
Journal: Luminescence (2000): 189

New phenylboronic acid derivatives as enhancers of the luminol-H(2)O(2)-horseradish peroxidase chemiluminescence reaction
Authors: Kuroda N, Kawazoe K, Nakano H, Wada M, Nakashima K.
Journal: Luminescence (1999): 361

What do we measure with luminol-, lucigenin- and penicillin-amplified chemiluminescence? 1. Investigations with hydrogen peroxide and sodium hypochlorite
Authors: Rost M, Karge E, Klinger W.
Journal: J Biolumin Chemilumin (1998): 355

Enhancer effect of fluorescein on the luminol-H2O2-horseradish peroxidase chemiluminescence: energy transfer process
Authors: Navas Diaz A, Gonzalez Garcia JA, Lovillo J.
Journal: J Biolumin Chemilumin (1997): 199

Synthesis and characterization of 4-iodophenylboronic acid: a new enhancer for the horseradish peroxidase-catalyzed chemiluminescent oxidation of luminol
Authors: Kricka LJ, Cooper M, Ji X.
Journal: Anal Biochem (1996): 119

4-Phenylylboronic acid: a new type of enhancer for the horseradish peroxidase catalysed chemiluminescent oxidation of luminol
Authors: Kricka LJ, Ji X.
Journal: J Biolumin Chemilumin (1995): 49

Assay of cholinesterase using a pro-enhancer of the luminol-H2O2-horseradish peroxidase reaction
Authors: Navas Diaz A, Garcia Sanchez F, Gonzalez Garcia JA, Bracho Del Rio V.
Journal: J Biolumin Chemilumin (1995): 285

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