iFluor® 840 acid
In vivo fluorescence imaging uses a sensitive camera to detect the fluorescence emission from fluorophores in whole-body living small animals. To overcome the photon attenuation in living tissue, fluorophores with long emission at the infrared (IR) region are generally preferred. Recent advances in imaging strategies and reporter techniques for in vivo fluorescence imaging include novel approaches to improve the specificity and affinity of the probes and to modulate and amplify the signal at target sites for enhanced sensitivity. Further emerging developments aim to achieve high-resolution, multimodality, and lifetime-based in vivo fluorescence imaging. Our iFluor® 830 is designed to label proteins and other biomolecules with infrared fluorescence. Conjugates prepared with iFluor® 830 have excitation and emission in the IR range. iFluor® 840 dye emission is well separated from commonly used far-red fluorophores such as Cy5, Cy7, or allophycocyanin (APC), facilitating multicolor analysis. This fluorophore is also useful for small animal in vivo imaging applications or other imaging applications requiring IR detection.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 840 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 70.126 µL | 350.629 µL | 701.257 µL | 3.506 mL | 7.013 mL |
5 mM | 14.025 µL | 70.126 µL | 140.251 µL | 701.257 µL | 1.403 mL |
10 mM | 7.013 µL | 35.063 µL | 70.126 µL | 350.629 µL | 701.257 µL |
Molarity calculator
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Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
iFluor® 790 acid | 787 | 812 | 2500001 | 0.131 | 0.1 | 0.09 |
iFluor® 800 acid | 801 | 820 | 2500001 | 0.111 | 0.03 | 0.08 |
iFluor® 810 acid | 811 | 822 | 2500001 | 0.051 | 0.09 | 0.15 |
iFluor® 820 acid | 822 | 850 | 2500001 | 0.11 | 0.16 | |
iFluor® 860 acid | 853 | 878 | 2500001 | 0.1 | 0.14 | |
iFluor® 830 acid | 830 | 867 | - | - | - | - |
iFluor® 670 acid | 671 | 682 | 2000001 | 0.551 | 0.03 | 0.033 |
iFluor® 350 acid | 345 | 450 | 200001 | 0.951 | 0.83 | 0.23 |
iFluor® 405 acid | 403 | 427 | 370001 | 0.911 | 0.48 | 0.77 |
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Citations
View all 8 citations: Citation Explorer
Block Face Scanning Electron Microscopy of Fluorescently Labeled Axons Without Using Near Infra-Red Branding
Authors: Maclachlan, C., Sahlender, D. A., Hayashi, S., Molnar, Z., Knott, G.
Journal: Front Neuroanat (2018): 88
Authors: Maclachlan, C., Sahlender, D. A., Hayashi, S., Molnar, Z., Knott, G.
Journal: Front Neuroanat (2018): 88
Bioactive magnetic near Infra-Red fluorescent core-shell iron oxide/human serum albumin nanoparticles for controlled release of growth factors for augmentation of human mesenchymal stem cell growth and differentiation
Authors: Levy, I., Sher, I., Corem-Salkmon, E., Ziv-Polat, O., Meir, A., Treves, A. J., Nagler, A., Kalter-Leibovici, O., Margel, S., Rotenstreich, Y.
Journal: J Nanobiotechnology (2015): 34
Authors: Levy, I., Sher, I., Corem-Salkmon, E., Ziv-Polat, O., Meir, A., Treves, A. J., Nagler, A., Kalter-Leibovici, O., Margel, S., Rotenstreich, Y.
Journal: J Nanobiotechnology (2015): 34
Novel near infra-red fluorescent pH sensors based on 1-aminoperylene bisimides covalently grafted onto poly(acryloylmorpholine)
Authors: Aigner, D., Borisov, S. M., Petritsch, P., Klimant, I.
Journal: Chem Commun (Camb) (2013): 2139-41
Authors: Aigner, D., Borisov, S. M., Petritsch, P., Klimant, I.
Journal: Chem Commun (Camb) (2013): 2139-41
In vitro and ex vivo evaluation of smart infra-red fluorescent caspase-3 probes for molecular imaging of cardiovascular apoptosis
Authors: Debunne, M., Portal, C., Delest, B., Brakenhielm, E., Lallem and , F., Henry, J. P., Ligeret, H., Noack, P., Massonneau, M., Romieu, A., Renard, P. Y., Thuillez, C., Richard, V.
Journal: Int J Mol Imaging (2011): 413290
Authors: Debunne, M., Portal, C., Delest, B., Brakenhielm, E., Lallem and , F., Henry, J. P., Ligeret, H., Noack, P., Massonneau, M., Romieu, A., Renard, P. Y., Thuillez, C., Richard, V.
Journal: Int J Mol Imaging (2011): 413290
Simple sensitive and simultaneous high-performance liquid chromatography method of glucoconjugated and non-glucoconjugated porphyrins and chlorins using near infra-red fluorescence detection
Authors: Canada-Canada, F., Bautista-Sanchez, A., Taverna, M., Prognon, P., Maillard, P., Grierson, D. S., Kasselouri, A.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2005): 166-72
Authors: Canada-Canada, F., Bautista-Sanchez, A., Taverna, M., Prognon, P., Maillard, P., Grierson, D. S., Kasselouri, A.
Journal: J Chromatogr B Analyt Technol Biomed Life Sci (2005): 166-72
References
View all 19 references: Citation Explorer
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Journal: Nanomedicine (2017)
Authors: Hsu, Ching-Yun and Chen, Chun-Han and Aljuffali, Ibrahim A and Dai, You-Shan and Fang, Jia-You
Journal: Nanomedicine (2017)
A protocol for imaging alternative splicing regulation in vivo using fluorescence reporters in transgenic mice
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Journal: Nat Protoc (2007): 2166
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Journal: Nat Protoc (2007): 2166
A target cell-specific activatable fluorescence probe for in vivo molecular imaging of cancer based on a self-quenched avidin-rhodamine conjugate
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Journal: Cancer Res (2007): 2791
Fluorescence imaging in vivo: recent advances
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Journal: Curr Opin Biotechnol (2007): 17
Authors: Rao J, Dragulescu-Andrasi A, Yao H.
Journal: Curr Opin Biotechnol (2007): 17
Ex vivo fluorescence imaging of normal and malignant urothelial cells to enhance early diagnosis
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