iFluor® 488 Styramide™

iFluor® 488 Styramide™ is a green-fluorescent signal amplification reagent for sensitive detection of low-abundance targets in cells and tissues using HRP-mediated labeling.

Enhanced Signal Amplification: PSA™ technology provides manufacturer-reported 10–50-fold higher fluorescence signals than conventional TSA reagents.
Spectral Properties: Excitation at 491 nm and emission at 516 nm.
HRP-Mediated Labeling: Enables localized covalent deposition of fluorescent labels at target sites.
Filter Compatibility: Supports fluorescence imaging using standard FITC filter sets.
Applications: Suitable for amplified immunofluorescence, immunocytochemistry, and immunohistochemistry.

Fluorescence IHC of formaldehyde-fixed, paraffin-embedded human lung adenocarcinoma positive tissue using PSA<strong> ™ </strong> and TSA amplified methods. Human lung adenocarcinoma positive tissue sections were stained with rabbit anti-EpCam antibody and then incubated with polyHRP-labeled Goat anti-Rabbit IgG secondary antibody followed by iFluor® 488 Styramide™ (Cat#45020) or Alexa Fluor® 488 tyramide stain respectively.  Images showed that iFluor 488™ PSA™ super signal amplification can increase the sensitivity of fluorescence IHC over Alexa Fluor® 488 TSA method.

Protocol

SDS

Catalog	Size	Price	Quantity
45020	100 Slides	Price

Overview

Power Styramide™ Signal Amplification (PSA™) system is one of the most sensitive methods that can detect extremely low-abundance targets in cells and tissues with improved fluorescence signal 10-50 times higher than the widely used tyramide (TSA) reagents. In combination with our superior iFluor® dyes that have higher florescence intensity, increased photostability and enhanced water solubility, the iFluor® dye-labeled Styramide™ conjugates can generate fluorescence signal with significantly higher precision and sensitivity (more than 100 times) than standard ICC/IF/IHC. PSA™ utilizes the catalytic activity of horseradish peroxidase (HRP) for covalent deposition of fluorophores in situ. PSA™ radicals have much higher reactivity than tyramide radicals, making the PSA™ system much faster, more robust and sensitive than the traditional TSA reagents. Compared to tyramide reagents, the Styramide™ conjugates have ability to label the target at higher efficiency and thus generate significantly higher fluorescence signal. Styramide™ conjugates also allow significantly less consumption of primary antibody compared to standard directly conjugate method or tyramide amplification with the same level of sensitivity. iFluor® 488 Styramide is a superior replacement for Alexa Fluor 488 tyramide, Opal 520 or other spectrally similar fluorescent tyramide conjugates or TSA reagents.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 488 Styramide™ to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.34 µL	481.7 µL	963.4 µL	4.817 mL	9.634 mL
5 mM	19.268 µL	96.34 µL	192.68 µL	963.4 µL	1.927 mL
10 mM	9.634 µL	48.17 µL	96.34 µL	481.7 µL	963.4 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1037.99 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

Tyramide signal amplification-based detection system: A novel approach to improve detection efficiency for circulating tumor cells

Authors:

Chen, Lin and Yang, Zhonglin and Lu, Yue and Li, Shan and Tang, Dongjiang and Zhang, Lei

Journal:

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy (2025): 126898

Hypothalamic PNOC/NPY neurons constitute mediators of leptin-controlled energy homeostasis

Authors:

Solheim, Marie H and Stroganov, Sima and Chen, Weiyi and Subagia, P Sicilia and Bauder, Corinna A and Wnuk-Lipinski, Daria and Del R{\'\i}o-Mart{\'\i}n, Almudena and Sotelo-Hitschfeld, Tamara and Beddows, Cait A and Klemm, Paul and others,

Journal:

Cell (2025)

Membrane progesterone receptor $\gamma$ (paqr5b) is essential for the formation of neurons in the zebrafish olfactory rosette

Authors:

Mustary, Umme Habiba and Maeno, Akiteru and Rahaman, Md Mostafizur and Ali, Md Hasan and Tokumoto, Toshinobu

Journal:

Scientific Reports (2024): 24354

Inhibition of platelet-derived growth factor pathway suppresses tubulointerstitial injury in renal congestion

Authors:

Matsuki, Takuma and Hirose, Takuo and Ohsaki, Yusuke and Shimada, Satoshi and Endo, Akari and Ito, Hiroki and Takahashi, Chika and Yamakoshi, Seiko and Oba-Yabana, Ikuko and Anan, Go and others,

Journal:

Journal of hypertension (2022): 1935--1949

CD95/Fas protects triple negative breast cancer from anti-tumor activity of NK cells

Authors:

Qadir, Abdul S and Gu{\'e}gan, Jean Philippe and Ginestier, Christophe and Chaibi, Assia and Bessede, Alban and Charafe-Jauffret, Emmanuelle and Macario, Manon and Lavou{\'e}, Vincent and de la Motte Rouge, Thibault and Law, Calvin and others,

Journal:

Iscience (2021): 103348

References

View all 50 references: Citation Explorer

Immunofluorescent Staining of Adult Murine Paraffin-Embedded Skeletal Tissue.

Authors:

Felsenthal, Neta and Zelzer, Elazar

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 337-344

Highly Sensitive and Multiplexed In Situ RNA Profiling with Cleavable Fluorescent Tyramide.

Authors:

Xiao, Lu and Labaer, Joshua and Guo, Jia

Journal:

Cells (2021)

Single-cell RNA sequencing of human liver reveals hepatic stellate cell heterogeneity.

Authors:

Payen, Valéry L and Lavergne, Arnaud and Alevra Sarika, Niki and Colonval, Megan and Karim, Latifa and Deckers, Manon and Najimi, Mustapha and Coppieters, Wouter and Charloteaux, Benoît and Sokal, Etienne M and El Taghdouini, Adil

Journal:

JHEP reports : innovation in hepatology (2021): 100278

Multiplexed In Situ Protein Profiling with High-Performance Cleavable Fluorescent Tyramide.

Authors:

Pham, Thai and Liao, Renjie and Labaer, Joshua and Guo, Jia

Journal:

Molecules (Basel, Switzerland) (2021)

Accessibility-dependent topology studies of membrane proteins using a SpyTag/SpyCatcher protein-ligation system.

Authors:

Bae, Yoonji and Lee, Sang Kwon and Chae, Young Chan and Park, Chan Young and Kang, Sebyung

Journal:

International journal of biological macromolecules (2021): 171-178

Physical properties

Molecular weight	1037.99
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 30, 2026