iFluor® 488-Concanavalin A Conjugate

iFluor® 488-Concanavalin A Conjugate is a green-fluorescent lectin probe for detecting cell-surface glycoconjugates and visualizing cellular membranes in live and fixed cells.

Selective Carbohydrate Binding: Recognizes α-D-mannosyl and α-D-glucosyl residues on glycoconjugates.
Spectral Properties: Excitation at 491 nm and emission at 516 nm.
Cell Membrane Labeling: Enables fluorescent staining of cell-surface glycoproteins and glycolipids.
Filter Compatibility: Supports fluorescence imaging using standard FITC filter sets.
Applications: Suitable for live- and fixed-cell staining and glycoconjugate localization.

Live HeLa cells were stained with iFluor® 488-Concanavalin A Conjugate Conjugate at 5 µg/mL for 30 minutes. Image was acquired using fluorescence microscopy using FITC filter set.

Catalog	Size	Price	Quantity
25580	1 mg	Price

Overview

Concanavalin A (ConA) is a lectin that binds specifically to certain structures found in various sugars, glycoproteins and glycolipids. Con A is a well-known T cell mitogen that can activate the immune system, recruit lymphocytes and elicit cytokine production. In addition to its mitogenic activity, ConA can induce programmed cell death via mitochondria-mediated apoptosis and autophagy. ConA has also been reported to activate NFAT (nuclear factor of activated T cells), a family of transcription factors that are important in the development and function of the immune system, including T cell receptor (TCR) engagement. ConA is widely used in biology and biochemistry to characterize glycoproteins and other sugar-containing entities on the surface of various cells. It is also used to purify glycosylated macromolecules in lectin affinity chromatography, as well as to study immune regulation by various immune cells. ConA binds specifically α-D-mannosyl and α-D-glucosyl residues (two hexoses differing only in the alcohol on carbon 2) in terminal position of ramified structures from B-Glycans. It has 4 binding sites, corresponding to the 4 subunits. Concanavalin A (Con A) is one of the most widely used lectins in cell biology. iFluor®488-labeled Concanavalin A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues and might exhibit the brightest green fluorescence.

References

View all 50 references: Citation Explorer

Short-term high-fat diet intake leads to exacerbation of concanavalin A-induced liver injury through the induction of procoagulation state.

Authors:

Nanizawa, Eri and Tamaki, Yuki and Sono, Reika and Miyashita, Rintaro and Hayashi, Yumi and Kanbe, Ayumu and Ito, Hiroyasu and Ishikawa, Tetsuya

Journal:

Biochemistry and biophysics reports (2020): 100736

Montelukast ameliorates Concanavalin A-induced autoimmune hepatitis in mice via inhibiting TNF-α/JNK signaling pathway.

Authors:

El-Kashef, Dalia H and Abdelrahman, Rehab S

Journal:

Toxicology and applied pharmacology (2020): 114931

Acupuncture stimulation attenuates TNF-α production via vagal modulation in the concanavalin A model of hepatitis.

Authors:

Lim, Hee-Don and Kim, Ki-Joong and Jo, Byung Gon and Park, Ji-Yeun and Namgung, Uk

Journal:

Acupuncture in medicine : journal of the British Medical Acupuncture Society (2020): 964528420907338

Deficiency of O-linked-glycosylation regulates activation of T cells and aggravates Concanavalin A-induced liver injury.

Authors:

Hao, Xiaohua and Gao, Meixin and He, Lingling and Ye, Xiaohui and Yang, Junru and Zhang, Fuyang and Liu, Ran and Wei, Hongshan

Journal:

Toxicology (2020): 152411

Nicotine attenuates concanavalin A-induced liver injury in mice by regulating the α7-nicotinic acetylcholine receptor in Kupffer cells.

Authors:

Zhao, Jing and Park, Surim and Kim, Jong-Won and Qi, Jing and Zhou, Zixiong and Lim, Chae Woong and Kim, Bumseok

Journal:

International immunopharmacology (2020): 106071

Physical properties

Molecular weight	N/A
Solvent	Water

Spectral properties

Correction factor (260 nm)	0.21
Correction factor (280 nm)	0.11
Extinction coefficient (cm ^-1 M ^-1)	75000 ¹
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.9 ¹

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

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Page updated on October 23, 2026