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iFluor® 750-Concanavalin A Conjugate

Live HeLa cells were stained with iFluor® 750-Concanavalin A Conjugate at 10 µg/mL for 30 minutes. The image was acquired using fluorescence microscopy equipped with a Cy7 filter set.
Live HeLa cells were stained with iFluor® 750-Concanavalin A Conjugate at 10 µg/mL for 30 minutes. The image was acquired using fluorescence microscopy equipped with a Cy7 filter set.
Live HeLa cells were stained with iFluor® 750-Concanavalin A Conjugate at 10 µg/mL for 30 minutes. The image was acquired using fluorescence microscopy equipped with a Cy7 filter set.
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Physical properties
SolventWater
Spectral properties
Correction Factor (260 nm)0.044
Correction Factor (280 nm)0.039
Correction Factor (565 nm)0.0250
Correction Factor (650 nm)0.1413
Extinction coefficient (cm -1 M -1)2750001
Excitation (nm)757
Emission (nm)779
Quantum yield0.121
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
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Show More (281)

OverviewpdfSDSpdfProtocol


Correction Factor (260 nm)
0.044
Correction Factor (280 nm)
0.039
Correction Factor (565 nm)
0.0250
Correction Factor (650 nm)
0.1413
Extinction coefficient (cm -1 M -1)
2750001
Excitation (nm)
757
Emission (nm)
779
Quantum yield
0.121
iFluor 750-labeled Concanavalin A selectively binds to a-mannopyranosyl and a-glucopyranosyl residues and exhibit the NIR fluorescence. Concanavalin A (ConA) is a lectin, a type of protein that specifically binds to carbohydrates, found in the seeds of the Jack bean plant (Canavalia ensiformis). ConA is widely used as a tool for studying carbohydrate-binding proteins, cell surface glycans, and cellular processes involving glycoproteins and glycolipids. ConA binds specifically to the sugar molecule mannose and glucose, which are commonly found in the carbohydrates of glycoproteins and glycolipids. When added to a biological sample, ConA can be used to selectively isolate and purify glycoproteins or to detect the presence of glycoproteins and glycans in cells and tissues. Additionally, ConA can activate T cells and stimulate cell proliferation and differentiation. ConA has been used in a variety of applications, including cell sorting, immunoassays, and studies of cellular signaling pathways. It has also been investigated as a potential therapeutic agent for the treatment of autoimmune diseases and cancer.

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.044
Correction Factor (280 nm)0.039
Correction Factor (565 nm)0.0250
Correction Factor (650 nm)0.1413
Extinction coefficient (cm -1 M -1)2750001
Excitation (nm)757
Emission (nm)779
Quantum yield0.121

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
iFluor® 488-Concanavalin A Conjugate4915167500010.910.210.11
iFluor® 555-Concanavalin A Conjugate55757010000010.6410.230.14
iFluor® 594-Concanavalin A Conjugate58760320000010.5310.050.04
iFluor® 647-Concanavalin A Conjugate65667025000010.2510.030.03

Images


References


View all 50 references: Citation Explorer
Glucosylceramide synthase regulates hepatocyte repair after concanavalin A-induced immune-mediated liver injury.
Authors: Gan, Jian and Gao, Qin and Wang, Li Li and Tian, Ai Ping and Zhu, Long Dong and Zhang, Li Ting and Zhou, Wei and Mao, Xiao Rong and Li, Jun Feng
Journal: PeerJ (2021): e12138
Carbohydrate-protein template synthesized high mannose loading gold nanoclusters: A powerful fluorescence probe for sensitive Concanavalin A detection and specific breast cancer cell imaging.
Authors: Sha, Qiuyue and Guan, Ruixue and Su, Huiying and Zhang, Liang and Liu, Bi-Feng and Hu, Zhaoyu and Liu, Xin
Journal: Talanta (2020): 121130
Click-Particle Display for Base-Modified Aptamer Discovery.
Authors: Gordon, Chelsea K L and Wu, Diana and Pusuluri, Anusha and Feagin, Trevor A and Csordas, Andrew T and Eisenstein, Michael S and Hawker, Craig J and Niu, Jia and Soh, Hyongsok Tom
Journal: ACS chemical biology (2019): 2652-2662
Water-Soluble Glucosyl Pyrene Photosensitizers: An Intramolecularly Synthesized 2- C-Glucoside and an O-Glucoside.
Authors: Kanamori, Takashi and Matsuyama, Akira and Naito, Hidenori and Tsuga, Yuki and Ozako, Yoshiki and Ogura, Shun-Ichiro and Okazaki, Shigetoshi and Yuasa, Hideya
Journal: The Journal of organic chemistry (2018): 13765-13775
Catalyst-Free Cycloaddition Reaction for the Synthesis of Glyconanoparticles.
Authors: Kong, Na and Xie, Sheng and Zhou, Juan and Menéndez, Margarita and Solís, Dolores and Park, JaeHyeung and Proietti, Giampiero and Ramström, Olof and Yan, Mingdi
Journal: ACS applied materials & interfaces (2016): 28136-28142
Lectin-carbohydrate recognition mechanism of Plasmodium berghei in the midgut of malaria vector Anopheles stephensi using quantum dot as a new approach.
Authors: Basseri, Hamid R and Javazm, Mahdi Salari and Farivar, Leila and Abai, Mohammad R
Journal: Acta tropica (2016): 37-42
Pharmacological Modulation of GluK1 and GluK2 by NETO1, NETO2, and PSD95.
Authors: Li, Baolin and Rex, Elizabeth and Wang, He and Qian, Yuewei and Ogden, Ann Marie and Bleakman, David and Johnson, Kirk W
Journal: Assay and drug development technologies (2016): 131-43
Spectroscopic and nonlinear optical properties of new chalcone fluorescent probes for bioimaging applications: a theoretical and experimental study.
Authors: Krawczyk, Przemysław and Pietrzak, Marek and Janek, Tomasz and Jędrzejewska, Beata and Cysewski, Piotr
Journal: Journal of molecular modeling (2016): 125
Quantitative fluorine NMR to determine carbohydrate density on glyconanomaterials synthesized from perfluorophenyl azide-functionalized silica nanoparticles by click reaction.
Authors: Kong, Na and Zhou, Juan and Park, JaeHyeung and Xie, Sheng and Ramström, Olof and Yan, Mingdi
Journal: Analytical chemistry (2015): 9451-8
Fluorescence turn-on sensing of protein based on mannose functionalized perylene bisimides and its fluorescence imaging.
Authors: Wang, Ke-Rang and An, Hong-Wei and Rong, Rui-Xue and Cao, Zhi-Ran and Li, Xiao-Liu
Journal: Biosensors & bioelectronics (2014): 27-32