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Spectral Properties For Cyanine Dyes: Cy3 and Cy5
| Cy Dye ▲ ▼ | Laser ▲ ▼ | Ex/Em (nm) ▲ ▼ | Filter Set ▲ ▼ | Extinction Coefficient (ε)1 ▲ ▼ | Quantum Yield (Φ)2 ▲ ▼ |
| Cy3 | 532 nm | 554/568 nm | Cy3/TRITC | 150,000 | 0.15 |
| Cy5 | 633-647 nm | 650/669 nm | Cy5 | 250,000 | 0.27 |
| Cy7 | 730 nm | 754/778 nm | Cy7 | 250,000 | 0.3 |
References
This online tool may be cited as follows
MLA | "Quest Database™ Spectral Properties For Cyanine Dyes: Cy3 and Cy5." AAT Bioquest, Inc., 2 May. 2025, https://www.aatbio.com/data-sets/spectral-properties-for-cyanine-dyes-cy3-and-cy5. | |
APA | AAT Bioquest, Inc. (2025, May 2). Quest Database™ Spectral Properties For Cyanine Dyes: Cy3 and Cy5. AAT Bioquest. https://www.aatbio.com/data-sets/spectral-properties-for-cyanine-dyes-cy3-and-cy5. | |
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