iFluor® 555 succinimidyl ester

AAT Bioquest's iFluor® dyes are optimized for labeling proteins, particularly antibodies. These dyes are bright, photostable, and have minimal quenching on proteins. They can be well excited by the major laser lines of fluorescence instruments (e.g., 350, 405, 488, 555, and 633 nm). iFluor® 555 dyes have fluorescence excitation and emission maxima of ~557 nm and ~570 nm respectively. The iFluor® 555 family has spectral properties essentially identical to those of Cy3® (Cy3® is the trademark of GE Healthcare). Compared to Cy3 probes, iFluor® 555 reagents has much stronger fluorescence and higher photostability. Their fluorescence is pH-independent from pH 3 to 11. These spectral characteristics make this new dye family a superior alternative to Cy3®. iFluor® 555 family has become an excellent replacement for Cy3® and Alexa Fluor® 555 labeling dye (Cy3® and Alexa Fluor® are the trademarks of Invitrogen and GE Health Care). iFluor® 555 SE is reasonably stable and shows good reactivity and selectivity with protein amino groups.

HeLa cells were incubated with mouse anti-tubulin followed by AAT’s iFluor<sup>TM</sup> 555 goat anti-mouse IgG conjugate (Red, Right) or goat anti-mouse IgG conjugated with Alexa Fluor<sup>®</sup> 555  (Red, left), respectively. Cell nuclei were stained with Hoechst 33342 (Blue, Cat# 17530).

Protocol

COO

COA

Catalog	Size	Price
1028	1 mg	Price
71028	100 ug	Price
71507	5 mg	Price
71557	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of iFluor® 555 succinimidyl ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	88.868 µL	444.342 µL	888.684 µL	4.443 mL	8.887 mL
5 mM	17.774 µL	88.868 µL	177.737 µL	888.684 µL	1.777 mL
10 mM	8.887 µL	44.434 µL	88.868 µL	444.342 µL	888.684 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1125.26 g/mol	=	mL	x	mM	=	mmol

Citations

View all 6 citations: Citation Explorer

Iron-depleting nutritional immunity controls extracellular bacterial replication in Legionella pneumophila infections

Authors:

Torres-Escobar, Ascenci{\'o}n and Wilkins, Ashley and Ju{\'a}rez-Rodr{\'\i}guez, Mar{\'\i}a D and Circu, Magdalena and Latimer, Brian and Dragoi, Ana-Maria and Ivanov, Stanimir S

Journal:

Nature Communications (2024): 7848

Using F{\"o}rster Resonance Energy Transfer (FRET) to Understand the Ubiquitination Landscape

Authors:

Gill, Jashanjot Kaur and Shaw, Gary

Journal:

ChemBioChem (2024): e202400193

Rapid and Effective Generation of Nanobody Based CARs using PCR and Gibson Assembly

Authors:

De Munter, Stijn and Van Parys, Alexander and Bral, Layla and Ingels, Joline and Goetgeluk, Glenn and Bonte, Sarah and Pille, Melissa and Billiet, Lore and Weening, Karin and Verhee, Annick and others,

Journal:

International journal of molecular sciences (2020): 883

Deep Sequencing Analysis of the Eha-Regulated Transcriptome of Edwardsiella tarda Following Acidification

Authors:

Gao, D and Liu, N and Li, Y and Zhang, Y and Liu, G and others, undefined

Journal:

Metabolomics (Los Angel) (2017): 2153--0769

Suramin inhibits cullin-RING E3 ubiquitin ligases

Authors:

Wu, Kenneth and Chong, Robert A and Yu, Qing and Bai, Jin and Spratt, Donald E and Ching, Kevin and Lee, Chan and Miao, Haibin and Tappin, Inger and Hurwitz, Jerard and others, undefined

Journal:

Proceedings of the National Academy of Sciences (2016): E2011--E2018

References

View all 49 references: Citation Explorer

Sequential ordering among multicolor fluorophores for protein labeling facility via aggregation-elimination based beta-lactam probes

Authors:

Sadhu KK, Mizukami S, Watanabe S, Kikuchi K.

Journal:

Mol Biosyst (2011): 1766

Visualizing dengue virus through Alexa Fluor labeling

Authors:

Zhang S, Tan HC, Ooi EE.

Journal:

J Vis Exp. (2011)

Fluorescent "Turn-on" system utilizing a quencher-conjugated peptide for specific protein labeling of living cells

Authors:

Arai S, Yoon SI, Murata A, Takabayashi M, Wu X, Lu Y, Takeoka S, Ozaki M.

Journal:

Biochem Biophys Res Commun (2011): 211

Neuroanatomical basis of clinical joint application of "Jinggu" (BL 64, a source-acupoint) and "Dazhong" (KI 4, a Luo-acupoint) in the rat: a double-labeling study of cholera toxin subunit B conjugated with Alexa Fluor 488 and 594

Authors:

Cui JJ, Zhu XL, Ji CF, Jing XH, Bai WZ.

Journal:

Zhen Ci Yan Jiu (2011): 262

Simultaneous detection of virulence factors from a colony in diarrheagenic Escherichia coli by a multiplex PCR assay with Alexa Fluor-labeled primers

Authors:

Kuwayama M, Shigemoto N, Oohara S, Tanizawa Y, Yamada H, Takeda Y, Matsuo T, Fukuda S.

Journal:

J Microbiol Methods (2011): 119

Physical properties

Molecular weight	1125.26
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.23
Correction factor (280 nm)	0.14
Extinction coefficient (cm ^-1 M ^-1)	100000 ¹
Excitation (nm)	557
Emission (nm)	570
Quantum yield	0.64 ¹

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on September 23, 2025