10XHis alkyne
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1668.78 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
10XHis Succinimidyl Ester |
10XHis maleimide |
10XHis azide |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 1668.78 |
10XHis alkyne is an excellent building block to make 10XHis conjugates for developing His tag detection probes and purification tools. It can be readily conjugated to a biomolecule that contains an azido group, such as azide-modified antibodies, peptides and oligos. The 10XHis tag is one of the most common His tags and has a molecular weight of ~2kDa. His-tags (i.e., polyhistidine tags) comprise a consecutive series of six to ten histidine residues. His tags are used for many recombinant proteins to facilitate purification, allowing researchers to extract a protein of interest from thousands of other proteins found in a cell or cell lysate. The small size of the 10X-His tag has a lower possibility for the tag to affect the functionality of the fusion protein. Histidine forms coordination bonds with immobilized transition metal ions, and this property can be utilized for protein purification. His-tag protein purification is by a specialized form of affinity chromatography, called immobilized metal affinity chromatography (IMAC), where proteins or peptides are separated according to their affinity for metal ions immobilized to a solid chelating resin. During this process, a small His-tag is fused to either the N or C terminus of the target protein, enabling capture by nickel or cobalt ions coordinated on a variety of resins. The small size, low cost, and ease of use have made the His-tag the most popular affinity-tag available. AAT Bioquest offers the largest collection of 6XHis, 0XHis, NTA, bis-NTA, tris-NTA and IDA reagents for His Tag detections and purifications.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of 10XHis alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 59.924 µL | 299.62 µL | 599.24 µL | 2.996 mL | 5.992 mL |
5 mM | 11.985 µL | 59.924 µL | 119.848 µL | 599.24 µL | 1.198 mL |
10 mM | 5.992 µL | 29.962 µL | 59.924 µL | 299.62 µL | 599.24 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
AMCA Alkyne | 346 | 434 | 19000 | 0.153 |
XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.11 |
ICG alkyne | 789 | 813 | 230000 | 0.076 |
Cy3B alkyne | 560 | 571 | 1200001 | 0.069 |
XFD647 Alkyne | 650 | 671 | 270000 | 0.03 |
Images
References
View all 50 references: Citation Explorer
Characterization of bifunctional alginate lyase Aly644 and antimicrobial activity of enzymatic hydrolysates.
Authors: Zhang, Ao and Cao, Zhe and Zhao, Luying and Zhang, Qian and Fu, Liping and Li, Jiang and Liu, Tao
Journal: Applied microbiology and biotechnology (2023): 6845-6857
Authors: Zhang, Ao and Cao, Zhe and Zhao, Luying and Zhang, Qian and Fu, Liping and Li, Jiang and Liu, Tao
Journal: Applied microbiology and biotechnology (2023): 6845-6857
Switch-on Fluorescence Analysis of Protease Activity with the Assistance of a Nickel Ion-Nitrilotriacetic Acid-Conjugated Magnetic Nanoparticle.
Authors: Ma, Xiaohua and Lv, Yingxin and Liu, Panpan and Hao, Yuanqiang and Xia, Ning
Journal: Molecules (Basel, Switzerland) (2023)
Authors: Ma, Xiaohua and Lv, Yingxin and Liu, Panpan and Hao, Yuanqiang and Xia, Ning
Journal: Molecules (Basel, Switzerland) (2023)
HAV-Peptides Attached to Colloidal Probes Faithfully Detect E-Cadherins Displayed on Living Cells.
Authors: Toy, Silan and Dietz, Jörn and Naumann, Peter and Trothe, Janina and Thomas, Franziska and Diederichsen, Ulf and Steinem, Claudia and Janshoff, Andreas
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202203904
Authors: Toy, Silan and Dietz, Jörn and Naumann, Peter and Trothe, Janina and Thomas, Franziska and Diederichsen, Ulf and Steinem, Claudia and Janshoff, Andreas
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202203904
Biorecognition element-free electrochemical detection of recombinant glycoproteins using metal-organic frameworks as signal tags.
Authors: Chang, Yong and Liu, Gang and Li, Shuang and Liu, Lin and Song, Qijun
Journal: Analytica chimica acta (2023): 341540
Authors: Chang, Yong and Liu, Gang and Li, Shuang and Liu, Lin and Song, Qijun
Journal: Analytica chimica acta (2023): 341540
Novel Aptamers for the Reactivation of Latent HIV.
Authors: Serumula, William and Nkambule, Bongani and Parboosing, Raveen
Journal: Current HIV research (2023)
Authors: Serumula, William and Nkambule, Bongani and Parboosing, Raveen
Journal: Current HIV research (2023)
Purification of a Recombinant Human PEX1/PEX6 AAA+ ATPase Complex from HEK293TT Cells.
Authors: Pandey, Saroj and Dodt, Gabriele
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 359-372
Authors: Pandey, Saroj and Dodt, Gabriele
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 359-372
A Simple and Versatile Strategy for Oriented Immobilization of His-Tagged Proteins on Magnetic Nanoparticles.
Authors: Castro-Hinojosa, Christian and Del Sol-Fernández, Susel and Moreno-Antolín, Eduardo and Martín-Gracia, Beatriz and Ovejero, Jesús G and de la Fuente, Jesús Martínez and Grazú, Valeria and Fratila, Raluca M and Moros, María
Journal: Bioconjugate chemistry (2023)
Authors: Castro-Hinojosa, Christian and Del Sol-Fernández, Susel and Moreno-Antolín, Eduardo and Martín-Gracia, Beatriz and Ovejero, Jesús G and de la Fuente, Jesús Martínez and Grazú, Valeria and Fratila, Raluca M and Moros, María
Journal: Bioconjugate chemistry (2023)
Efficient Purification of Polyhistidine-Tagged Recombinant Proteins Using Functionalized Corundum Particles.
Authors: Völzke, Jule L and Smatty, Sarah and Döring, Sarah and Ewald, Shireen and Oelze, Marcus and Fratzke, Franziska and Flemig, Sabine and Konthur, Zoltán and Weller, Michael G
Journal: Biotech (Basel (Switzerland)) (2023)
Authors: Völzke, Jule L and Smatty, Sarah and Döring, Sarah and Ewald, Shireen and Oelze, Marcus and Fratzke, Franziska and Flemig, Sabine and Konthur, Zoltán and Weller, Michael G
Journal: Biotech (Basel (Switzerland)) (2023)
Magnetically purification/immobilization of poly histidine-tagged proteins by PEGylated magnetic graphene oxide nanocomposites.
Authors: Khataminezhad, Ehteram Sadat and Hajihassan, Zahra and Razi Astaraei, Fatemeh
Journal: Protein expression and purification (2023): 106264
Authors: Khataminezhad, Ehteram Sadat and Hajihassan, Zahra and Razi Astaraei, Fatemeh
Journal: Protein expression and purification (2023): 106264
Poly-Histidine-Tagged Protein Purification Using Immobilized Metal Affinity Chromatography (IMAC).
Authors: Loughran, Sinéad T and Bree, Ronan T and Walls, Dermot
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 193-223
Authors: Loughran, Sinéad T and Bree, Ronan T and Walls, Dermot
Journal: Methods in molecular biology (Clifton, N.J.) (2023): 193-223