Name: ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
Brand: AAT Bioquest
SKU: 11003
Availability: InStock

ReadiUse™ TMB Substrate Solution

Optimized for ELISA Assays with HRP Conjugates

Horseradish peroxidase (HRP) and HRP conjugates facilitate the TMB oxidation in the presence of hydrogen peroxide, turning TMB into its blue oxidized product. ReadiUse™ TMB Substrate Solution is a premixed solution of TMB substrate with hydrogen peroxide. It produces a blue product upon interaction with HRP or HRP conjugates without the addition of hydrogen peroxide. The soluble blue product can be quantitated at 650 nm. Use of a stop solution enhances sensitivity 2-4 fold and the resulting yellow solution can be read at 450 nm. ReadiUse™ TMB Substrate Solution provides an convenient and ultrasensitive quantitative substrate system.

HRP dose response was measured with ReadiUse™ TMB Substrate Solution in a clear 96-well plate using a SpectraMax microplate reader (Molecular Devices).

Protocol

COA

Catalog	Size	Price	Quantity
11003	1 L	Price
11012	100 ml	Price

References

View all 42 references: Citation Explorer

Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance

Authors:

Chen X, Tian X, Su B, Huang Z, Oyama M.

Journal:

Dalton Trans (2014): 7449

NiO nanoparticles modified with 5,10,15,20-tetrakis(4-carboxyl pheyl)-porphyrin: Promising peroxidase mimetics for HO and glucose detection

Authors:

Liu Q, Yang Y, Li H, Zhu R, Shao Q, Yang S, Xu J.

Journal:

Biosens Bioelectron (2014): 147

Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates

Authors:

Aumiller WM, Jr., Davis BW, Hatzakis E, Keating CD.

Journal:

J Phys Chem B (2014): 10624

Biogenic magnetic nanoparticles from Burkholderia sp. YN01 exhibiting intrinsic peroxidase-like activity and their applications

Authors:

Pan Y, Li N, Mu J, Zhou R, Xu Y, Cui D, Wang Y, Zhao M.

Journal:

Appl Microbiol Biotechnol. (2014)

5,10,15,20-tetrakis(4-carboxyl phenyl)porphyrin-CdS nanocomposites with intrinsic peroxidase-like activity for glucose colorimetric detection

Authors:

Liu Q, Jia Q, Zhu R, Shao Q, Wang D, Cui P, Ge J.

Journal:

Mater Sci Eng C Mater Biol Appl (2014): 177

Physical properties

Molecular weight

240.34

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12171501
CAS	54827-17-7

Instrument settings

Absorbance microplate reader
Absorbance	650 nm
Recommended plate	Clear bottom

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on September 23, 2025