Name: ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*
Brand: AAT Bioquest
SKU: 11003
Availability: InStock

ReadiUse™ TMB Substrate Solution

Optimized for ELISA Assays with HRP Conjugates

ReadiUse™ TMB Substrate Solution is a colorimetric substrate solution for detecting HRP and HRP conjugates in ELISA assays.

Detection Principle: HRP-mediated oxidation of TMB produces a blue color.
Formulation: Premixed with TMB and hydrogen peroxide for direct use.
Readout: Blue product is measured at 650 nm.
Application: Optimized for ELISA assays using HRP conjugates.

HRP dose response was measured with ReadiUse™ TMB Substrate Solution in a clear 96-well plate using a SpectraMax microplate reader (Molecular Devices).

Protocol

Catalog	Size	Price	Quantity
11003	1 L	Price
11012	100 ml	Price

Overview

Horseradish peroxidase (HRP) and HRP conjugates facilitate the TMB oxidation in the presence of hydrogen peroxide, turning TMB into its blue oxidized product. ReadiUse™ TMB Substrate Solution is a premixed solution of TMB substrate with hydrogen peroxide. It produces a blue product upon interaction with HRP or HRP conjugates without the addition of hydrogen peroxide. The soluble blue product can be quantitated at 650 nm. Use of a stop solution enhances sensitivity 2-4 fold and the resulting yellow solution can be read at 450 nm. ReadiUse™ TMB Substrate Solution provides an convenient and ultrasensitive quantitative substrate system.

References

View all 42 references: Citation Explorer

Au nanoparticles on citrate-functionalized graphene nanosheets with a high peroxidase-like performance

Authors:

Chen X, Tian X, Su B, Huang Z, Oyama M.

Journal:

Dalton Trans (2014): 7449

NiO nanoparticles modified with 5,10,15,20-tetrakis(4-carboxyl pheyl)-porphyrin: Promising peroxidase mimetics for HO and glucose detection

Authors:

Liu Q, Yang Y, Li H, Zhu R, Shao Q, Yang S, Xu J.

Journal:

Biosens Bioelectron (2014): 147

Interactions of macromolecular crowding agents and cosolutes with small-molecule substrates: effect on horseradish peroxidase activity with two different substrates

Authors:

Aumiller WM, Jr., Davis BW, Hatzakis E, Keating CD.

Journal:

J Phys Chem B (2014): 10624

Biogenic magnetic nanoparticles from Burkholderia sp. YN01 exhibiting intrinsic peroxidase-like activity and their applications

Authors:

Pan Y, Li N, Mu J, Zhou R, Xu Y, Cui D, Wang Y, Zhao M.

Journal:

Appl Microbiol Biotechnol. (2014)

5,10,15,20-tetrakis(4-carboxyl phenyl)porphyrin-CdS nanocomposites with intrinsic peroxidase-like activity for glucose colorimetric detection

Authors:

Liu Q, Jia Q, Zhu R, Shao Q, Wang D, Cui P, Ge J.

Journal:

Mater Sci Eng C Mater Biol Appl (2014): 177

Physical properties

Molecular weight

240.34

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Refrigerated (2-8 °C); Minimize light exposure
CAS	54827-17-7

Instrument settings

Absorbance microplate reader
Absorbance	650 nm
Recommended plate	Clear bottom

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Page updated on October 19, 2026