Name: Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color*
Brand: AAT Bioquest
SKU: 13803
Price: 355 USD
Availability: InStock

Amplite® Colorimetric Alanine Aminotransferase Assay Kit

Blue Color

Amplite® Colorimetric Alanine Aminotransferase Assay Kit *Blue Color* is a colorimetric assay kit for quantification of alanine aminotransferase (ALT) activity in biological samples.

Detection Principle: Measures glutamate generated by ALT through an enzyme-coupled blue color reaction.
Sensitivity: Detects ALT concentrations as low as 10 mU/mL in a 100 µL reaction.
Specificity: Monitors ALT-catalyzed conversion of alanine and α-ketoglutarate to pyruvate and glutamate.
Instrument Compatibility: Read at an absorbance ratio of A570/A610 nm using a clear-bottom plate.
Applications: Suitable for ALT measurement in various biological samples.

ALT dose response was measured with Amplite Colorimetric Alanine Aminotransferase Assay Kit in a 96-well white, clear-bottom plate using a SpectraMax microplate reader (Molecular Devices).

Protocol

SDS

Catalog	Size	Price	Quantity
13803	200 Tests	Price

Overview

Alanine aminotransferase (ALT), also called serum glutamate pyruvic transaminase (GPT), is a member of transferase family. It catalyzes the reversible transfer of an alpha-amino group between alanine and glutamate, and is an important enzyme in amino acid metabolism. ALT is found mainly in liver and small amount in heart, muscle, and kidneys. In healthy subjects, serum ALT levels are low. However, when cells are damaged, such as acute and chronic hepatitis, obstructive jaundice, carcinoma of liver, myocardial infarction, ALT may leak into the blood stream and the ALT levels are significantly elevated. Therefore, determination of serum ALT level has great clinical and diagnostic significance. Amplite® Colorimetric Alanine Aminotransferase Assay Kit provides a quick and sensitive method for the measurement of ALT in various biological samples. ALT catalyzes the reaction of alanine and α-ketoglutarate to pyruvate and glutamate. The product glutamate is measured by the generation of a blue color product through an enzyme coupled reaction cycle. The signal can be read by an absorbance microplate reader at an absorbance ratio of A570 nm to A610 nm. With the Amplite® Colorimetric Alanine Aminotransferase Assay Kit as little as 10 mU/mL ALT was detected in a 100 µL reaction volume. The assay is robust, and can be readily adapted for a wide variety of applications.

Citations

View all 9 citations: Citation Explorer

Decreased autophagosome biogenesis, reduced NRF2, and enhanced ferroptotic cell death are underlying molecular mechanisms of non-alcoholic fatty liver disease

Authors:

Liu, Pengfei and Anandhan, Annadurai and Chen, Jingjing and Shakya, Aryatara and Dodson, Matthew and Ooi, Aikseng and Chapman, Eli and White, Eileen and Garcia, Joe GN and Zhang, Donna D

Journal:

Redox Biology (2022): 102570

Reparative Efficacy of Liposome-Encapsulated Oleanolic Acid against Liver Inflammation Induced by Fine Ambient Particulate Matter and Alcohol in Mice

Authors:

Wei, Ching-Ting and Wang, Yu-Wen and Wu, Yu-Chiuan and Lin, Li-Wei and Chen, Chia-Chi and Chen, Chun-Yin and Kuo, Shyh-Ming

Journal:

Pharmaceutics (2022): 1108

LIPOSOMAL TECHNOLOGIES TO IMPROVE GENE DELIVERY

Authors:

Nardo Padron, David

Pharmacologic IRE1/XBP1s activation promotes systemic adaptive remodeling in obesity

Authors:

Madhavan, Aparajita and Kok, Bernard P and Rius, Bibiana and Grandjean, Julia and Alabi, Adekunle and Albert, Verena and Sukiasyan, Ara and Powers, Evan T and Galmozzi, Andrea and Saez, Enrique and others,

Journal:

Nature communications (2022): 1--9

Reparative and toxicity-reducing effects of liposome-encapsulated saikosaponin in mice with liver fibrosis

Authors:

Shiu, Li-Yen and Huang, Han Hsiang and Chen, Chun Yin and Cheng, Hsia-Ying and Chen, Chih I and Kuo, Shyh Ming

Journal:

Bioscience Reports (2020)

References

View all 226 references: Citation Explorer

Pegylated interferon alpha-2b plus ribavirin for Japanese chronic hepatitis C patients with normal alanine aminotransferase

Authors:

Kainuma M, Furusyo N, Azuma K, Kajiwara E, Takahashi K, Nomura H, Tanabe Y, Satoh T, Maruyama T, Nakamuta M, Kotoh K, Shimoda S, Hayashi J.

Journal:

Hepatol Res (2012): 33

Coexistence of non-alcoholic fatty liver disease with elevated alanine aminotransferase is associated with insulin resistance in young Han males

Authors:

Wang R, Lu Q, Feng J, Yin F, Qin C, Liu B, Liu Y, Liu X.

Journal:

Endocrine (2012): 70

Levels of Alanine Aminotransferase Confound Use of Transient Elastography to Diagnose Fibrosis in Patients with Chronic HCV Infection

Authors:

Tapper EB, Cohen EB, Patel K, Bacon B, Gordon S, Lawitz E, Nelson D, Nasser IA, Challies T, Afdhal N.

Journal:

Clin Gastroenterol Hepatol. (2012)

Association of serum alanine aminotransferase and gamma-glutamyltransferase levels within the reference range with metabolic syndrome and nonalcoholic fatty liver disease

Authors:

Oh HJ, Kim TH, Sohn YW, Kim YS, Oh YR, Cho EY, Shim SY, Shin SR, Han AL, Yoon SJ, Kim HC.

Journal:

Korean J Hepatol (2011): 27

Updated thresholds for alanine aminotransferase do not exclude significant histological disease in chronic hepatitis C

Authors:

Sanai FM, Helmy A, Dale C, Al-Ashgar H, Abdo AA, Katada K, AlMana H, Saadeh M, Al-Hussaini H, AlQuaiz M, Hashem A, AlSwat K, Bzeizi KI, Marotta PJ.

Journal:

Liver Int (2011): 1039

Usage and storage

Intended use

Research Use Only (RUO)

Instrument settings

Absorbance microplate reader
Absorbance	570/610 nm
Recommended plate	Clear bottom

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Fax
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Page updated on October 18, 2026

Component A: ALT Enzyme Mix	1 bottle (lyophilized powder)
Component B: ALT Assay Buffer	bottle (10 mL)
Component C: NAD	1 vial
Component D: ALT Positive Control	1 vial (10 U)