3'-DABCYL CPG *1000 Å*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | N/A |
| Solvent | MeCN |
Spectral properties
| Absorbance (nm) | 454 |
| Correction Factor (280 nm) | 0.516 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
| Overview |  SDSProtocol |
See also: Custom Oligonucleotide Labeling, Digital PCR, Polymerase Chain Reaction (PCR), Real-Time PCR (qPCR), Reverse Transcription PCR (RT-PCR), PCR Detection of Viral DNA/RNA
Molecular weight N/A | Absorbance (nm) 454 | Correction Factor (280 nm) 0.516 |
DABCYL is one of the most commonly used dark quenchers for labeling oligos and peptides. The resulted conjugates are widely used as diagnostic probes such as Molecular Beacons and protease substrates. Because of its relative stability, DABCYL can be easily introduced at many positions within a Molecular Beacon during synthesis: at the 5'-end using 5'- DABCYL-phosphoramidite, at the 3'-end using 3'- DABCYL CPGs. The resulted oligo can be deprotected in the usual way. AAT Bioquest offers the most complete set of DABCYL derivatives for preparing FRET peptides and oligos.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 454 |
| Correction Factor (280 nm) | 0.516 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 3'-Fluorescein CPG *1000 Å* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Images
Figure 1. Chemical structure for 3'-DABCYL CPG *1000 Å*
Citations
View all 1 citations: Citation Explorer
Protein N-terminal processing: substrate specificity of Escherichia coli and human methionine aminopeptidases
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599
Authors: Xiao, Qing and Zhang, Feiran and Nacev, Benjamin A and Liu, Jun O and Pei, Dehua
Journal: Biochemistry (2010): 5588--5599
References
View all 34 references: Citation Explorer
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Journal: Protein Expr Purif (2006): 88
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Journal: Anal Biochem (2006): 110
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Journal: Curr Top Med Chem (2006): 361
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Journal: Acta Biochim Pol (2004): 81
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