California Red™ SE

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	814.92
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.194
Extinction coefficient (cm ^-1 M ^-1)	100000¹
Excitation (nm)	592
Emission (nm)	609

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

814.92

Correction Factor (260 nm)

0.24

Correction Factor (280 nm)

0.194

Extinction coefficient (cm ^-1 M ^-1)

100000¹

Excitation (nm)

592

Emission (nm)

609

Although Texas Red® is the most popular labeling reagent of sulfonyl chloride, it is quite unstable in water. Even in anhydrous DMF, Texas Red® tends to give a very complicated reaction mixture. It reacts with thiols, alcohols, phenols, aliphatic amines and aromatic amines indiscriminatingly. California Red™ is a succinimidyl ester and has the spectral properties identical to those of Texas Red®. California Red™ is a superior replacement for Texas Red®. California Red™ only reacts with aliphatic amines such as amino acids, peptides and proteins to give bright red fluorescent conjugates that are extremely stable. Compared to Texas Red, California Red has much higher labeling efficiency, and more importantly the resulted conjugates are much easier to be purified due to its much cleaner reactions. We strongly recommend that you consider using California Red™ to replace Texas Red® for labeling peptides and oligonucleotides.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of California Red™ SE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	122.711 µL	613.557 µL	1.227 mL	6.136 mL	12.271 mL
5 mM	24.542 µL	122.711 µL	245.423 µL	1.227 mL	2.454 mL
10 mM	12.271 µL	61.356 µL	122.711 µL	613.557 µL	1.227 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Correction Factor (260 nm)	0.24
Correction Factor (280 nm)	0.194
Extinction coefficient (cm ^-1 M ^-1)	100000¹
Excitation (nm)	592
Emission (nm)	609

Product Family

Name	Excitation (nm)	Emission (nm)	Correction Factor (260 nm)	Correction Factor (280 nm)
Sunnyvale Red™ SE Superior 6-ROX Replacement	591	624	-	-
LRB Red™ SE	558	575	0.21	0.171

Images

Figure 1. Conjugation and spectral comparison of California Red™ and Texas Red under the same conditions.

References

View all 63 references: Citation Explorer

Fluorescence properties of fluorescein, tetramethylrhodamine and Texas Red linked to a DNA aptamer
Authors: Unruh JR, Gokulrangan G, Wilson GS, Johnson CK.
Journal: Photochem Photobiol (2005): 682

A comparative study of the potential of solid triglyceride nanostructures coated with chitosan or poly(ethylene glycol) as carriers for oral calcitonin delivery
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Journal: Eur J Pharm Sci (2005): 133

Bead-based cellular analysis, sorting and multiplexing
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Journal: Chembiochem (2005): 1341

Transepithelial transport of fluorescent p-glycoprotein and MRP2 substrates by insect Malpighian tubules: confocal microscopic analysis of secreted fluid droplets
Authors: Leader JP, O'Donnell MJ.
Journal: J Exp Biol (2005): 4363

Purification and fluorescent labeling of the human serotonin transporter
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Journal: Biochemistry (2005): 3494

Budding dynamics of multicomponent tubular vesicles
Authors: Li L, Liang X, Lin M, Qiu F, Yang Y.
Journal: J Am Chem Soc (2005): 17996

Emission characteristics of fluorescent labels with respect to temperature changes and subsequent effects on DNA microchip studies
Authors: Liu WT, Wu JH, Li ES, Selamat ES.
Journal: Appl Environ Microbiol (2005): 6453

Orientational dynamics and dye-DNA interactions in a dye-labeled DNA aptamer
Authors: Unruh JR, Gokulrangan G, Lushington GH, Johnson CK, Wilson GS.
Journal: Biophys J (2005): 3455

Measurement of normal and anomalous diffusion of dyes within protein structures fabricated via multiphoton excited cross-linking
Authors: Basu S, Wolgemuth CW, Campagnola PJ.
Journal: Biomacromolecules (2004): 2347

Suppression of protein kinase Calpha triggers apoptosis through down-regulation of Bcl-xL in a rat hepatic epithelial cell line
Authors: Hsieh YC, Jao HC, Yang RC, Hsu HK, Hsu C.
Journal: Shock (2003): 582